A chip device for nucleic acid detection in a sample, includes a substrate and piston-type containers. The piston-type containers are communicated with each other by means of microfluidic channels; the chip device for nucleic acid detection is further provided with piston valves each located between containers for controlling the fluid communication between the containers communicated with each other; the chip device for nucleic acid detection comprises: a sample receiving module; a sample lysis module; a nucleic acid extraction module, optionally, the nucleic acid extraction module comprising a nucleic acid binding unit, a nucleic acid cleaning unit and a nucleic acid elution unit; and a nucleic acid amplification module. The nucleic acid amplification module comprises multiple amplification area units and an optional pre-amplification area unit.

A nucleic acid construct for AIDS gene therapy. The nucleic acid construct comprises multiple polynucleotides encoding single chain variable region fragments of an anti-AIDS neutralizing antibody, and a polynucleotide encoding an immunoglobulin Fc fragment; the single chain variable region fragments of the anti-AIDS neutralizing antibody comprise a single chain variable region fragment capable of specifically binding to HIV, and a single chain variable region fragment capable of specifically binding to a CD4 receptor. The construct can be used for gene therapy of AIDS caused by HIV infection, and the construct can be used for expressing a bispecific/polyspecific neutralizing antibody having broad-spectrum and high-efficiency neutralizing activity in vitro and in vivo and can be used for clinical research and new drug development of AIDS gene therapy drugs delivered by recombinant viral or non-viral vectors.

The present invention relates to the field of CAdV vector vaccines, and especially to promoters suitable to express target antigens from such vector vaccines. Disclosed and claimed are recombinant canine adenoviruses, methods of making them, uses for them (including in immunological, immunogenic, vaccine or therapeutic compositions, or, as a vector for cloning, replicating or expressing DNA and methods of using the compositions and vector), expression products from them, and uses for the expression products. Additionally, disclosed and claimed are truncated EHV4 promoters, expression cassettes containing the promoters, and recombinant viruses and plasmids containing the promoters or expression cassettes.