The present invention relates to a nanosatellite-substrate complex capable of regulating stem cell adhesion and differentiation, and a method for preparing the same. Moreover, the present invention relates to a method of regulating stem cell adhesion and differentiation by applying a magnetic field to the nanosatellite-substrate complex.

The present invention relates to a nanosatellite-substrate complex capable of regulating stem cell adhesion and differentiation, and a method for preparing the same. Moreover, the present invention relates to a method of regulating stem cell adhesion and differentiation by applying a magnetic field to the nanosatellite-substrate complex.

The present invention relates to a method of preparing a live attenuated vaccine by irradiation and a live attenuated vaccine composition prepared by the same, and more particularly, a method of preparing a live attenuated vaccine by irradiation including irradiating a pathogenic microorganism with a dose of 0.5 to 2 kGy of radiation per single radiation six to fifteen times; and a live attenuated vaccine composition including a pathogenic microorganism attenuated to not be revertant to a wild type by generation of at least one mutation of nucleotide insertion and nucleotide deletion by irradiation.

The present disclosure provides a composite material. The composite material comprises nanoparticles and a flexible substrate, the nanoparticles comprise one or more of carbon nanotubes, graphene, gold nanoparticles, and polydopamine nanoparticles, the flexible substrate comprises one or more of thermosetting plastics such as polydimethylsiloxane and a hydrogel, and the mass percentage of the nanoparticles in the composite material is 0 to 60‰. The composite material of the present disclosure is easy to prepare, has extremely strong photothermal conversion performance, and does not change the smooth surface of an original topological structure. Meanwhile, the composite material has universality and versatility for different cells, the delivery efficiency is close to 100%, and modified cells may be efficiently and non-destructively released and harvested by means of traditional trysinization, and the harvesting efficiency is 90% or more.

The present disclosure provides a composite material. The composite material comprises nanoparticles and a flexible substrate, the nanoparticles comprise one or more of carbon nanotubes, graphene, gold nanoparticles, and polydopamine nanoparticles, the flexible substrate comprises one or more of thermosetting plastics such as polydimethylsiloxane and a hydrogel, and the mass percentage of the nanoparticles in the composite material is 0 to 60‰. The composite material of the present disclosure is easy to prepare, has extremely strong photothermal conversion performance, and does not change the smooth surface of an original topological structure. Meanwhile, the composite material has universality and versatility for different cells, the delivery efficiency is close to 100%, and modified cells may be efficiently and non-destructively released and harvested by means of traditional trysinization, and the harvesting efficiency is 90% or more.

A method for cellular separation, including: combining sperm with magnetic particles comprising a negative zeta potential charge to form an admixture, each magnetic particle being no greater than 1,000 nm; binding a subpopulation of said sperm to said magnetic particles through an electrical charge interaction to provide a bound subpopulation; and magnetically separating said bound subpopulation from unbound sperm.

A method for cellular separation, including: combining sperm with magnetic particles comprising a negative zeta potential charge to form an admixture, each magnetic particle being no greater than 1,000 nm; binding a subpopulation of said sperm to said magnetic particles through an electrical charge interaction to provide a bound subpopulation; and magnetically separating said bound subpopulation from unbound sperm.

An apparatus comprising an actuator for moving an actuator rod and a bioreactor vessel is disclosed herein. The bioreactor vessel comprises a container for holding a liquid, a mounting for mounting a tissue sample in the container, and, an actuator coupling to enable the actuator rod to be connected for applying mechanical force to the tissue sample. The apparatus also comprises a seat, fixed with respect to the actuator and configured for locating the reactor vessel in a location selected so that the actuator can be connected for applying said force via the actuator coupling. The reactor vessel is removable from the apparatus.

An apparatus comprising an actuator for moving an actuator rod and a bioreactor vessel is disclosed herein. The bioreactor vessel comprises a container for holding a liquid, a mounting for mounting a tissue sample in the container, and, an actuator coupling to enable the actuator rod to be connected for applying mechanical force to the tissue sample. The apparatus also comprises a seat, fixed with respect to the actuator and configured for locating the reactor vessel in a location selected so that the actuator can be connected for applying said force via the actuator coupling. The reactor vessel is removable from the apparatus.

In accordance with the present disclosure, exposure of a sample to one or more electric pulses via capacitive coupling is described. In certain embodiments, the sample may be a biological sample to be treated or modified using the pulsed electric fields. In certain embodiments, the electric pulses may be delivered to a load using capacitive coupling. In other embodiments, the electric pulses may be bipolar pulses.