A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

Various embodiments of the present technology provide methods and systems for soil enrichment. The systems may comprise a bioreactor system coupled to an initial treatment system for the cultivation of a live microorganism culture containing organic nutrients on an agriculturally effective scale. The systems may be automated and/or portable for practical applications onto target fields. The live microorganism culture may be delivered onto the soil of the target fields, enriching the soil with the organic nutrients that become bioavailable to crops growing in the soil. The soil enrichment system may provide a sustainable approach to agriculture that may efficiently enhance the natural processes of the native soil of any crop.

Systems and processes of providing novel thermal energy sources for hydrothermal liquefaction (HTL) reactors are described herein. According to various implementations, the systems and processes use concentrated solar thermal energy from a focused high-energy beam to provide sufficient energy for driving the HTL biomass-to-biocrude process. In addition, other implementations convert biowaste, such as municipal biosolids and grease and food waste, to biocrude using anaerobic digesters, and a portion of the biogas generated by the digesters is used to produce the thermal and/or electrical energy used in the HTL reactor for the biomass-to-biocrude process. Furthermore, alternative implementations may include a hybrid system that uses biogas and solar radiation to provide sufficient thermal energy for the HTL reactor.

The disclosure relates to the field of reduction of CO.sub.2 emission, more in particular to CO.sub.2 capture and conversion. The disclosure further relates to the culturing of algae and an apparatus for use thereof. One object of the disclosure is to provide an alternative method for capturing and conversion of CO.sub.2 from a gaseous stream.

Systems and methods for integrating thermochemical processing of biomass and anaerobic digestion are provided. Light oxygenated organic compounds are produced as byproducts of thermochemical biomass processing e.g. by torrefaction and/or pyrolysis, and are converted to methane by anaerobic digestion. Thermochemical processing units may or may not be co-located with the anaerobic digestion units, with co-location providing benefits for e.g. rural agricultural enterprises.

The United States faces significant environmental burden to treat and transport ˜0.61 billion kg of defective tomatoes (culled tomatoes) every year. The present disclosure provides for the treatment and processing of culled tomatoes in microbial-electrochemical systems, using the microbial fuel cell as a model reactor. The fundamental differences between the long-term oxidative behavior of unprocessed culled tomatoes compared to the three readily soluble substrates (dextrose, acetate, and wastewater) are disclosed. AC electrochemical impedance spectroscopy (EIS) analyses indicate the influential impedance contributions of the peel & seed to the cull oxidation. Cyclic voltammetry tests indicate that the indigenous redox-active pigments in the cull influence the faradaic processes involved in the cull oxidation.

Methods and systems for producing a biofuel using genetically modified sulfur-oxidizing and iron-reducing bacteria (SOIRB) are disclosed. In some embodiments, the methods include the following: providing a SOIRB that have been genetically modified to include a particular metabolic pathway to enable them to generate a biofuel; feeding a first source of ferric iron to the SOIRB; feeding sulfur, water, and carbon dioxide to the SOIRB; producing at least the first particular biofuel, a first source of ferrous iron, sulfate, excess ferric iron, and an SOIRB biomass; electrochemically reducing the excess ferric iron to a second source of ferrous iron; providing an iron-oxidizing bacteria that have been genetically modified to include a particular metabolic pathway to enable them to generate a second biofuel; producing at least the second biofuel, a second source of ferric iron, and an IOB biomass; and feeding the second source of ferric iron to the SOIRB.

A device for the removal of volatile organic compounds including at least one first connector arranged at the outlet of a gas stream to be treated. At its top, has a connection extension that links with the bioreactor located in the middle part of the device (having a nutrient solution where organisms that degrade volatile organic compounds can grow). Additionally, there is a dispersion arrangement that couples with an outlet through which the gaseous stream, once treated, is expelled out to the environment. A distribution network of a nutritive solution with microorganisms that degrade volatile organic compounds. The connection extension includes a distributor cone that is arranged in an inverted way and internally emptied and also has a cylindrical extension at its top. The distributor cone allows the airflow to be displaced by an external path into the internal walls of the distributor cone, whose cylindrical extension connects with a second perforated cylinder with multiples drill holes throughout its periphery. The axial axis of the cylindrical extension coincides with the axial axis of the distributor cone. The perforations of this second perforated cylinder are arranged so that the gas output can enter the bioreactor from its bottom.

This invention relates to compositions of matter, methods, modules and instruments for automated mammalian cell growth and mammalian cell transduction followed by nucleic acid-guided nuclease editing in live mammalian cells.

A system and a method for producing biomass from a mixed community of algal species. The method comprises the steps of culturing the mixed community of at least two algal species as biofilms on transparent surfaces having structural features and an optical filter, providing a continuous supply of a culture medium comprising at least 0.5 mol/L aqueous (bi)carbonate and having a pH greater than 9. The method disclosed herein facilitates online monitoring of mixed community productivity by the quantification of oxygen production.