The present invention provides a cell stimulation device capable of uniformly electrically stimulating cells intended to be electrically stimulated, and of preventing medium components from decomposing and depositing. [Solution] The cell stimulation device according to the present invention is intended to stimulate the cells being cultured in a culture medium, and is characterized in that the cell stimulation device includes an electron emission element for feeding electric charges to the culture medium via a gas phase, and an electric charge collecting electrode for collecting the electric charges from the culture medium, the electron emission element includes a lower electrode, a surface electrode, and an intermediate layer disposed between the lower electrode and the surface electrode, and the electric charge collecting electrode is disposed so as to be contactable with the culture medium.

The present invention provides a cell stimulation device capable of uniformly electrically stimulating cells intended to be electrically stimulated, and of preventing medium components from decomposing and depositing. [Solution] The cell stimulation device according to the present invention is intended to stimulate the cells being cultured in a culture medium, and is characterized in that the cell stimulation device includes an electron emission element for feeding electric charges to the culture medium via a gas phase, and an electric charge collecting electrode for collecting the electric charges from the culture medium, the electron emission element includes a lower electrode, a surface electrode, and an intermediate layer disposed between the lower electrode and the surface electrode, and the electric charge collecting electrode is disposed so as to be contactable with the culture medium.

The invention pertains to the field of adaptive cell immunotherapy. It aims at reducing the occurrence of translocations and cell deaths when several specific endonuclease reagents are used altogether to genetically modify primary immune cells at different genetic loci. The method of the invention allows to yield safer immune primary cells harboring several genetic modifications, such as triple or quadruple gene inactivated cells, from populations or sub-populations of cells originating from a single donor or patient, for their subsequent use in therapeutic treatments.

The invention pertains to the field of adaptive cell immunotherapy. It aims at reducing the occurrence of translocations and cell deaths when several specific endonuclease reagents are used altogether to genetically modify primary immune cells at different genetic loci. The method of the invention allows to yield safer immune primary cells harboring several genetic modifications, such as triple or quadruple gene inactivated cells, from populations or sub-populations of cells originating from a single donor or patient, for their subsequent use in therapeutic treatments.

A method is provided to transform progenitor cells, fetal cells, stem cells or tumor cells, e.g., in a microfluidic device, with nucleic acid or protein.

A method is provided to transform progenitor cells, fetal cells, stem cells or tumor cells, e.g., in a microfluidic device, with nucleic acid or protein.

Methods are provided that utilize irradiation to selectively induce release of granules (such as granules containing cytokines or other therapeutically active substances) from platelets. Such irradiation can be performed ex vivo or in vivo, and can be utilized to provide a treated platelet suspension for therapeutic infusion.

Methods are provided that utilize irradiation to selectively induce release of granules (such as granules containing cytokines or other therapeutically active substances) from platelets. Such irradiation can be performed ex vivo or in vivo, and can be utilized to provide a treated platelet suspension for therapeutic infusion.

The invention relates to a method for adjusting an apparatus for treatment using nuclear magnetic resonances. The chronotype of the user is determined. The apparatus is adjusted on the basis of this determination.

The invention relates to a method for adjusting an apparatus for treatment using nuclear magnetic resonances. The chronotype of the user is determined. The apparatus is adjusted on the basis of this determination.