The present invention relates to the use of at least one glycosidase of the class EC 3.2.1.21, EC 3.2.1.4, EC 3.2.1.7, and/or EC 3.2.1.80 for hydrolyzing at least one glycosiding bond in at least one substrate selected from the group of acylated steryl glycosides of the general formula (I): R.sup.1COO[C.sub.mH.sub.nO.sub.x].sub.yOR.sup.2 wherein R.sub.1 is a saturated or non-saturated C.sub.1-C.sub.30 alkyl, R.sub.2 is a sterol moiety, m=5-7, n=9-14 and x=5-7, y=1-5. The present invention relates further to a method of for hydrolysing steryl gylcosides using said enzymes in biofuel, oil and/or fat.

The invention is methodology of producing a variety of cannabis derivatives such as THC, CBD, Delta-11, and budder. The technique involves the steps of: grinding a whole cannabis plant, including the root ball thereof, freezing the ground plant, soaking the frozen ground plant in a selected solvent, and, removing the solvent, thereby producing desired derivative of cannabis. Solvents include coconut oil, olive oil, vegetable oils, avocado oil, and seed oils.

A process for production and extraction of dihydrolipoic acid (DHLA) relates to the biotransformation of R-lipoic acid to dihydrolipoic acid. The process ferments the lipoic acid with bacteria selected from the genus of any of the following bacteria: Lactobacillus, Enterococcus, Pediococcus, or Bacillus. More particularly the present invention relates to a method for the extraction of DHLA utilizing a non-traditional solvent system, namely a food oil. Generally the DHLA that is produced in the present method is isolated from cells which have been inactivated or killed.

The present invention relates generally to the field of recombinant fatty acid synthesis, particularly in transgenic plants. The application describes genes involved in fatty acid synthesis and provides methods and vectors for the manipulation of fatty acid composition of plant oils. In particular, the invention provides constructs for achieving the integration of multiple heterologous genes involved in fatty acid synthesis into the plant genome, such that the resulting plants produce altered levels of polyunsaturated fatty acids. Also described are methods for enhancing the expression of fatty acid biosynthesis enzymes by co-expressing a silencing suppressor within the plant storage organ.

A process and apparatus for physically refining a vegetable oil comprising a step of deodourising a crude vegetable oil by steam refining under refining conditions effective to produce a refined vegetable oil having a trans fat content of less than 0.99 wt %. The apparatus may comprise a plurality of volatilisation stages for removal of free fatty acids from a vegetable oil.

The present invention relates to a process for preparing purified vegetable liquid oil, and the process is comprising contacting a vegetable liquid oil, which has not been subjected to a deodorization step, with an adsorbent comprising alumina oxide and wherein the adsorbent has a content of alumina oxide of not more than 9.5% and the obtained deodorized vegetable liquid oils.

A method for producing renewable diesel includes introducing a primary feedstock comprising biologically-derived triglycerides with catalyst poisons into a first reaction chamber and hydrolyzing the primary feedstock within the first reaction and liquid-liquid extraction chamber for at least an hour such that the reacted triglycerides are separated into an aqueous solution comprising glycerol and catalyst poisons, and an intermediate feedstock comprising free fatty acids and catalyst poisons. The method also includes distilling the intermediate feedstock to separate the intermediate feedstock into a purified intermediate stream and a lower volume bottom stream containing unreacted triglyceride, diglyceride, monoglyceride, FFA and catalyst poisons. The method also includes combining the purified intermediate feedstock with a hydrogen stream and converting, in a second reaction chamber comprising a metallic catalyst bed, the purified intermediate feedstock into a product comprising long-chain alkanes. The method also includes hydrotreating the purified intermediate feedstock into a renewable diesel product.

A method for pretreating and purifying crude bio-feedstocks intended for processes of transformation into biofuels is described, the method being characterized in that said the crude bio-feedstock is subjected, in the form of an aqueous emulsion to a heat treatment by passing it through a coil visbreaker furnace at a temperature greater than 100 C. and a pressure greater than 3 barg, possibly in an inert atmosphere, for a pre-established contact/residence time, the effluent exiting the reactor being subsequently subjected to at least a non-absorbent physical separation to separate the bio-feedstock from metal contaminants and phosphorus.

The present disclosure relates methods and systems for refining grain oil compositions using water, and related compositions produced therefrom. The present disclosure also relates to methods of using grain oil derived from a fermentation product in an anti-foam composition and/or feedstock to make diesel.

The present disclosure relates methods and systems for refining grain oil compositions using water, and related compositions produced therefrom. The present disclosure also relates to methods of using grain oil derived from a fermentation product in an anti-foam composition and/or feedstock to make diesel.