METHOD FOR ADJUSTING AN APPARATUS FOR THE TREATMENT USING NUCLEAR SPIN RESONANCES

Abstract

The invention relates to a method for adjusting an apparatus for treatment using nuclear magnetic resonances. The chronotype of the user is determined. The apparatus is adjusted on the basis of this determination.

Claims

1. A method for adjusting an apparatus for treatment using nuclear magnetic resonances, the method comprising determining the influence of the treatment with nuclear magnetic resonances on a cellular clock of a cell culture or of a user and/or of the chronotype of a user, and setting the apparatus for treatment using nuclear magnetic resonances on a basis thereof.

2. The method as claimed in claim 1, further comprising setting a treatment duration, a treatment interval, a day time of the treatment, a modulation frequency of a field, and/or a field strength of a magnetic field generated by the apparatus for treatment using magnetic fields as a function of the cellular clock.

3. The method as claimed in claim 1, wherein the apparatus for treatment using nuclear magnetic resonances is adjusted so as to achieve a maximum or minimum of a specific protein expression and/or a maximum or minimum phase shift of circadian clock, and/or a synchronization of the circadian clock.

4. The method as claimed in claim 1, wherein the chronotype of the user is determined for setting the apparatus for treatment using nuclear magnetic resonances, and wherein the apparatus is set depending on this chronotype.

5. The method as claimed in claim 4, wherein the circadian clock is determined by temperature measurement, blood pressure measurement, measurement of skin resistance, and/or determination of heart rate.

6. An apparatus for treatment using nuclear magnetic resonances, adjusted by a method as claimed in claim 1.

7. A treatment system comprising an apparatus for treatment using nuclear magnetic resonances as claimed in claim 6, wherein the treatment system comprises means for determining the chronotype of a user, in particular a sensor.

8. The treatment system of claim 7, wherein the sensor is configured for measuring the body temperature, blood pressure, skin resistance, and/or heart rate of a user.

9. The treatment system as claimed in claim 7, wherein the sensor is configured for transmitting data to a control unit of the apparatus, preferably in a wireless manner.

10. The treatment system as claimed in claim 7, wherein the sensor is part of an external unit.

11. A use of nuclear magnetic resonances, comprising altering a circadian clock of cells of a user with the nuclear magnetic resonances.

12. The use according to claim 11, comprising altering the circadian clock for therapeutic and/or cosmetic purposes.

13. The use according to claim 11, comprising altering a hypoxia signaling pathway of cells of the user.

14. The use according to claim 11, comprising altering an amount of free oxygen radicals of cells of the user.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0083] The subject-matter of the invention will now be explained in more detail with reference to the drawings of FIGS. 1 to 5.

[0084] FIG. 1 shows the number of mRNA copies of representative genes from the central feedback loop of the internal clock, plotted over time, representing the oscillation of the internal clock. Shown are the oscillations of cryptochrome1, period1, period2, and clock1. A comparison is shown of oscillations between sham-treated cells and those treated with nuclear magnetic resonances for one hour on four consecutive days at the same time in each case. The phase shift can be clearly seen, it is significant for the period1 and cryptochrome1 genes, (cosinor fit analysis, GraphPad Prism 6.0)

[0085] FIG. 2 shows the changes in mRNA concentrations of hif-1α and hif-3α from the hypoxia signaling pathway between sham-treated cells and those treated with nuclear magnetic resonances for one hour on four consecutive days at the same time (A and E), and the selective synchronization of mRNA oscillations of hif-1α and hif-3α in whole zebrafish larvae according to the same treatment scheme (B and F), while hif-2α remains unaffected both in cells and in whole zebrafish larvae (FIGS. 2, C and D).

[0086] FIG. 3 shows the synchronization of the circadian hif-1 protein oscillations in zebrafish cells achieved by nuclear magnetic resonances, on the one hand after a one-hour treatment that was repeated four times (A), on the other after a single four-hour treatment (B).

[0087] FIG. 4 shows the reduced and dose-dependent amounts of oxidized peroxiredoxin (A), free oxygen radicals (B), and hif-1α protein (C). A 4-hour treatment leads to a reduction in the amount of protein and the amount of free oxygen radicals, respectively, compared to a treatment of only one hour.

[0088] FIG. 5 is a schematic flowchart of a method for adjusting an apparatus for treatment using nuclear magnetic resonances.

[0089] FIG. 6 is a schematic view of an apparatus for treatment using nuclear magnetic resonances.

DETAILED DESCRIPTION OF THE DRAWINGS

[0090] Referring to the graphs of FIGS. 1 through 6, the influence of a treatment using nuclear magnetic resonances on various biological parameters will now be explained.

[0091] In each of FIGS. 1 and 2, the x-axis represents the day time and the y-axis represents the specific number of mRNA copies based on 16 ng of total RNA. In each case, a sham-treated control group is compared to a cell culture that was treated with an MBST® therapy apparatus on four consecutive days for one hour in each case. The treatment was carried out at the same time of day.

[0092] A cell culture of zebrafish fibroblasts was used for this purpose.

