In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

The systems, devices, and methods utilize devices configured to (i) control the loading of each channel layer and/or (ii) prevent formation of bubbles within the channels. A device may include two or more stacked layers. The two or more stacked layers may include a first layer and a second layer. The first entry region diameter of the first layer and the second entry region diameter of the second layer may be different; and/or the first exit region diameter of the first layer and the second exit region diameter of the second layer may be different; and/or one or more of the first channel dimensions (e.g., length and/or width) of the first layer and the one or more of the second channel dimensions (e.g., length and/or width) of the second layer may be different.

In example implementations, an apparatus is provided. The apparatus includes a channel, an energy source, and a transfection chamber. The channel includes an indentation to hold a cell. The energy source is to apply a shockwave to the cell in the channel to porate the cell. The transfection chamber is to store a reagent to be inserted into the cell after the cell is porated.

Methods and apparatus of bioreactors for therapeutic cells manufacturing are provided herein. In some embodiments, a bioreactor includes: an upper bioreactor reservoir configured to perform multiple cell therapy manufacturing process steps including genetic modification and expansion to a plurality of cells disposed therein, wherein the upper bioreactor reservoir includes a plurality of ports for delivering fluids into and out of the upper bioreactor reservoir; a lower bioreactor compartment configured to hold a suspension comprising a molecular species; and a membrane disposed between the lower bioreactor compartment and the upper bioreactor reservoir, wherein the membrane includes a plurality of micro-straws extending through the membrane and into the upper bioreactor reservoir to transfect the plurality of cells with the molecular species.

The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. The HTP genomic engineering platform described herein is microbial strain host agnostic and therefore can be implemented across taxa. Furthermore, the disclosed platform can be implemented to modulate or improve any microbial host parameter of interest.

A module of microfluidic device used for applying shear stress to cells (501) in order to deliver payloads (502) to the cells (501). The module includes cross junction modules (105), serpentine modules and squeezing-relaxing modules (1101). The devices and related methods result in increased payload (502) delivery and reduced loss in cell (501) viability.

In example implementations, an apparatus is provided. The apparatus includes a channel, a thermal inkjet (TIJ) resistor, and a transfection chamber. The TIJ resistor is to apply heat to a cell in the channel to porate the cell. The transfection chamber is to store a reagent to be inserted into the cell after the cell is porated.

Examples disclosed herein relate to single cell transfection with interchangeable reagent. The present disclosure relates generally to a device, method, and system for single cell transfection including a transfection chamber on a transfection chip. The example system may include a fluidic channel located on the transfection chip for guiding a cell towards the transfection chamber, the fluidic channel sized to allow no more than a single cell to arrive at the transfection chamber at a time. The example system may also include a reagent receiver located on the transfection chip guiding received reagent towards the transfection chamber and intersecting with the path of the fluidic channel.

A gel tray for a bacteria transformation lab exercise has a transparent plastic body with four parallel gel channels and four filling ports, one for each channel into which unmodified bacteria and heat-shocked bacteria can be injected by students along with appropriate reaction constituents to demonstrate transformation of the bacteria under visualization.

The present invention discloses a piezoelectric ultrasonic microinjection device based on a flexible hinge mechanism. The device includes: a cover, a flexible hinge mechanism, a base, a screw cap and an end cap fixedly assembled together, the base being provided with a pump interface; and a micropipette fixedly mounted in the base, the screw cap and the end cap and extending outward, the micropipette being in communication with the pump interface; wherein the flexible hinge mechanism comprises a housing, a piezoelectric ceramic package module encapsulated in the housing, a central shaft fixedly mounted with the piezoelectric ceramic package module and the base, and a vibration output shaft extending from the piezoelectric ceramic package module into the central shaft, a plurality of flexible hinge beams being disposed between the central shaft and the housing.