A device and method are disclosed for regulated storage capacitor charging to high voltage. The device comprises an AC source configured to output an AC voltage, a voltage multiplier that constitutes a charging unit and a control unit. The control unit is configured to constantly sense the voltage on the storage capacitor and upon detecting that a predefined maximum charging voltage has been reached to react in at least one of the following ways: stop charging the storage capacitor, and closing an output switch so as to discharge of the storage capacitor through some load. The capacitance of each capacitor in the charging unit is substantially smaller than that of the storage capacitor so as achieve accurate maximum charging voltage as well as limited charging current.

Feeder cell products are needed to enhance growth of cells that are being cultured. The current process produces feeder cells by selecting a source for blood material containing mononuclear cells (MNCs) and other blood components, combining blood material from a plurality of donors, isolating the MNCs from the other blood components so as to form isolated heterogenous MNCs. Afterwards, the isolated heterogeneous MNCs is irradiated to produce the feeder cells.

Feeder cell products are needed to enhance growth of cells that are being cultured. The current process produces feeder cells by selecting a source for blood material containing mononuclear cells (MNCs) and other blood components, combining blood material from a plurality of donors, isolating the MNCs from the other blood components so as to form isolated heterogenous MNCs. Afterwards, the isolated heterogeneous MNCs is irradiated to produce the feeder cells.

A method and a device that enable inactivation of a virus even if the nature and the like of the virus is unknown. The method for inactivating a virus involves preparing a laser that emits laser light having a specific wavelength, and irradiating a virus with the laser light to inactivate the virus.

A method and a device that enable inactivation of a virus even if the nature and the like of the virus is unknown. The method for inactivating a virus involves preparing a laser that emits laser light having a specific wavelength, and irradiating a virus with the laser light to inactivate the virus.

The invention relates to new methods of moving helicases past spacers on polynucleotides and controlling the loading of helicases on polynucleotides. The invention also relates to new methods of characterising target polynucleotides using helicases.

A composition according to an embodiment includes a first population of transformed mammalian cells with a transforming growth factor beta (TGF-β), the first population having a TGF-β expression level of 0.65 ng/10.sup.5 cells/24 hours or more, and a second population of mammalian cells which are not transformed with the transforming growth factor beta, the second population having an expression level of a thrombospondin 1 (TSP-1) expression level of 31 ng/10.sup.5 cells/24 hours or more.

A process for separating sperm cells from a chemical compound by electrophoresis comprising subjecting the sperm cells to an electric potential between a cathode and an anode such that the sperm cells are separated from the chemical compound, and related methods including methods of using said sperm cells in intrauterine insemination.

A process for separating sperm cells from a chemical compound by electrophoresis comprising subjecting the sperm cells to an electric potential between a cathode and an anode such that the sperm cells are separated from the chemical compound, and related methods including methods of using said sperm cells in intrauterine insemination.

The present invention provides systems, methods, and devices for electroporation-based therapies (EBTs). Embodiments provide patient-specific treatment protocols derived by the numerical modeling of 3D reconstructions of target tissue from images taken of the tissue, and optionally accounting for one or more of physical constraints or dynamic tissue properties. The present invention further relates to systems, methods, and devices for delivering bipolar electric pulses for irreversible electroporation exhibiting reduced or no damage to tissue typically associated with an EBT-induced excessive charge delivered to the tissue.