[0093] FIG. 1 shows the mRNA oscillations of the genes cryptochrome1, period1, period2, and clock1.

[0094] It can be seen that the circadian mRNA oscillation of the genes period1 and cry1 is shifted by about two hours, compared to the sham-treated control cells. Thus, the phases of these gene oscillations were successfully shifted without the external influence of light. A significant difference in the treatment groups is apparent, which was statistically proven by a cosine curve fit (Graphpad Prism 6).

[0095] FIG. 2 shows the mRNA quantities of the genes hif-1α, hif-2α, and hif-3α over the course of the day.

[0096] It can be seen that the isoforms hif-1α and hif-3α are selectively controlled by nuclear magnetic resonances, on cell level (A and E) as well as in the whole organism (B and F), while hif-2α remains unaffected both in cells and in whole zebrafish larvae.

[0097] FIG. 3 shows the quantities of hif-1α protein of cells over the course of the day. It can be seen that nuclear magnetic resonances cause a synchronization of the circadian rhythm of hif-1α protein expression in both treatment variants (four times one hour [A]—compared to a single treatment over four hours [B]).

[0098] FIG. 4 shows a dependence of the effect of nuclear magnetic resonances on the dose: only a 4-hour single treatment shows a significant reduction in the quantities of oxidized Prx (A), free oxygen radicals (B), and hif-1α (C), in contrast to a single treatment of only one hour.

[0099] FIG. 5 is a flowchart of a method for adjusting an apparatus for treatment using nuclear magnetic resonances according to an exemplary embodiment of the invention.

[0100] First, the phase of the internal clock of the patient is determined through skin resistance, blood pressure, body temperature, and/or heart rate, i.e. the individual chronotype is determined. On this basis, the treatment duration, time interval of a sequence of treatments, day time window for treatment, and/or modulation frequency f.sub.m of the apparatus for treatment using nuclear magnetic resonances are set as a function of the chronotype.

[0101] Based on this setting, a plurality of treatments are executed. These may be therapeutic treatments or else cosmetic treatments.

[0102] FIG. 6 is a schematic view of a system 6 according to the invention for treating a user with nuclear magnetic resonances. The system 6 comprises an apparatus 1 for generating nuclear magnetic resonances in a tissue to be treated.

[0103] The apparatus 1, shown schematically here, comprises coils 2 and 3 which face each other in a Helmholtz arrangement and which are used to generate a homogeneous magnetic field that extends along the direction of the indicated x-axis.

[0104] In order to generate a nuclear magnetic resonance, an alternating magnetic field is irradiated by coil 4, perpendicular to the homogeneous magnetic field generated by coils 2 and 3.

[0105] Its frequency is tuned such that the Larmor condition is achieved.

[0106] Furthermore, the homogeneous field generated by coils 2 and 3 is composed of a basic magnitude and a modulation magnitude which is modulated by modulation frequency f.sub.m, for example in a rectangular shape.

[0107] Due to the modulation, the resonance condition is generated at least once during each cycle within the treatment volume.

[0108] The apparatus 1 comprises a memory which stores user data, data on the duration of treatment, the treatment interval and the modulation frequency f.sub.m.

[0109] The time window of the treatment, treatment duration, treatment interval, and/or modulation frequency are performed as a function of the chronotype of the person to be treated.

[0110] According to the invention, the system 6 comprises means for determining the chronotype of the user.

[0111] In the present case, this is a sensor 5 which forms part of an external unit that can be attached to the user and transfers data to a control unit of the apparatus 1, preferably in a wireless manner. This may also be achieved via the Internet, for example.

[0112] By way of example, the sensor 5 is able to measure heart rate, body temperature, or skin resistance over a period of at least 24 hours. On the basis of the measured values, the chronotype of the respective user is determined.

[0113] Depending on the phase of the circadian clock of the user, a treatment profile with optimized treatment parameters can now be established, preferably automatically by the control unit of the apparatus.

[0114] This applies in particular to the day time at which the treatment is to be performed.

[0115] The apparatus 1 preferably comprises an apparatus control unit which also includes the memory with the aforementioned data, and the control unit of the apparatus calculates optimized treatment parameters preferably automatically, for example on the basis of a calculation program or on the basis of a database, and stores them in the memory in association with the respective user data.

[0116] The setting of the parameters of the treatment apparatus on the basis thereof allows to improve, in a simple way, the effectiveness of the treatment with nuclear magnetic resonances.

[0117] A microcomputer chip card is used to enter the stored treatment sequences into the control unit, via a chip card reader, which ensures the targeted and proper execution of the MBST® nuclear magnetic resonance treatment.

[0118] The sequences on the microcomputer chip card, the treatment procedure, and the treatment time can be selectively modified, verified and readjusted in order to initiate the resonance effects during the treatment duration.

[0119] This is crucial for the highly significant increase in ATP and the hypoxia level of the cells and for triggering the resynchronization of the circadian clock of the cells.

[0120] Studies have shown that the defense of the immune system can be enhanced and that degenerated cell functions can be eliminated thereby.