A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA, a microbial cell containing a PUFA, and use of the microbial cell are provided. A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA including culture of a microorganism capable of producing arachidonic acid (ARA) and/or dihomo-gamma-linolenic acid (DGLA) is provided, the method including at least one of the following steps: (a) adding an organic acid in an amount of 0.01 to 5 w/v % to a culture medium after the beginning of main culture; (b) increasing the pH of the culture medium to a range effective for culture after the beginning of the main culture; and (c) adding a metal sulfate in an amount of 0.01 to 0.5 w/w % to the main culture medium.

The present invention relates to processes of recovering oil after liquefaction and/or from thin stillage and/or syrup/evaporated centrate from a fermentation product production process by adding a thermostable protease to the whole stillage, thin stillage and/or syrup.

A method for extracting naturally occurring oil bodies (oleosomes) from a material containing naturally occurring oil bodies (oleosomes) includes the steps of dispersing the material containing oleosomes in an aqueous composition to thereby obtain an aqueous dispersion of material containing oleosomes, extracting oleosomes from the aqueous dispersion of material containing oleosomes to thereby obtain a crude oleosome extract and isolating crude oleosomes from the extract by drying the crude oleosome extract. The dried oleosome powder may be used with a personal care product, a pharmaceutical product or a food product.

The invention relates to a process for enzymatic degumming of unrefined triglyceride oil, said process comprising the following successive steps: (a) providing an unrefined triglyceride oil having a phosphorus content of at least 100 mg per kg of unrefined triglyceride oil; (b) combining the unrefined triglyceride oil with water, an acid and a phospholipase to produce an oil-in-water emulsion having a pH in the range of 2.5 to 4.5; said phospholipase being selected phospholipase A1, phospholipase A2 and combinations thereof; (c) keeping the emulsion at a temperature of 20-90° C. for at least 10 minutes; (d) introducing a base into the emulsion; and (e) separating degummed triglyceride oil from the emulsion. This enzymatic degumming process is extremely effective in removing phospholipids, including non-hydratable phospholipids (NHP), from unrefined vegetable oils and produces degummed vegetable oil in high yield.

The present invention relates to a method for manufacturing cooking oil, and cooking oil and food manufactured using the same, wherein the manufacturing method includes a) roasting a vegetable raw material by far infrared rays, b) expressing the roasted vegetable raw material to obtain an oil or fat composition containing a first active ingredient, c) obtaining an extract containing a second active ingredient from a residue of the vegetable raw material remaining after the oil or fat composition is expressed, and d) mixing the oil or fat composition with the extract.

The present invention relates to processes for producing industrial products such as hydrocarbon products from non-polar lipids in a vegetative plant part. Preferred industrial products include alkyl esters which may be blended with petroleum based fuels.

PROBLEM To provide a composition comprising highly enriched PUFA or its alkyl esters while containing fatty acid esters of 3-MCPD at adequately low concentrations and to provide an efficient method for producing the composition.

MEANS FOR SOLVING A composition that contains fatty acids or fatty acid alkyl esters as its major component, the composition containing highly unsaturated fatty acid or alkyl ester thereof, wherein the proportion of the highly unsaturated fatty acid in the constituent fatty acids of the composition is 50 area % or more and wherein the concentration of 3-MCPD as found upon analyzing the composition by American Oil Chemists' Society official method Cd 29b-13 assay A is less than 1.80 ppm.

The current invention relates to a process for in-creasing the yield of an isolated oleosome composition and the process is comprising blending of a processed fiber fraction of an oleosome source with at least one other fraction of an oleosome source. The invention relates to an isolated oleosome composition characterized in that it has a fiber content of from 0.5 to 15%, a particle size of the solids from 2 to 200 micron, and an oil content of 20 to 95% on total dry matter of which at least 60% is present as oleosomes. The invention relates to food and feed products, pharmaceutical products, personal care products, nutritional compositions and industrial products comprising the isolated oleosomes composition. The invention relates to the use of a fiber fraction of an oleosome source to increase the yield of the isolated oleosome composition.

The present invention addresses the problem of providing an oil/fat in which the glycidol content and glycidol fatty acid ester content are reduced. A method for manufacturing an edible fat/oil, wherein a step for decolorizing and deodorizing an oil/fat that contains a palm-based oil/fat, in which physically refined palm oil (RBDPO) is used as a raw material, includes a step for performing an acid-induced contacting process during a decolorization work step.

Embodiments described herein provide a method, comprising routing a bio-oil to a mixing device; routing a wash material to the mixing device; using the mixing device to form a mixture from the bio-oil and the wash material; routing the mixture to an electrostatic separator; and applying an electric field to the mixture, in the electrostatic separator, to separate the wash material from the bio-oil.