Disclosed are methods, systems, components, and compositions for cell-free synthesis of glycosylated proteins. The glycosylated proteins may be utilized in vaccines, including anti-bacterial vaccines. The glycosylated proteins may include a bacterial polysaccharide conjugated to a carrier, which may be utilized to generate an immune response in an immunized host against the polysaccharide conjugated to the carrier. The glycosylated proteins may be synthesized in cell-free glycoprotein synthesis (CFGpS) systems using prokaryote cell lysates that are enriched in components for glycoprotein synthesis such as oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs) including OSTs and LLOs associated with synthesis of bacterial O antigens.

The present invention relates to a Bifidobacterium breve JKL2022 strain capable of mass-producing natural conjugated linoleic acid (CLA), and a method for mass-producing conjugated linoleic acid using the same. The method of the present invention makes it possible to easily produce natural CLA in large quantities and high purity of functional CLA in a linoleic acid solution (50 mg/ml) using bacterial cells or cell-free extracts. The cell or cell-free extract of Bifidobacterium breve JKL2022 strain of the present invention, and the conjugated linoleic acid (CLA) produced thereby can be widely used in probiotic compositions, pharmaceutical compositions, health functional food compositions, feed additive compositions, and food additive compositions.

Food products are provided in which microbial protein sources, such as single cell protein or protein hydrolysis products, are incorporated, and which contain proteins that are recombinantly expressed in chemoautotrophic microorganisms. Meat, dairy, and egg substitute products are provided that include microbial protein and proteins that are recombinantly produced in chemoautotrophic microorganisms in place of animal protein.

The present disclosure pertains to a Methanothermobacter wolfeii archaeal strain identified as BSEL1 and progeny and mutants thereof. The archaeal strain BSEL1 is capable of growing and producing renewable natural gas (RNG) from a supply of carbon dioxide (CO.sub.2) and hydrogen (H.sub.2) without the addition of organic supplements, and further to grow in wastewater with an exceptionally high growth rate. The strain is further capable of reducing CO.sub.2 to a very low level when grown, e.g., in a trickling bed reactor. BSEL1 is of major importance for direct addition of RNG into the natural gas grid.

A method for producing a microbial oil includes genetically modifying a labyrinthulid which belongs to the genus Parietichytrium or the genus Schizochytrium by disrupting and/or silencing a 4 desaturase gene, a C20 elongase gene, and/or a C18 elongase gene, or by being transformed with an 3 desaturase gene; culturing the labyrinthulid; and collecting the microbial oil from the labyrinthulid. The labyrinthulid has an activity of synthesizing DHA via a PUFA-PKS pathway in an amount of not more than 1/100 of a total amount of DHA synthesized in the labyrinthulid or no activity of producing PUFAs via the PUFA-PKS pathway, and the labyrinthulid has an activity of producing PUFAs via an endogenous elongase-desaturase pathway.

The present invention provides Paraburkholderia sp. LSOJM18 and use thereof as well as a microbial inoculum, belonging to the technical field of microorganisms. The Paraburkholderia sp. LSOJM18 was deposited to the China Center for Type Culture Collection on Dec. 29, 2022, with the depository number CCTCCNO: M20222102. With a strong phosphate solubilization capacity, the strain provided by the present invention is suitable for activating phosphorus in farmland soil. Using the phosphate-solubilizing bacteria can significantly increase the available phosphorus content of soil and reduce the application of chemical phosphate fertilizers. Acting on a wide range of substrates, the phosphate-solubilizing bacteria strain provided by the present invention can not only secrete phosphatase to hydrolyze organic phosphorus, but also release small molecules such as organic acids, etc., to activate the fixed inorganic phosphorus, and using the phosphate-solubilizing bacteria can significantly improve the phosphorus activation capacity of soil.