To provide a substrate of a cell culture container in which cells are appropriately maintained in dents and the cells in the dents are appropriately observed, and a cell culture container.

The substrate of a cell culture container of the present invention has a bottom face and a surface having a dent-formed region having a plurality of dents formed, wherein the average depth of the plurality of dents is 200 .Math.m or more, the formula θ1<90-θ2+sin.sup.-1{sin(θ2)×1.38/n} is satisfied wherein θ1 (deg) is the angle formed by a first tangent line which passes a connecting point of a first curved line corresponding to a first dent and a second curved line corresponding to a second dent and which is in contact with the first curved line, and a base line which passes the connecting point and which is in parallel with the bottom face, and n is the refractive index of the substrate, and the formula θ2<90-θ1+sin.sup.-1{sin(θ1)×1.38/n} is satisfied wherein θ2 (deg) is the angle formed by a second tangent line which passes the connecting point and which is in contact with the second curved line, and the base line.

A method for evaluating an adherent cell includes: obtaining an image of an adherent cell accommodated within a culture container; and determining whether the adherent cell is adhering to the culture container based on at least a profile shape of a contact portion of the adherent cell that is in contact with the culture container, the profile shape being specified from the image.

Disclosed are various embodiments for growing, culturing, monitoring, and analyzing embryoid bodies, fused embryoid bodies, spheroids, organoids, or other multi-cellular bodies using a system of microplates. Different types of microplates are designed to be used during the various stages of growing and culturing of cells to form embryoid bodies, fused embryoid bodies, spheroids, organoids, or other multi-cellular bodies. The different microplates are designed to mate with one another to allow for the transfer of cells from wells in one plate to wells in the other plate. An assay plate includes an array of perfusable units that include a supply well that is in fluid communication with a culture well to allow for an exchange of fluid.

Described herein is a beads-free bioprocessor as an automated and cost-effective T cell processing and manufacturing platform. T cells are a core component in CAR T cell therapies for cancer treatment, but are difficult to manufacture to scale in clinically relevant quantities. The 3D bioprocessor provides an alternative device that is scalable, beads-free, easy-to-use, and cost-effective for using CAR T cell therapy in cancer immunotherapy. Besides CAR T cell application, this platform technology has potential for many other applications such as cancer cell isolation.

The present invention relates to a process for producing alcohols, in which a sugary fluid is introduced into a fermentation reactor (2) to produce a fermentation must enriched in isopropanol, butanol, ethanol and acetone relative to the sugary fluid, the fermentation reactor (2) comprising a biomass produced by a strain belonging to the genus Clostridium which is supported on a solid support (9) comprising a polyurethane foam; and to a fermentation reactor (2) comprising said biomass supported on said solid support (9).

Application of the present invention enables quantification of fractions of candidate pharmaceutical compounds (a parent compound and its metabolites), one excreted to the basolateral (Basal/Basolateral)-side via transporters and by diffusion, one excreted to the lumen (Apical)-side, and one remained in the cells. This enables determination of the total amount of the administered candidate pharmaceutical compounds and the distribution ratio of the fractions. The kinetics of the administered candidate pharmaceutical compounds can be evaluated, thereby enabling in vitro screening of an enormous number of candidate pharmaceutical compounds for drug candidates exhibiting the efficacy. The object of the present invention is to provide an apparatus and method for understanding a total picture of pharmacokinetics in vitro by quantifying a fraction of basolateral (Basal/Basolateral) efflux, a fraction of lumen (Apical)-side excretion, and a fraction remaining in a cell of a drug which has been administered to the cell to determine the distribution ratio of each fraction.

A cell culture tool configured to be produced in a simple facility to obtain a cell mass having a desired shape. The cell culture tool includes a cell culture base layer that contains a cell culture base. The cell culture base layer has a cell adhesive area to which a cell is adherable and a cell adhesion inhibitory area where cell adhesion is inhibited. The cell adhesion inhibitory area includes a modified product of the cell culture base.

The present invention provides methods and systems which accommodate 3-dimensional adipocyte expansion to produce, e.g., mature adipocytes and synthetic adipose tissue with cellular properties of mature adult organisms, including cell size and cytoarchitecture, and the use of such methods and systems for, e.g., in vitro drug screening and/or toxicity assays, disease modeling, and therapeutic applications.

[PROBLEM] It is an object to provide a biological tissue fabrication information generating device that generates biological tissue fabrication information for fabrication of tissue imitative of the structure of the biological tissue of a patient, a biological tissue fabrication system that fabricates biological tissue based on biological tissue fabrication information, and a medical expense calculation system that calculates the medical expense of a patient receiving therapeutic graft of biological tissue. [SOLUTION MEANS] A biological tissue fabrication information generating device characterized in that it comprises structure information storage unit 10 that stores tissue structure information D1 indicating three-dimensional structure of biological tissue of patient Pt, and biological tissue fabrication information generator 11 that generates biological tissue fabrication information D3 in the form of information linking respective locations within biological tissue indicated by tissue structure information D1, and the types of cells that should be arranged at such respective locations, based on tissue structure information D1 and cell type information D4 for determining types of cells at respective locations in biological tissue indicated by tissue structure information D1.

A mixed acrylate-siloxane polymer can be used to create three-dimensional (3D) structures of arbitrary shape via nanolithography. Treatment of such structures with amine (such as diamine) makes them permissive for neuronal cell adhesion and growth without need of additional modification such as poly-lysine (D or L) nor laminin.