A multi-layered microfluidic system featuring tissue chambers for cells in a first layer and a plurality of medium channels for culture medium in a second layer. The tissue chambers fluidly connect to the medium channels such that media flows from the medium channels to the tissue chambers, forming large-scale perfused capillary networks. The capillary networks can undergo angiogenesis and vertical anastomosis. The multi-layered configuration of the system of the present invention allows for flexibility in design.

The invention relates to valve system for controlling a process fluid within a liquid processing system. The valve system comprises a valve arrangement, a pneumatic control system, and a connector unit. When the valve arrangement is connected to the connector unit, two or more valves are formed, such that the pneumatic control system controls an open/close or pressure control mode of the valves. A pump diaphragm system is disclosed, as well as a system for purifying a biological material that comprises the valve system or the pump diaphragm system. Also discloses are methods of using the valve system or the pump diaphragm system in a process for the purification of a biological material.

The purpose of the present invention is to suppress variation in time for cells to be immersed in a peeling liquid when the cells are peeled from culture layers. A multilayer culture vessel 1 has an internal space that is divided by a boundary surface 10 into a culture space 11 on one side and a buffer space 12 on the other side in a direction parallel to a bottom plate 2. The multilayer culture vessel 1 includes at least one intermediate plate 5 that extends in the culture space along the direction parallel to the bottom plate 2 and divides the culture space 11 into a plurality of culture layers 21, wall portions 13 that respectively extend from the bottom plate 2 and the at least one intermediate plate 5 toward a top plate 3 at the boundary surface 10, communication portions 14 that bring the buffer space 12 into communication with the culture layers 21, and a liquid supply/drainage port 6 that is formed in the top plate 3 at a location facing the buffer space 12.

A cell culture device for culturing cells in a closed container kept in an aseptic state includes: a chamber including an opening/closing part capable of being opened and closed; a connection path configured to connect closed containers accommodated in the chamber to each other and each having an inside kept in an aseptic state; a driving part configured to move cells from one closed container to the other closed container via the connection path; and a container attachment/detachment device. After the cells are moved from one closed container to the other closed container, the container attachment/detachment device is configured to remove one closed container from the connection path while maintaining an aseptic state inside the other closed container and the connection path, and connect a new closed container loaded into the chamber to the connection path while maintaining an aseptic state inside the new closed container.

The present invention relates in a first aspect to a plug flow tubular bioreactor having an integral or multi-part tube adapted for cultivation, and, optionally, infection, viral transduction, or transfection of eukaryotic cells. In a further aspect, the present invention relates to a plug flow tubular bioreactor system comprising the plug flow tubular bioreactor according to the present invention. Further, the present invention relates to a method for preparing virus particles, vectors, cells or other molecules including toxic molecules using the plug flow tubular bioreactor or the system according to the present invention. Finally, the present invention relates to the use of a plug flow tubular bioreactor for the preparation of virus particles, vectors including viral vectors, cells including modified cells or other molecules including toxic molecules.

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

A bag assembly for use with a heat exchanger includes a flexible bag having of one or more sheets of polymeric material, the bag having a first end that bounds a first compartment and an opposing second end that bounds a second compartment, a support structure being disposed between the first compartment and the second compartment so that the first compartment is separated and isolated from the second compartment. A first inlet port, a first outlet port, and a first drain port are coupled with the flexible bag so as to communicate with the first compartment. A second inlet port, a second outlet port, and a second drain port are coupled with the flexible bag so as to communicate with the second compartment.

Methods and compositions for making bacteriocins are described in some embodiments herein. In some embodiments, pro-polypeptide comprising the bacteriocins in the desired ratios in cis, and separated by cleavage sited can be produced by a microbial cell comprising a nucleic acid encoding the pro-polypeptide. In some embodiments microfluidic devices and methods for making specified mixtures of antimicrobial peptides and/or bacteriocins are described.

The invention discloses a bioreactor with a vessel defining an inner volume, agitation means and at least one addition tube, wherein a delivery orifice in the addition tube is located within the inner volume and a check valve is arranged in proximity of the delivery orifice for allowing flow of a fluid in the direction from the addition tube into the inner volume of the vessel and blocking flow in the reverse direction.

This cell treatment device is provided with: a factor introduction device 30 for introducing a pluripotent induction factor into cells so as to prepare induction factor-introduced cells; and a reprogramming suspension culture vessel for culturing the induction factor-introduced cells that have been prepared by the factor introduction device 30.