The present invention provides a method for vegetable oil deacidification by enzymatic amidation, which relates to the field of oil refining technology. The present invention is carried out through mixing high acid value vegetable oil with ethanolamine at a certain molar ratio in a solvent or solvent-free system, adding with a certain amount of lipase, and reacting at certain temperature for a period of time. The monoethanolamine has been used as an acyl donor for the first time to react with free fatty acid, which avoids the increasing amount of by-products and great loss of neutral oil in reaction that involved with triglycerides. The method of the present invention has the advantages of high selectivity, high catalytic efficiency, and environment friendly in the reaction. From enzymes recycling, it greatly reduces costs, which shows tremendous potential in the application.

The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.

Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition, treating the lysed cell composition to form an oil-containing emulsion and then recovering the oil from the oil-containing emulsion. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

A method for treating edible cooking oil which significantly expands the life of the edible cooking oil used to deep fry, wherein a rested combination of diatomaceous earth and anhydrous magnesium silicate are used as a frying and filtering aid.

A method for purifying low-quality glyceridic material usually unfit for use as feed or food includes a thermal treatment of the low-quality glyceridic material and subsequent chemical treatments. Alkali containing compounds, such as soaps, and glycerol, can be added to the low-quality glyceridic prior to its thermal treatment.

The present invention relates to a process for treating an oil comprising a chlorophyll substrate, the process comprising contacting the oil with a polypeptide having decolorase activity or a composition comprising the polypeptide, wherein the polypeptide is selected from the group consisting of: a. a polypeptide which has at least 80% identity to amino acids 1 to 318 of SEQ ID NO: 1; and, b. a polypeptide encoded by a nucleic acid sequence that has at least 80% identity to the nucleic acid sequence of SEQ ID NO: 2.

Disclosed is a VE Enrichment System, comprising a falling film heat exchanger, a deodorization tower (deodorizer), a deodorizer transfer pump, a post-stripping deodorization tower (post-stripper), a high pressure steam heater and fatty acid collection unit. The falling film heat exchanger comprises of 2 shell heat exchange section of which the bottom section is connected to the deodorizer and the upper section is connected to the high pressure steam heater which is then connected to the post stripper; the post stripper bottom outlet is connected back to the falling film heat exchanger at it tube side. The post stripper top outlet (vapour outlet) is connected to the fatty acid collection unit. The upper shell section of the falling film heat exchanger and the high pressure steam heater forms a loop with the post stripper, while another loop is formed for the bottom of shell section of the falling film heat exchanger and the deodorizer and its transfer pump. Depending on operation needs, a vacuum ejector can be added. This VE enrichment system is used to produce more fatty acid distillate with higher VE content during the deodorization process of vegetable oil.

Provided herein are systems and methods for the facile extraction and purification of oils from plant material, including cannabis and hemp. The systems and methods herein are versatile, and may utilize a wide range of solvents to extract oils from a variety of plant-based material. Further, the provided systems and methods are closed loop, reducing the loss solvent and decreasing the risk of safety concerns such as human exposure to solvent chemicals or explosion of volatiles. In some embodiments, the systems and methods remove impurities from the extracted oils, for example waxes or other precipitates, and provide a higher purity and higher quality extract.

The present disclosure provides compositions and methods for enzymatic oil degumming.

Membrane-based method of washing and deacidification of oils, wherein a stream of oil is conveyed from an oil reservoir along one side of porous hydrophobic membrane, and washing aqueous solution is conveyed along another side of this membrane. The membranes form hollow fibers, and their total surface area and porosity are large enough for efficient removal of fatty acids, water, ions and hydrophilic organic impurities from oil. Membrane pore size is small enough, so that hydrodynamic mixing of oil and aqueous solution does not take place. Additional stabilization of oil/water meniscus in the pores is achieved by transmembrane pressure difference.