Primary leachate is used as a plant growth stimulant. A fermentation medium is fermented with a microbial culture in a bioreactor to produce a primary leachate comprising microorganisms derived from the microbial culture and/or naturally occurring microorganisms. The primary leachate is isolated from the bioreactor, diluted with water, and used to irrigate plants to reduce bacterial diversity and stimulate beneficial microorganisms in the rhizosphere around the plants. The fermentation medium may be organic waste, preferably food waste. A secondary leachate may also be used as a plant growth stimulant. The primary leachate is used to culture black soldier fly larvae with a substrate in a secondary processing bioreactor under suboptimal culture conditions, thereby producing secondary leachate. Melanin is extracted therefrom by acid precipitation. The secondary leachate is then diluted with water and used to irrigate plants, reducing bacterial diversity and stimulating beneficial microorganisms in the rhizosphere around the plants.

Biocatalytic conversion systems and methods of producing and using same that have improved yields are disclosed. The systems and methods involve co-fermentation of sugars and gaseous substrates for alcohol, ketone, and/or organic acid production. The systems and methods may include biocatalytically converting at least one sugar substrate into at least one of alcohol, at least one ketone, and/or at least one organic acid. The systems and methods may further include biocatalytically converting gases that comprise CO.sub.2 and H.sub.2 to at least one alcohol and/or at least one organic acid, thereby adding extra revenue to biorefineries.

A polyhydroxyalkanoates extraction system comprises a pretreatment subsystem, an extraction subsystem and a recycling subsystem. The pretreatment subsystem comprises a fermentation device and an activation device so as to carry out a microorganism acclimation process. The extraction subsystem comprises a freezing device, a pretreatment device and an extraction device. The extraction subsystem is used for receiving a third sludge so that the third sludge is subjected to a freezing process, a pretreatment process, an extraction process and a purification process in the freezing device to form a polyhydroxyalkanoates mixture, and the extraction device performs a precipitation process to generate polyhydroxyalkanoates precipitate. The recycling subsystem comprises an aerobic sludge digestion device and a sequencing batch reactor activated sludge treatment device so as to carry out an aerobic sludge digestion process and a sequencing batch reactor activated sludge process.

The present disclosure concerns a recombinant bacterial host cell capable of providing a nitrogen source to a yeast during fermentation to make ethanol. The recombinant bacterial host cell is capable of converting a biomass into ethanol. The recombinant bacterial host cell has at least one first genetic modification. The at least one genetic modifications confers to the recombinant bacterial host cell the ability to increase, when compared to a corresponding control bacterial cell lacking the at least one first genetic modification, the proteolytic activity associated with the recombinant bacterial host cell. The at least one genetic modification also confers the recombinant bacterial host cell the ability to provide a nitrogen source to a yeast capable of converting the biomass into ethanol, wherein the nitrogen source comprises a peptide, an amino acid and/or ammonia.

The present disclosure relates to a method that includes utilizing a microorganism for the converting of a substrate to an acid contained in a mixture that includes the acid and water, maintaining a pH of the mixture to less than 5, and treating the mixture with a first stream comprising an organic.

The disclosure relates to the combination of a primary fermentation that converts gas to acetate with a secondary fermentation that converts acetate to a target product. Preferably, the gas contains carbon dioxide, such that the disclosure enables the fixation of carbon dioxide into useful products. The fermentations may be any combination of aerobic and anaerobic, batch and continuous. The fermenting microorganisms may typically be bacterial or fungal.

The disclosure relates to the combination of a primary fermentation that converts gas to acetate with a secondary fermentation that converts acetate to a target product. Preferably, the gas contains carbon dioxide, such that the disclosure enables the fixation of carbon dioxide into useful products. The fermentations may be any combination of aerobic and anaerobic, batch and continuous. The fermenting microorganisms may typically be bacterial or fungal.

The invention relates to a food biopreservative composition comprising a consortium of lactic acid bacteria comprising at least one homofermentative lactic acid bacteria; at least one heterofermentative lactic acid bacteria and an activating agent. The biopreservative composition inhibits the growth of pathogens such as Salmonella, E. coli, Staphylococcus aureus, Listeria monocytogenes, Aspergillus niger and Aspergillus flavus and can prolong the shelf-life of processed and unprocessed food.

The invention relates to a food biopreservative composition comprising a consortium of lactic acid bacteria comprising at least one homofermentative lactic acid bacteria; at least one heterofermentative lactic acid bacteria and an activating agent. The biopreservative composition inhibits the growth of pathogens such as Salmonella, E. coli, Staphylococcus aureus, Listeria monocytogenes, Aspergillus niger and Aspergillus flavus and can prolong the shelf-life of processed and unprocessed food.

Biocatalytic conversion systems and methods of producing and using same that have improved yields are disclosed. The systems and methods involve co-fermentation of sugars and gaseous substrates for alcohol, ketone, and/or organic acid production. The systems and methods may include biocatalytically converting at least one sugar substrate into at least one of alcohol, at least one ketone, and/or at least one organic acid. The systems and methods may further include biocatalytically converting gases that comprise CO.sub.2 and H.sub.2 to at least one alcohol and/or at least one organic acid, thereby adding extra revenue to biorefineries.