Methods are provided for refining natural oil feedstocks. The methods comprise reacting the feedstock in the presence of a metathesis catalyst under conditions sufficient to form a metathesized product comprising olefins and esters. In certain embodiments, the methods further comprise separating the olefins from the esters in the metathesized product. In certain embodiments, the methods further comprise hydrogenating the olefins under conditions sufficient to form a fuel composition. In certain embodiments, the methods further comprise transesterifying the esters in the presence of an alcohol to form a transesterified product.

The present invention relates to isolated polypeptides having phospholipase C activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Provided are methods and systems for recovering protein product powder, purified water and omega-3 oil from an animal tissue. The methods and systems use high throughput extraction filtration separation systems. Animal tissue, for example fish, and organic solvent are directly or indirectly transferred into one of the optional extraction or filtration systems. The extraction-filtration systems provide a high degree of filtration performance and product washing efficiency. Each system ultimately yields a product wet cake that includes the protein product. The protein product wet cake is then further dried in a drying unit to yield the final protein powder product. In each system, the process filtrates undergo further processing by filtration and distillation to recover the organic solvent and separate out the omega-3 fish oil. The recovered organic solvent can be recycled back into the process. Solid protein product powder is thus recovered, along with omega-3 oil, purified water and recovered solvent.

The present invention relates to a polyunsaturated fatty acid composition comprising Omega-3 fatty acids, 17-HDHA and 18-HEPE. The composition can furthermore comprise DPA and/or an acceptable carrier and can be present in a capsule or other suitable dosage unit. The invention also relates to the process of obtaining the composition and methods for using same.

Provided is a method for producing a concentrated oil-based polyphenol composition for human consumption. The unique combination of polyphenols in a biologic oil-based formulation may be administered for optimal effects on human platelets, lipids, and inflammatory markers. These physiologic changes have beneficial effects for human health and longevity.

A system for further filtering a liquid extract material after an initial extraction process. In an embodiment, the system includes a semi-rigid filter cup that is substantially cylindrical, the filter cup having a top and a bottom, wherein the top is oriented to receive filter media prior to a filtration process, and material to be filtered during the filtration process. The filter cup includes an integrated deformable flange at the top of the filter cup, an integrated top o-ring that is integrated into the top of the integrated deformable flange and an integrated bottom o-ring that is integrated into the bottom of the integrated deformable flange.

A novel method is developed to remove wax and terpene from the wild hemp plants to produce enriched cannabidiol (CBD) crude oil with high quality and further purify the crude oil into crystal/isolate with 99.0% content of CBD. The method utilizes titanium dioxide (TiO.sub.2) photocatalyst for redox reaction under the irradiation of UV (ultraviolet) light in a solvent extraction process of CBD, which significantly removes accompanying wax and terpene. With the beneficial photocatalyst property, the TiO.sub.2 decomposes the wax and terpene in the wild hemp crude extract through appropriate UV radiation to induce catalytic reaction at specific PH (potential hydrogen) levels. Through optimizing the photocatalyst dewaxing using TiO.sub.2, the method achieves the best dewaxing effect and maximum terpene reduction. Thus, the method offers a low-cost, reusable, and biologically friendly process of removing wax and terpene to maximize the efficiency and effectiveness of the separation processes in the CBD production.

A process for production of biofuels from algae can include cultivating an oil-producing algae by promoting sequential photoautotrophic and heterotrophic growth. The method can further include producing oil by heterotrophic growth of algae wherein the heterotrophic algae growth is achieved by introducing a sugar feed to the oil-producing algae. An algal oil can be extracted from the oil-producing algae, and can be converted to form biodiesel.

In an aspect, a method is disclosed that includes contacting a composition with an aqueous solution to yield a mixture, where the composition includes one or more of animal fats, animal oils, plant fats, plant oils, vegetable fats, vegetable oils, greases, and used cooking oil, about 5 wt. % or more of free fatty acids, about 10 wppm or more of total metals, about 8 wppm or more phosphorus, about 20 wppm or more of nitrogen, and the aqueous solution includes ((NH.sub.4).sub.2H.sub.2EDTA, (NH.sub.4).sub.4EDTA, a monoammonium salt of diethylenetriaminepentaacetic acid, a diammonium salt of diethylenetriaminepentaacetic acid, a triammonium salt of diethylenetriaminepentaacetic acid, a tetraammonium salt of diethylenetriaminepentaacetic acid, (NH.sub.4).sub.5DTPA, a combination of citric acid and Na.sub.4EDTA, a combination of citric acid and Na.sub.2H.sub.2EDTA, a combination of citric acid and a monosodium salt of diethylenetriaminepentaacetic acid, a combination of citric acid and a disodium salt of diethylenetriaminepentaacetic acid, a combination of citric acid and a trisodium salt of diethylenetriaminepentaacetic acid, a combination of citric acid and a tetrasodium salt of diethylenetriaminepentaacetic acid, a combination of citric acid and Na.sub.5DTPA, or a combination of any two or more thereof, where the method further includes centrifuging the mixture to yield a first treated composition, wherein the first treated composition has less total metals and less phosphorus than the composition.

The present invention relates generally to corn dry-milling, and more specifically, to a method and system for removing insoluble solids mid-evaporation in a corn (or similar carbohydrate-containing grain) dry milling process for making alcohol, such as ethanol, and/or other biofuels/biochemicals. In one example, the method for removing residual insoluble solids in a grain dry milling process includes separating a whole stillage byproduct into an insoluble solids portion and a solubles portion, which includes residual insoluble solids. Then, the solubles portion is subjected to a first evaporation, via one or more evaporators, to remove liquid from the solubles portion to define a concentrated solubles portion. After the first evaporation, the residual insoluble solids are separated from the concentrated solubles portion. And thereafter, the concentrated solubles portion is subjected to a second evaporation, via one or more evaporators, to remove additional liquid from the concentrated solubles portion.