The invention relates to a pro-biofilm coating applied by means of cold atmospheric plasma polymerization of a precursor on a substrate. The coating has a roughness such that it promotes the creation of more than 100% biofilm on the substrate, where the 100% of biofilm is the one as produced on the same substrate being devoid of said pro-biofilm coating. The invention also relates to a method of producing said pro-biofilm coating and a substrate coated with same.

The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non Faradaic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradaic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

A fluidic device according to the present invention includes a base and a lid member. The base and the lid member are configured to form a first flow path, a second flow path, and a third flow path between the base and the lid member, the base and the lid member being bonded to each other, the first flow path communicating with the second flow path through the third flow path. The third flow path has a first accommodation section for detachably accommodating a first cell culture module. The second flow path has a second accommodation section for detachably accommodating a second cell culture module. The first cell culture module contains first cells having a barrier function and a first culture gel. The second cell culture module contains second cells and a second culture gel. The first accommodation section is configured in such a manner that the first cell culture module blocks the third flow path.

This disclosure provides a tissue-engineered transcatheter vein valve and methods of making such a tissue-engineered transcatheter vein valve. Methods of making the valve include casting or molding a polymer into a tubular structure having a first end and a second end, where the first end of the tubular structure is cast or molded around a tubular support structure and where the second end of the tubular structure is cast or molded in the absence of the support structure; everting the polymer at the second end through the support structure; anchoring the second end of the tubular structure to the support structure at a first position and a second position, where the anchored first position and the anchored second position result in commissures, forming leaflets therebetween.

[Problem]

To provide a microorganism deodorizing device capable of sufficiently exhibiting a decomposition-deodorization function while suppressing an increase in manufacturing cost, even in a large-scale device including a large-sized deodorizing tank.

[Solution]

A deodorizing tank 1 of a microorganism deodorizing device 1A forms an airflow passage 20 through which air passes from a chamber unit 3 to an opening portion 19; and the airflow passage 20 is provided with a deodorizing unit 5 in which a foam material 17 is filled, a ventilation resistance layer 4 arranged close to or adjacent to the deodorizing unit 5 and configured to increase ventilation resistance of the air flowing through the airflow passage 20, and a chamber unit 3 arranged close to or adjacent to the deodorizing unit 5 and/or the ventilation resistance layer 4 as a chamber which temporarily stores the air fed to the deodorizing tank 1; and the air is fed to the deodorizing unit 5 in a state of being spread in the chamber unit 3 over a substantially entire surface of the deodorizing unit 5 as a result of that the ventilation resistance of flow of the air flowing through the airflow passage 20 is increased by the ventilation resistance layer 4.

The present disclosure relates to a bioreactor assembly having a housing defining an interior chamber; a lid assembly removably coupled to the housing and enclosing the interior chamber; and a gimbal assembly disposable within the interior chamber. The gimbal assembly includes a cradle configured to hold an organ or organ scaffold and an arm assembly configured to move the cradle between a plurality of positions. The bioreactor assembly also includes an ultrasound imaging unit positioned to capture volumetric and/or spatial data of the organ or organ scaffold.

A jig member for use in the manufacture of a luminal cell structure, in which needle-shaped bodies are arranged in a comb-like form on a thickness-direction surface of a plate-like support having a length direction, wherein a surface which is adjacent to the thickness-direction surface and is along the length direction is formed in a tapered shape toward tips of the needle-shaped bodies; and a jig for use in the manufacture of a luminal cell structure, in which the member is arranged on a circumference of a circle with the tips of the needle-shaped bodies facing in the direction toward the center of the circle.

A cell culture vessel includes a vessel body, support columns, and a stabilizer device. The vessel body defines a cell culture chamber enclosed between a bottom wall and a top wall. The support column is within the cell culture chamber and extends between the top wall and the bottom wall. The stabilizer device covers a width and length of the cell culture chamber and has a column engaging structure that is sized to slidingly engage the support column such that the stabilizer device is movable along the support column as a liquid culture medium is received in the cell culture chamber. The support column guides the stabilizer device along a length of the support column as the stabilizer device rises with rising liquid level in the cell culture chamber during a liquid culture medium filling operation.

A culture container for culturing lymphocytes includes an immobilized surface and a non-immobilized surface, wherein the culture container is formed of a gas permeable film, the immobilized surface and the non-immobilized surface are container inner surfaces facing each other, and anti-CD3 antibodies are immobilized in the immobilized surface at a concentration of 10 to 300 ng/cm.sup.2.

The application provides a system for continuous cell production, comprising: a culture container; and a polymer blended layer arranged on the inner surface of the culture container; wherein, the polymer blended layer is a pH-responsive polymer blended with nylon. Additionally, a method for continuous cell production using the system of the present application is provided.