The present invention relates to a method for direct transdifferentiation into neurons using metal nanoparticles magnetized by an electromagnetic field, and to a cell therapeutic agent for the treatment of cerebral nerve diseases, comprising neurons differentiated by the method. In the present invention, it was specifically verified that the direct transdifferentiation efficiency into neurons can be remarkably improved through the above method and the symptoms of cerebral nerve diseases, such as a stroke, can be effectively alleviated. Therefore, in the treatment of degenerative cerebral nerve diseases, the target therapy is expected to be implemented through a more fundamental approach.

The present invention provides a method for inducing differentiation of neural stem cells. The present invention provides optimized differentiation conditions of neural stem cells into neurons using a patterned hydrogel.

The technology described herein relates, at least in part, to compositions comprising and methods for isolating and enriching natural IgM-producing phagocytic B (NIMPAB) cells and methods of producing IgM antibodies using such cells, as well as uses of the antibodies produced by the methods for the prevention and treatment of diseases wherein immunotherapy with such natural IgM antibodies and their derivatives can be useful.

In one aspect, the present disclosure provides a method for self-assembly of magnetic building blocks, including distributing a plurality of building blocks in a liquid medium, each of the plurality of building blocks having a plurality of stable radicals, establishing a magnetic field interacting with at least a portion of the plurality of building blocks, guiding with the magnetic field the portion of the plurality of building blocks from a first location in the liquid medium to a second location in the liquid medium, assembling into a first construct the portion of the plurality of building blocks proximate the second location, and treating the first construct with at least one antioxidant to neutralize at least in part the plurality of stable radicals.

Provided subject matter relates to tissue engineering. More specifically provided are kits, devices and methods for in situ repair and regeneration of guided and functional growth of cells and cell components by providing into the injury site biomaterial solution including the cell(s), magnetic particles and solidifying the biomaterial while applying the magnetic field.

According to one aspect of the present invention, there is provided a method for preparing customized pluripotent stem cells and induced pluripotent stem cells thereby. According to this method, a large number of induced pluripotent stem cells can be obtained in high yield. In addition, the induced pluripotent stem cells produced thereby can be used in various fields such as cell therapeutic agents and cosmetic composition.

A method of manufacturing and/or using a scaffold assembly for stem cell culture and tissue engineering applications is disclosed. The scaffold at least partially mimics a native biological environment by providing biochemical, topographical, mechanical and electrical cues by using an electroactive material. The assembly includes at least one layer of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application. A method of cell tissue engineering and/or stem cell differentiation that uses the assembly seeded with a sample of cells suspended in cell culture media, incubates and applies voltage to one or more layers, and thus produces cells and/or a tissue construct. In another aspect, the invention provides a method of manufacturing the assembly including the steps of providing a first pre-electroded substrate surface; electrospinning a first substantially aligned polymer fiber layer onto the first surface; providing a second pre-electroded substrate surface; electrospinning a second substantially aligned polymer fiber layer onto the second surface; and, retaining together the layered surfaces with a clamp and/or an adhesive compound.

The invention provides a microscaffold comprising a porous particle, which particle: (a) comprises a three dimensional network of fibres, which fibres comprise a polymer, and (b) has a particle size of less than or equal to 2000 m. Further provided is a composition, which composition comprises a microscaffold of the invention or a plurality of microscaffolds of the invention. The invention also provides a multi-well assay plate comprising: a plurality of sample wells, and a composition of the invention in at least one of the sample wells. The microscaffold, composition or multi-well plate may be used for regenerative medicine, tissue engineering, screening compounds for biological use or drug screening. The microscaffold may further comprise a magnetic material, and the invention additionally provides a method of manipulating one or more such microscaffolds. The method comprises exposing a composition that comprises one or more such microscaffolds to a magnetic field of a magnet, and thereby causing the one or more microscaffolds in the composition to be attracted to said magnet by magnetic attraction.

Provided herein are systems and methods for cardiomyocyte pacing of cultured cells, including cardiomyoctyes. In particular, provided herein are systems and methods employing electrode arrays with multiwell culture devices that provide electrical stimulation to cells cultured in individual wells of the devices.

The present invention provides methods of differentiating mesenchymal stem cells or adult stem cells into nerve cells by treating the mesenchymal stem cells or the adult stem cells with an electromagnetic field having a high intensity of 100 to 1,500 mT and a low frequency of 0.01 to 100 Hz. These methods also provide injecting the mesenchymal stem cells or adult stem cells into a subject prior to treating the mesenchymal stem cells or adult stem cells with an electromagnetic field having a high intensity of 100 to 1,500 mT and a low frequency of 0.01 to 100 Hz.