An electromagnetic rotary drive includes a single-use device for single use and a reusable device for multiple use. The single-use device includes a rotor housing with a rotor, a connecting tube for a fluid, and a single-use component. The rotor housing has an inlet and an outlet, and the rotor is provided in the rotor housing for rotation about a desired axis of rotation defining an axial direction. The rotor can be magnetically driven without contact, and the connecting tube connects the inlet or the outlet of the rotor housing to the single-use component such that in the operating state the fluid flows through the connecting tube. The reusable device includes a stator, by which the rotor is magnetically driven without contact for rotation about the desired axis of rotation in the operating state.

A system, device and method for electroporation of living cells and the introduction of selected molecules into the cells utilizes a fluidic system where living cells and biologically active molecules flow through a channel that exposes them to electric fields, causing the molecules to be transferred across the cell membrane. The device is structured in a manner that allows precise control of the cells location, motion, and exposure to electric fields within the flow channel device. The method is particularly well suited for the introduction of DNA, RNA, drug compounds, and other biologically active molecules into living cells.

Culture systems and methods of using same. The systems include a housing defining an inner space. The inner space includes a headspace and at least a portion of a reservoir. A surface for immobilizing cells is moveable between the headspace and the reservoir. The systems can be used for coculturing methanotrophs and phototrophs for processing biogas and wastewater, particularly from anaerobic digesters.

A method for mixing a fluid includes: dispensing a first volume of a fluid into a flexible container, the flexible container being at least partially disposed within the chamber of a support housing; repeatedly moving the support housing and the flexible container contained therein so as to mix the first volume of fluid within the flexible container; adding further fluid into the flexible container after moving the support housing to form a second volume of fluid; and manipulating a mixing element within the flexible container so as to mix the second volume of fluid.

An automated bioprocess solution preparation apparatus includes a first mixing chamber containing at least one ingredient for a bioprocess solution, a first port and a second port for a fluid to enter the mixing chamber, and a third port for a liquid bioprocess solution to exit the mixing chamber. The apparatus further includes an array of tubing for fluid flow within the apparatus, a sensor arranged to measure a property of the liquid bioprocess solution exiting the mixing chamber, a first valve associated with the first port, and a second valve associated with the second port. When the property meets or exceeds a threshold value, the apparatus is operable to perform at least one of: closing the first valve to block the fluid from entering the mixing chamber through the first port, and opening the second valve for the fluid to enter the mixing chamber through the second port.

Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol. Biomass feedstock is saccharified in a vessel by operation of a jet mixer, the vessel also containing a fluid medium and a saccharifying agent.

This invention describes a design for a multiwell plate that contains integrated pumps that are used to stir each well of the plate. The device employs microfluidic logic technology to drive each peristaltic pump. This enables the plates to run autonomously, requiring only a static vacuum supply for power. The devices are entirely constructed out of low-cost polymers, with no electronics, and yet contains simple digital logic circuits to control the pumps. A stack of these plates may be run continuously in a standard cell culture incubator, allowing high-throughput culture of organoids.

Disclosed are compositions, methods, and systems for propagating microorganisms for fermentation.

Disclosed is a photosynthetic culture production device including at least one photo-bioreactor chamber having a supply/discharge unit, and including: an aqueous liquid containing a photosynthetic culture; at least one unit for supplying and discharging fluids from the chamber interacting with a management system; at least one light distributor including at least one first wall arranged so as to receive the light at a proximal end, at least one second wall arranged so as to emit at least part of the received light, and a sealed cavity defined by the at least one first wall and the at least one second wall, part of the emitting wall being immersed in the aqueous liquid containing the photosynthetic culture; at least one fluid partially filling the sealed cavity; and a cover, limiting evaporation. The cover has at least one opening, keeping the at least one light distributor stationary in the chamber.

The present invention relates generally to an integrated system, apparatus and method that allows for the continuous culturing of microorganisms under high pressure conditions and at a wide range of temperatures. More specifically, the system is configured to be gas tight and operate under aerobic or anaerobic conditions. The system is also configured to permit periodic sampling of the incubated organisms under such conditions with minimal physical/chemical disturbance inside the reactor and minimal impacts of shear forces on the collected biomass.