A fermentation process includes contacting a starch hydrolysate with a glucoamylase with agitation. And allowing for settling to form a multi-phase solution. A first phase of the multi-phase solution includes a saccharide component comprising about 30 wt % to about 80 wt % (e.g., 30 wt % to 70 wt %, 30 wt % to 60 wt %) based on a total carbohydrate present. The method further includes draining the first phase to isolate the first phase from a second phase to form a fermentation broth comprising a first portion of the first phase. The method further includes fermenting the fermentation broth until a concentration of glucose in the fermentation broth is 40 g/L or less. The method includes adding a second portion of the first phase to the fermentation broth to maintain a concentration of glucose in a range of from about 1 g/L to about 20 g/L in the fermentation broth.

A fermentation process includes contacting a starch hydrolysate with a glucoamylase with agitation. And allowing for settling to form a multi-phase solution. A first phase of the multi-phase solution includes a saccharide component comprising about 30 wt % to about 80 wt % (e.g., 30 wt % to 70 wt %, 30 wt % to 60 wt %) based on a total carbohydrate present. The method further includes draining the first phase to isolate the first phase from a second phase to form a fermentation broth comprising a first portion of the first phase. The method further includes fermenting the fermentation broth until a concentration of glucose in the fermentation broth is 40 g/L or less. The method includes adding a second portion of the first phase to the fermentation broth to maintain a concentration of glucose in a range of from about 1 g/L to about 20 g/L in the fermentation broth.

Increasing space-time-yield, carbon-conversion-efficiency and carbon substrate flexibility in the production of fine chemicals The inventors of the current invention have found a surprising positive effect of increased cAMP levels and/or manipulating the PTS system on the space-time-yield, carbon-conversion-efficiency and carbon substrate flexibility of fine chemical production of a host organism. This was achieved by de-regulating adenylate cyclase cyaa by deleting the C-terminal regulatory region leading to increased cAMP levels or deleting the Crr protein activity (carbohydrate repression resistance) which regulates the carbohydrate utilization system. Both lead to increased 2-fucosyllactoe and 6-sialyllactose production (human milk oligosaccharides) and increase carbohydrate usage.

The invention discloses the application of a β-N-acetylhexosaminidase (HaHex74) from Haloferula sp. in the synthesis of human milk oligosaccharides. The invention provides the use of HaHex74 protein or related biological materials thereof in any one of the following: synthesizing human milk oligosaccharides; synthesizing Lacto-N-triose II and/or Lacto-N-neotetraose; the HaHex74 protein having the amino acid sequence shown in SEQ ID No. 2 is derived from Haloferula sp. The β-N-acetylhexosaminidase HaHex74 disclosed by the invention possesses high-level expression, excellent hydrolysis properties and transglycosylation activity, which may make it potentially useful in the production of human milk oligosaccharides.

The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable xylanase that is resistance to inhibition by metal ions in the liquefying starch-containing material is present and/or added during liquefaction.

The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable xylanase that is resistance to inhibition by metal ions in the liquefying starch-containing material is present and/or added during liquefaction.

The present invention relates to the field of recombinant production of biological molecules in host cells. More particularly it relates to a method for recombinant production of human milk oligosaccharides (HMO) using a genetically modified cell expressing a protein of the major facilitator superfamily (MFS).

The present invention relates to the field of recombinant production of biological molecules in host cells. More particularly it relates to a method for recombinant production of human milk oligosaccharides (HMO) using a genetically modified cell expressing a protein of the major facilitator superfamily (MFS).

The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of fermentation of metabolically engineered host cells. The present invention describes a method of making sialylated oligosaccharide by fermentation with a genetically modified cell, as well as to the genetically modified cell used in the method. The genetically modified cell comprises at least one nucleic acid sequence coding for an enzyme involved in sialylated oligosaccharide synthesis and at least one nucleic acid expressing a membrane protein.

A two-step method for activating a cellulosic feedstock is described. The feedstock is subjected to a first high temperature activation step at a temperature greater than 190° C. and a second activation step at a lower temperature under alkali conditions. Also described are methods and compositions for the enzymatic hydrolysis of activated cellulose using one or more cellulase enzymes, a surfactant and polyaspartic acid. Also described are products of the methods.