The gene transfection system includes an acoustothermal module and a signal generating module; the acoustothermal module includes a piezoelectric substrate, an acoustothermal chip arranged on the piezoelectric substrate and N sound-absorbing vessels arranged on the acoustothermal chip and used for cultivating recipient cells, and N is an integer greater than or equal to 1; the signal generating module is used to output basic frequency signals; the acoustothermal chip is used to convert the basic frequency signal to an acoustic wave signal, establish a temperature gradient field with the acoustic wave signal, and control the temperature of the recipient cell in the sound-absorbing vessels with the temperature gradient field, so that the pores are opened on the membranes of the recipient cell, and the nucleic acids or other substances can go into the cells through these pores.

Provided are a tumour immunogen, a preparation method therefore, and an application. After localised sequential cooling-heating treatment is performed on tumour tissue and/or cells, the tumour tissue and/or cells release a large amount of tumour immunogen heat shock protein 70. The obtained tumour immunogen can activate the body's tumour immune system, to convert immunosuppressive cells into mature dendritic cells, thereby increasing immunogen presentation, and activating tumour immunity.

A method for dynamic evolution and/or adaptation and monitoring of characteristics in living cells is provided, wherein the method may be performed at a microfluidic-enabled cell-culture device comprising pneumatic layer for directing flow of fluid to a plurality of individually addressable wells, and one or more sensors configured to detect data regarding environments inside one or more of the plurality of wells. The method may involve culturing a population of cells in a first well of the plurality of wells, perturbing one or more characteristics of an environment in the first well following the culturing of the population of cells, monitoring one or more characteristics of the population of cells in the first well, and removing all or part of the evolved/adapted population of cells from the first well.

An automated method of T cell scale down processing. The method including: activating T cells by automatically contacting the T cells with one or more activation reagents; transducing the T cells by automatically contacting the T cells with a recombinant viral vector; automatically inoculating T cells; automatically expanding the T cells; optionally, automatically debeading the T cells; and automatically harvesting the T cells. A system for an automated method of T cell scale down processing.

A cell selection and sorting process includes attaching cells of a target cell type to a first set of polymeric beads, washing the chamber through a first filter having a first pore size less than the first bead diameter to retain the first set of polymeric beads and greater than a cell diameter to remove unattached cells, releasing the cells of the target cell type from the first set of polymeric beads, and collecting the cells of the target cell type. A cell modification process includes modifying cells of the target cell type in the chamber. A cell modification system includes a cell modification chamber with entry ports and outlet ports, filters with predetermined pore sized selectably located on the outlet ports, and sets of polymeric beads with predetermined diameters being selected such that the sets of polymeric beads are separable by the filters.

The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. The HTP genomic engineering platform described herein is microbial strain host agnostic and therefore can be implemented across taxa. Furthermore, the disclosed platform can be implemented to modulate or improve any microbial host parameter of interest.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

Disclosed herein are methods, assemblies, systems, kits and devices for introducing molecules or compositions into cells or cell-like bodies. An assembly for introducing molecules in a solution into cells or cell-like bodies comprises a rigid container having a first inner diameter or cross-sectional area at a proximal end thereof and inner and outer walls extending between a distal and proximal end, a plunger insertable into the container at the proximal end, and at least one constriction of only the inner wall proximal to the distal end or at least one constriction of the inner and the outer walls proximal to the distal end, wherein the at least one constriction has a second inner diameter or cross-sectional area that is smaller than the container first inner diameter or cross-sectional area and the plunger is axially movable along the container.

Described herein are methods and systems for cell processing or, more specifically, for introducing various payloads into cells. These methods and systems use a mechanoporation approach in which cells are rapidly compressed and then released to relax while absorbing the payload. More specifically, these methods and systems enable high-throughput mechanoporation with various clogging mitigation features. A cell processing apparatus comprises a shell with an inner shell cylindrical surface, a core with an outer core cylindrical surface, and ridges, supported on and protruding away from one of the inner shell cylindrical surface and the outer core cylindrical surface. The core is disposed inside the shell. The outer core cylindrical surface is concentric with the inner shell cylindrical surface. Each of the ridges forms a ridge gap with the other one of the inner shell cylindrical surface and the outer core cylindrical surface.

A bioprocessing system includes a first fluid assembly having a first fluid assembly line connected to a first port of a first bioreactor vessel though a first bioreactor line of a first bioreactor vessel, a second fluid assembly having a second fluid assembly line connected to a second port of the first bioreactor vessel through a second bioreactor line of the first bioreactor vessel, and an interconnect line providing for fluid communication between the first fluid assembly and the second fluid assembly, and for fluid communication between the second bioreactor line of the first bioreactor vessel and the first bioreactor line of the first bioreactor vessel.