The present invention provides a method of efficiently separating solid fat from polyunsaturated fatty acid grease. The method comprises adding a certain amount of inert material to the polyunsaturated fatty acid grease, and cooling crystallization, and then filtering through a filter to obtain clarified polyunsaturated fatty acid grease. Afterwards introducing hot gas through the filter to make solid fat adsorbed on a filter cake in the filter dissolved and then recycling it through a filter plate. Inert solid substances can be applied next time. The method of the present invention can be efficiently separated saturated and low-saturated fatty acids from polyunsaturated fatty acid grease. In the process, the winterization crystallization time is short, the crystallization is complete, the crystal separation is easy. It will not cause some solid fat melted and thus re-enter the filtrate due to long separation and blowing dry in conventional process. The yield of final product is high up to 89.8%-97.8%. After winterization, a lipid clarity of the polyunsaturated fatty acid grease is good, and no crystal precipitation would occur at 0° C. for 5 hrs.

The present invention relates to a process for preparing a deodorized oil with in-situ prepared mono-acylglycerides and the oil obtained as such. It relates to a process for preparing a deodorized oil containing in-situ prepared mono-acylglycerides by interesterifying in presence of an enzyme a glyceride containing fraction wherein the ratio of free and esterified hydroxyl groups (OH) to free and esterified fatty acids (FA) is greater than 1.07 and obtaining an oily composition containing less than 1% w/w, preferably less than 0.5% free fatty acids. Furthermore it relates to a deodorized oil containing 30 to 70% triglycerides, 20 to 50% di-acyl glycerides, 1.5 to 25% mono-acyl glycerides.

The present invention relates to a process for preparing a deodorized oil with in-situ prepared mono-acylglycerides and the oil obtained as such. It relates to a process for preparing a deodorized oil containing in-situ prepared mono-acylglycerides by interesterifying in presence of an enzyme a glyceride containing fraction wherein the ratio of free and esterified hydroxyl groups (OH) to free and esterified fatty acids (FA) is greater than 1.07 and obtaining an oily composition containing less than 1% w/w, preferably less than 0.5% free fatty acids. Furthermore it relates to a deodorized oil containing 30 to 70% triglycerides, 20 to 50% di-acyl glycerides, 1.5 to 25% mono-acyl glycerides.

The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The disclosure discloses an enzymatic method for preparation of lecithin polyunsaturated fatty acids (PUFAs), and belongs to the technical field of separation and application of enzyme. A heat treatment procedure is added after a reaction substrate is in contact with an enzyme to adjust the ratio of sn-1 lysophospholipid PUFAs to sn-2 lysophospholipid PUFAs in a reaction product and to promote the production of sn-2 lysophospholipid PUFAs, thereby promoting the production of lecithin PUFAs, which greatly increases the production efficiency of lecithin PUFAs and the lecithin PUFA content in the product. With simple operations and high reaction rate, the method can significantly increase the content of lecithin PUFAs in the product, can effectively avoid the oxidation of PUFA, and has high economic benefits and promising industrial application prospects.

[Problem] To provide: a method for suppressing the coloration of oils and fats during frying; and a coloring inhibitor. [Solution] A method for suppressing the coloration of an oil/fat composition for frying, the method comprising a step for adding a prepared oil to an edible oil or fat, wherein the prepared oil is obtained through (1) a degumming step, (2) a neutralization step which may or may not be performed, (3) a bleaching step which may or may not be performed, and (4) a deodorizing step, in this order, in the process of refining a crude oil obtained from an oil feedstock, and the absorbance difference determined by subtracting the absorbance at a wavelength of 750 nm from the absorbance at a wavelength of 660 nm of the prepared oil obtained from step (3) is at least 0.030 when isooctane is used as the control. A coloring inhibitor characterized by containing said prepared oil.

A method to purify black liquor soap (BLS) from sulfur compounds is disclosed. In the BLS desulfurization method, a BLS composition is heated in an inert atmosphere and sulfur is removed via aqueous extractions with inorganic base in the absence of other chemicals such as brine, sulfate salts and carbonate salts. The purified BLS composition exhibits favorable properties, including reduced sulfur content, lower color, and improved odor characteristics. The resulting tall oil compositions, depitched tall oil compositions, tall oil distillation fractions, and products derived therefrom also show reduced sulfur content, lower color, and improved odor characteristics.

The present technology provides a method that includes contacting a composition with a caustic solution to produce a caustic-treated composition; combining the caustic-treated composition with silica particles to produce a slurry; and removing the silica particles from the slurry to produce a treated composition; wherein the composition includes one or more of animal fats, animal oils, plant fats, plant oils, vegetable fats, vegetable oils, greases, and used cooking oil and the composition includes: at least about 10 wppm of total metals, at least about 8 wppm of phosphorus, at least about 10 wppm of chlorine, at least about 10 wppm of sulfur, at least about 20 wppm of nitrogen, at least about 5 wt. % of free fatty acids; and has an acid number from about 10 mg KOH/g to about 150 mg KOH/g, and the silica particles has a particle size from about 10 microns to about 50 microns, a BET surface area from about 200 m.sup.2/g to about 1000 m.sup.2/g.

The disclosure relates to a Cannabis seed processing method and system comprising ultrasonic treatment of Cannabis seeds coupled to a membrane extraction system for efficient recovery of hemp seed oil.

A method for decolorization and deodorization of egg yolk oil comprises at least: adding a first ether solution to a crude egg yolk oil, and performing a first stirring process, to produce a first egg yolk oil mixture; adding activated carbon to the first egg yolk oil mixture, performing a second stirring process, and removing the activated carbon, to produce a second egg yolk oil mixture; adding a second ether solution and distilled water to the second egg yolk oil mixture, and performing a third stirring process, to produce a third egg yolk oil mixture; and removing distilled water, part of the first ether solution, and part of the second ether solution from the third egg yolk oil mixture by a distillation process, and removing the remaining first ether solution and the remaining second ether solution by a vacuum process to produce a decolorized and deodorized egg yolk oil.