The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

The various embodiments of the disclosure relate generally to processes, methods, and systems for generating functional B cells ex vivo. It is particularly useful for ex vivo generation of antigen-specific germinal-center (GC) like B cells that are capable of efficient B cell expansion, immunoglobulin (Ig) class switching/class switching recombination (CSR), expression of germinal B cell phenotypes, antibody secretion, and somatic hypermutation (SHM) and resulting affinity maturation center phenotypes.

A device and method for cultivating cells are provided, wherein a cell cultivating layer is formed on a surface of a substrate, and a temperature-responsive layer having a plurality of temperature-responsive polymer with magnetic objects is formed on a surface of the cell cultivating layer. When a controlling characteristic exerted on the temperature-responsive layer is varied, a plurality of cells can be adhered on the cell cultivating layer or detached therefrom. When the device is utilized, a temperature control step is operated to control environmental temperature at a first temperature for inducing temperature-responsive polymers becoming hydrophobic whereby the plurality of cells are adhered on the cell cultivating layer. In addition, the alternating magnetic field is utilized to rise temperature of the temperature-responsive polymers so that the plurality of cells can be kept being adhered on the cell cultivating layer at a second temperature lower than the first temperature.

The present invention is directed to methods for forming microparticles useful for cell seeding and for conjugating protein to the surface of the microparticles. The method comprises co-injecting an organic solution of PLGA or other polymer with an aqueous solution into a flow focusing tube.

Provided is a method for obtaining female germline stem cells from follicular aspirates.

Provided herein are compositions and methods for generating visible light photopolymerized hydrogels to support cell viability, expansion, and encapsulation.

Disclosed are microbeads for cell culture and a method of monitoring cell culture using the same. More particularly, each of the microbeads for cell culture according to an embodiment of the present invention include a core and a surface modification layer formed on a surface of the core. By using the method of monitoring cell culture with the microbeads for cell culture according to an embodiment of the present invention, cell culture may be carried out in highly scaled-up dimension and easily monitored.

The present invention relates to a cell culture support comprising a substrate and a polymeric blend layer bound to the substrate. The polymeric blend layer comprises at least one thermoresponsive polymer and at least one coupling agent. The coupling agent is a non-protein coupling agent that has functional thiol, ester, epoxy, or aldehyde groups. The cell culture support further includes cells supported by the polymeric blend layer, wherein the thermoresponsive polymer provides for temperature induced detachment of the cells and/or cell sheets.

A polypeptide copolymer, a preparation method thereof, a porous fibrous scaffold including the same, and a method for nerve regeneration or growth are disclosed. The polypeptide copolymer comprises: a glutamate unit; and a glutamic acid unit, wherein a ratio of a content of the glutamic acid unit to a content of the glutamate unit is in a range from 10:90 to 90:10.

A composition for producing a fiber, containing (A) a polymer compound containing a unit structure represented by the formula (1) and a unit structure represented by the formula (2), (B) a crosslinking agent, (C) an acid compound, and (D) a solvent ##STR00001##
wherein each symbol in the formulas (1) and (2) is as described in the DESCRIPTION.