Methods and apparatus that facilitate membrane-perturbing surface interactions for delivering a payload to a variety of cell types without resulting in a substantial loss in cell viability or alteration of endogenous cellular functions. In one example, an intracellular delivery tool comprises a microfluidic device (10) which includes a microfluidic flow channel (12) containing fluid therein and a membrane perturbing surface (22), in fluid communication with the microfluidic flow channel (12), with a plurality of perturbing features disposed thereon. An exemplary intracellular delivery method includes flowing a fluid containing cells therein along a membrane perturbing surface having a plurality of perturbing features disposed thereon, and delivering nanomaterial across a membrane of the cells in the fluid during and after contact between the cells and the membrane perturbing surface.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

In various embodiments, method and devices for delivering large cargos (e.g., organelles, chromosomes, bacteria, and the like) into cells are provided. In certain embodiments method of delivering a large cargo into eukaryotic cells, are provided that involve providing eukaryotic cells disposed on one side of a porous membrane; providing the cargo to be delivered in a solution disposed in a reservoir chamber on the opposite side of the porous membrane; and applying pressure to the reservoir chamber sufficient to pass the cargo through pores comprising said porous membrane wherein said cargo passes through cell membranes and into the cells.

The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.

A neutron ray irradiation target apparatus 100 of the present invention is used to irradiate irradiation targets (seeds, etc.) with a neutron ray generated by a neutron ray irradiation apparatus. The neutron ray irradiation target apparatus 100 has a holding means 70 for holding the irradiation targets. The holding means 70 holds at least one closed container 30 which can accommodate the irradiation targets 20 stacked randomly and three-dimensionally. In the case where the irradiation targets are stacked three-dimensionally and accommodated in the closed container, the irradiation targets overlapping each other are irradiated with the neutron ray in a chain reaction fashion. The neutron ray irradiation target apparatus 100 can be used in a method for irradiating a large amount of irradiation targets (seeds of crops, etc.) with a neutron ray, while reducing a required time, thereby efficiently inducing mutations in the irradiation targets.

In order to culture the pluripotent stem cell which can be variously differentiated, while preventing the cross contamination between the different cells and securing the safety of the products; extending a main transport path 31 outwardly from a stem cell conditioning area 20, the stem cell conditioning area 20 including a treatment room 21 for inducing pluripotent stem cell from a somatic cell or an egg cell, or a treatment room 21 for receiving and conditioning pluripotent stem cell induced in other facilities; branching at least one branched transport path 32 from the main transport path 31; and arranging along each branched transport path 32, a cell culture area 40 including culture rooms 41 to 44 for culturing the stem cell and an analysis room 45 for analyzing the cultured cell, respectively. Preferably, the stem cell conditioning area 20, the cell culture area 40 and a transport area 30 including the main transport path 31 and the branched transport path 32 are provided with respective operator gates 22, (47+48), 33 individually, so as to prohibit coming and going of operator among the areas 20, 40, 30.

Methods, tip assemblies and kits are provided for introducing material into cells. The tip assemblies include an attachment portion, a channel portion, and a constriction that function to reduce fluid pressure as a fluid passes through the constriction portion from the channel portion, whereby the tip assemblies form pores in the membranes of cells and introduce material into the cells. The material includes for example one selected from the group of: an inorganic compound, a drug, a genetic material, a protein, a carbohydrate, a synthetic polymer, and a pharmaceutical composition.

The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. The HTP genomic engineering platform described herein is microbial strain host agnostic and therefore can be implemented across taxa. Furthermore, the disclosed platform can be implemented to modulate or improve any microbial host parameter of interest.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.