Disclosed are compositions and methods for the multiplexed analysis of one or more intracellular targets of a single cell. Exemplary compositions of the disclosure comprise a surface comprising a plurality of capture agents operatively-linked thereto, wherein each capture agent specifically binds to a distinct intracellular target and wherein the plurality of capture agents form a repeating pattern; a substrate comprising a plurality of chambers, wherein the substrate releasably couples with the surface and wherein each chamber of the plurality of chambers comprises at least one repeat of the repeating pattern of the plurality of capture agents of the surface; a coating composition comprising a cell lysis composition; and a linker composition comprising a functionalization component and an extension component.

Provided is an antibacterial agent-containing dried plate having no cracks on an observation surface (a part of the plate corresponding to an observation visual field of a microscope). According to the present embodiment, an antibacterial agent-introduced plate obtained by introducing an antibacterial agent and performing vacuum drying has a recess at an edge of a well, and a microscopic observation portion, which has a surface substantially parallel to a well bottom surface, near the center of the well. The recess is provided between the microscopic observation portion and a side wall of the well, and is lower in height than the microscopic observation portion. Further, at least the bottom surface of the well and the microscopic observation portion are made of a material having a light-transmitting property in order for optical measurement (FIG. 1).

The present invention relates to a novel organic electron-transfer mediator showing an excellent oxidation-reduction potential and a device such as an electrochemical biosensor having improved performance comprising the same.

The present invention relates to a novel organic electron-transfer mediator showing an excellent oxidation-reduction potential and a device such as an electrochemical biosensor having improved performance comprising the same.

A method for detecting an analyte is described in which the simultaneously binding of two fusion proteins (i.e., a sandwich assay in solution) is used, bringing two halves of a split enzyme together to produce product, which is detected via a FRET-based biosensor. The method may incorporate an autocatalytic feedback loop that responds to enzymatic product by producing more product to provide ultrasensitive, bistable detection of analyte that is tunable over several orders of magnitude. This system is broadly applicable for protein and small molecule detection.

A cell culturing system includes an enzyme sensor that detects a concentration of a predetermined component of a culture medium used for culturing of cells, a sensor flow path in which the enzyme sensor is disposed, and a bioreactor that performs culturing of cells while causing the culture medium to flow through the sensor flow path, and further includes a deterioration index calculation unit that determines a deterioration index of the enzyme sensor, based on a detected concentration value of the enzyme sensor, and a detection period during which the detected concentration value is detected, and a lifespan determination unit that detects whether or not an integrated value of the deterioration index has reached a threshold value, and thereby determines the lifespan of the enzyme sensor.

The present invention falls within the field of medical apparatuses. Disclosed are a method and a device for identifying platelet aggregation, and a flow cytometer, which are used for accurately giving an alarm about a platelet aggregation during blood cell analysis. The method comprises: detecting a pre-treated blood sample by using a flow cytometry technique so as to acquire scattered light signals and fluorescent light signals of the blood sample, wherein the scattered light signals are forward scattered light signals or side scattered light signals; differentiating between ghost particles and white blood cells by using a fluorescence-scattered light diagram generated by the scattered light signals and the fluorescent light signals of the blood sample; and counting a number of particles in a ghost characteristic region in the fluorescence-scattered light diagram of the blood sample and determining whether the number of particles exceeds a threshold value, and outputting a warning of platelet aggregation if the number of particles exceeds the threshold value.

This invention relates to a method and device for improving the accuracy and performance of detecting or diagnosing sexually transmitted infections (STIs) or STI-causing pathogens. In one embodiment, the present method and device are related to removing one or more interfering molecules such as urea from urine sample, where these interfering molecules alter the performance of Lateral-Flow Immunoassay (LFA). In one embodiment, an aqueous two-phase system (ATPS) embedded entirely within a porous material allows spontaneous phase separation and the target STI-causing pathogens is concentrated in one of the separated phases. In one embodiment, a detection module such as the Lateral-Flow Immunoassay (LFA) is used in connection with other modules so as to detect or diagnose the sexually transmitted infections or the pathogens associated with STIs with an improved performance.

A method for quantifying a target substance, comprising: bringing a sample containing the target substance into contact with a biosensor which comprises an enzyme electrode containing an oxidoreductase and a counter electrode; measuring a change in the potential difference between the enzyme electrode and the counter electrode due to oxidation reaction of the target substance catalyzed by the oxidoreductase; and calculating the concentration of the target substance based on the change in the potential difference; wherein a potential is applied between the enzyme electrode and the counter electrode before the measurement of the change in the potential difference.

A method for quantifying a target substance, comprising: bringing a sample containing the target substance into contact with a biosensor which comprises an enzyme electrode containing an oxidoreductase and a counter electrode; measuring a change in the potential difference between the enzyme electrode and the counter electrode due to oxidation reaction of the target substance catalyzed by the oxidoreductase; and calculating the concentration of the target substance based on the change in the potential difference; wherein a potential is applied between the enzyme electrode and the counter electrode before the measurement of the change in the potential difference.