The invention as disclosed herein provides a method for purifying a non-antibody protein from solution, comprising a chromatography step wherein the solution is passed over an affinity construct containing an affinity ligand-coupled solid support, wherein the affinity construct is associated with a bioprocess unit operation, and isolating the non-antibody protein from solution.

Provided is a method for recovering linalool from hops at a high rate in the process of extraction with supercritical carbon dioxide. Also provided is a hop extract containing linalool in a high proportion.

In a method for obtaining a hop extract using supercritical carbon dioxide, the pressure of carbon dioxide in an extraction tank is adjusted to be in the range of 8 to 10 MPa, and the temperature of a separation tank is adjusted to not higher than 30 C. According to this method, an extract having a high ratio of the amount of linalool to that of -acids can be obtained. The recovery rate of linalool from hops is not lower than 33 mass %. The extract can be used as an additive for production of foods and beverages.

Provided is a method for recovering linalool from hops at a high rate in the process of extraction with supercritical carbon dioxide. Also provided is a hop extract containing linalool in a high proportion.

In a method for obtaining a hop extract using supercritical carbon dioxide, the pressure of carbon dioxide in an extraction tank is adjusted to be in the range of 8 to 10 MPa, and the temperature of a separation tank is adjusted to not higher than 30 C. According to this method, an extract having a high ratio of the amount of linalool to that of -acids can be obtained. The recovery rate of linalool from hops is not lower than 33 mass %. The extract can be used as an additive for production of foods and beverages.

A method for producing a beer or a beer-like beverage with a pure and mild hop aroma having a slight raw hop odor, which includes maintaining a hop-mixed liquid in a tank, which liquid contains a hop and a solvent, at a temperature less than the boiling point of the solvent, wherein at least a part of the hop-mixed liquid is withdrawn from the tank, and the liquid is supplied to an empty portion of the tank, again, to circulate the hop-mixed liquid through the tank. Also disclosed is an apparatus for producing the beer or beer-like beverage.

A method for extracting resins and oils from a plant includes: 1) tumbling, inside a container at a temperature of at most 50 degrees Fahrenheit with a tumbler, plant fibers having one or more portions that contain resins and/or oils; 2) colliding the tumbler with the plant fibers to release the one or more portions that contain resins and/or oils from the remainder of the plant fibers; and 3) segregating the one or more portions that contain resins and/or oils from the remainder of the plant fibers.

Disclosed herein is a method for reducing menopausal symptoms. The method may comprise preparing a topical formulation of hops extract, and administering the topical formulation on to a part of skin. Moreover, preparing the topical formulation of hops extract may include preparing the hops extract, encapsulating the hops extract in cyclodextrin to form hops nanoparticles, and dispersing the hops nanoparticles with a concentration between 5 and 15 percent by weight of the formulation in an ointment-based excipient to form the topical formulation of hops extract.

Disclosed herein is a method for reducing menopausal symptoms. The method may comprise preparing a topical formulation of hops extract, and administering the topical formulation on to a part of skin. Moreover, preparing the topical formulation of hops extract may include preparing the hops extract, encapsulating the hops extract in cyclodextrin to form hops nanoparticles, and dispersing the hops nanoparticles with a concentration between 5 and 15 percent by weight of the formulation in an ointment-based excipient to form the topical formulation of hops extract.

A method for preparing hop extract emulsions is provided. One to two parts hop extract is combined with eight to nine parts diluent oil as an oil mixture. The oil mixture is heated until reaching a viscosity threshold when the heated oil mixture is combined with an emulsifying solution. The emulsifying solution includes an emulsifying agent dispersed in water. Upon combining the oil mixture and emulsifying solution, the emulsifying agent enrobes droplets of the hop extract. The combined oil mixture and emulsifying solution is mixed and homogenized.

A CO.sub.2 extraction system which is capable of being used for extracting compounds, such as oils, from various materials including plant material and botanicals. The extraction system includes a means for supplying CO.sub.2 to a pressure vessel. The pressure vessel is substantially comprised of two distinct portions; a top portion and a bottom portion. The top portion includes a separation zone, while the bottom portion contains a collection zone. Affixed to the bottom portion of the pressure vessel is a drain valve which is capable of allowing extracted material to exit the system. When introducing CO.sub.2 into the extraction system, the CO.sub.2 may be liquefied and further saturated with additional compounds. By maintaining each of the respective top and bottom portions of the pressure vessel at differing temperatures and/or pressures, the various additional compounds may be separated from the CO.sub.2.

A method of purifying a hop raw material includes subjecting the hop raw material to a first processing step to form a hop oil extract substantially free of lipids, waxes, flavonoids, and plant matter, collecting the hop oil extract, and optionally subjecting the hop oil extract to a second processing step to yield a purified hop oil product.