Provided are compositions including a direct interface between a biological tissue and a crystalline material, wherein the crystalline material has a crystallization temperature that exceeds the temperature at which the biological tissue incurs thermal damage. Also provided are methods for producing said compositions.

The present disclosure describes hydrogels which are micropatterned with a network of wells for cell culture. In a preferred embodiment, the micropatterned hydrogels are embedded with a nanomaterial. Further described are methods of forming the micropatterned hydrogels and methods of culturing cells in the micropatterned hydrogels. The hydrogels can be natural or synthetic.

Various embodiments disclosed relate to conductive graphene matrix-encapsulated cells. A matrix-encapsulated cell includes an encapsulating polymer matrix including a biopolymer and graphene. The matrix-encapsulated cell also includes one or more of the cells encapsulated within the encapsulating polymer, wherein the graphene directly contacts at least some of the cells. The matrix encapsulating the one or more cells is electrically conductive.

The anatomical model of a nasal cavity, such as a human nasal cavity, for in-vitro inhalation studies such as toxicological screening, intranasal drug delivery studies, and neurophysiological studies. The model includes a model body including separable upper and lower model portions together defining the nasal cavity and including fluidic channels therein that define an olfactory region of the upper model portion, and a nasal passage defined in the lower model portion. A biocompatible porous membrane is positioned between the upper and lower model portions, and the biocompatible membrane is configured for culturing olfactory epithelium cells thereon. An artificial mucous layer coats a surface of the nasal cavity and is configured to collect particles passing through the nasal cavity.

The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.

Described is a composite material composed of an atomically thin (single layer) amorphous carbon disposed on top of a substrate (metal, glass, oxides) and methods of growing and differentiating stem cells.

In accordance with the method of the present invention, 3D tissue-derived scaffolding materials are made in various formats, including but not limited to hydrogel, sponge, fibers, microspheres, and films, all of which function to better preserve natural extracellular matrix molecules and to mimic the natural tissue environment, thereby effectively guiding tissue regeneration. The method involves incorporating a homogenized tissue-derived suspension into a polymeric solution of synthetic, natural, or hybrid polymers to prepare tissue-derived scaffolds in the aforementioned formats. Such tissue-derived scaffolds and scaffolding materials have a variety of utilities, including: the creation of 3D tissue models such as skin, bone, liver, pancreas, lung, and so on; facilitation of studies on cell-matrix interactions; and the fabrication of implantable scaffolding materials for guided tissue formation in vivo. The tissue-derived scaffolds and scaffolding materials made in accordance with the present invention also provide the opportunity to correlate the functions of extracellular matrix with tissue regeneration and cancer metastasis, for example.

A method for transporting cells. The cells are mixed with a material and then directly transported at ambient temperature; or the cells are mixed with a material, subject to three-dimensional culture, and then transported at ambient temperature. During the transportation, the cells keep a high survival rate of up to 80% or more; and after the transportation, the cell/material mixture is directly used, or the cells is isolated for use.

A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

Disclosed here is a three-dimensional electronic scaffold, comprising a porous scaffold and a plurality of micro-strain gauges distributed spatially inside the porous scaffold, wherein the micro-strain gauges are adapted to detect contraction force. Also disclosed is a method comprising detecting and mapping intra-tissue cardiac contraction force of one or more cardiac cells or tissues disposed in a three-dimensional electronic scaffold, wherein the three-dimensional electronic scaffold comprises a porous scaffold and a plurality of micro-strain gauges distributed spatially inside the porous scaffold and in contact with the cardiac cells or tissues, and wherein the micro-strain gauges are adapted to detect contraction force of the cardiac cells or tissues.