The disclosure relates to methods and compositions for the production of fructans using sucrose:sucrose 1-fructosyl-transferase (1-SST), fructan:fructan 1-fructosyltransferase (1-FFT), and/or sucrose fructan-6-fructosyltransferase (6-SFT) enzymes.

Unique carbon dioxide or lignocellulosic substrate is prepared and used to produce biosurfactants, based on different types of microorganism fermenting strains, using carbon dioxide or lignocellulose-based raw materials as the primary feedstock, subsequently utilizing a fermentation process to synthesize different structures of biosurfactants. This is a two-phase reaction where phase-one creates the feedstock for the phase-two reactions. The fermentation broth resulting from the phase-two reaction is the crude biosurfactant; it uses glycolipid or lipopeptide biosurfactant as the main component. The broth is then refined by filtration, then concentrated, and further purified to obtain the pure biosurfactant material. The biosurfactant of the present disclosure can be applied to industries such as petroleum, food or agriculture, daily chemicals, industrial chemicals, environmental protection, and medicine.

Unique carbon dioxide or lignocellulosic substrate is prepared and used to produce biosurfactants, based on different types of microorganism fermenting strains, using carbon dioxide or lignocellulose-based raw materials as the primary feedstock, subsequently utilizing a fermentation process to synthesize different structures of biosurfactants. This is a two-phase reaction where phase-one creates the feedstock for the phase-two reactions. The fermentation broth resulting from the phase-two reaction is the crude biosurfactant; it uses glycolipid or lipopeptide biosurfactant as the main component. The broth is then refined by filtration, then concentrated, and further purified to obtain the pure biosurfactant material. The biosurfactant of the present disclosure can be applied to industries such as petroleum, food or agriculture, daily chemicals, industrial chemicals, environmental protection, and medicine.

Disclosed is a method for the manufacture of a spray-dried powder consisting essentially of 3-fucosyllactose, the spray-dried powder, its use for the manufacture of nutritional compositions, and nutritional compositions containing the spray-dried powder.

Disclosed is a method for the manufacture of a spray-dried powder consisting essentially of 3-fucosyllactose, the spray-dried powder, its use for the manufacture of nutritional compositions, and nutritional compositions containing the spray-dried powder.

The application relates to an enzyme composition, a process for the preparation thereof and the use of the enzyme composition in enzymatic hydrolysis.

A Trichoderma reesei mutant strain has a function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2 is reduced. A method of producing a protein includes a step of cultivating the Tricho-derma reesei mutant strain, and a method of producing a cellulase includes a step of cultivating the Trichoderma reesei mutant strain.

A Trichoderma reesei mutant strain has a function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2 is reduced. A method of producing a protein includes a step of cultivating the Tricho-derma reesei mutant strain, and a method of producing a cellulase includes a step of cultivating the Trichoderma reesei mutant strain.

Provided is a means for preparing a free ceramide (Cer) from a glycosyl inositol phospho ceramide (GIPC). Specifically provided is a composition for digesting a glycosyl inositol phospho ceramide (GIPC) or a glucosylceramide for producing a free ceramide, the composition comprising a mushroom self-digestion liquid or purified product thereof.

Provided is a means for preparing a free ceramide (Cer) from a glycosyl inositol phospho ceramide (GIPC). Specifically provided is a composition for digesting a glycosyl inositol phospho ceramide (GIPC) or a glucosylceramide for producing a free ceramide, the composition comprising a mushroom self-digestion liquid or purified product thereof.