This invention provides compositions and methods for providing high level expression of proteins of interest in cyanobacteria.

The present invention relates to a mutant organism having the ability to produce aspartic acid derivatives, wherein a gene encoding the glyoxylate shunt regulator and a gene encoding fumarase are deleted and a gene encoding aspartase is overexpressed compared to that in a wild-type strain, and to a method for producing L-aspartic acid derivatives using the same. According to the present invention, various aspartic acid derivatives, including L-alanine, 3-aminopropionic acid, threonine, 1,3-diaminopropane, lysine, methionine, 3-hydroxypropionic acid, cadaverine, 5-aminovaleric acid, etc., can be produced by biological methods.

Disclosed are methods, systems, components, and compositions for cell-free synthesis of glycosylated proteins. The glycosylated proteins may be utilized in vaccines, including anti-bacterial vaccines. The glycosylated proteins may include a bacterial polysaccharide conjugated to a carrier, which may be utilized to generate an immune response in an immunized host against the polysaccharide conjugated to the carrier. The glycosylated proteins may be synthesized in cell-free glycoprotein synthesis (CFGpS) systems using prokaryote cell lysates that are enriched in components for glycoprotein synthesis such as oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs) including OSTs and LLOs associated with synthesis of bacterial O antigens.

The present invention provides Paraburkholderia sp. LSOJM18 and use thereof as well as a microbial inoculum, belonging to the technical field of microorganisms. The Paraburkholderia sp. LSOJM18 was deposited to the China Center for Type Culture Collection on Dec. 29, 2022, with the depository number CCTCCNO: M20222102. With a strong phosphate solubilization capacity, the strain provided by the present invention is suitable for activating phosphorus in farmland soil. Using the phosphate-solubilizing bacteria can significantly increase the available phosphorus content of soil and reduce the application of chemical phosphate fertilizers. Acting on a wide range of substrates, the phosphate-solubilizing bacteria strain provided by the present invention can not only secrete phosphatase to hydrolyze organic phosphorus, but also release small molecules such as organic acids, etc., to activate the fixed inorganic phosphorus, and using the phosphate-solubilizing bacteria can significantly improve the phosphorus activation capacity of soil.

A method for producing a shaped element made of bacterial nanocellulose includes providing a shaped article, providing a growth medium for bacterial nanocellulose comprising Acetobacteraceae bacteria of the genus Komagataeibacter and the species Komagataeibacter hansenii or K. hansenii, preferably in the form of a bacterial suspension, and a nutrient solution for the bacteria, bringing a part of the shaped article into contact with the growth medium for bacterial nanocellulose, and rotating the shaped article to obtain the shaped element made of bacterial nanocellulose. Preferably, rotating the shaped article is carried out at a rotational speed of higher than 10 rpm and maximum 60 rpm and/or wherein the ratio of the bacterial suspension to the nutrient solution is 1:18.

The present invention discloses a microbially induced carbonate precipitation (MICP)-based vertical barrier, and a preparation method thereof, wherein a raw material of the vertical barrier includes in-situ soil, Ca-bentonite, a bacteria-containing culture medium, and a cementing solution. The bacteria-containing culture medium comprises flocculent ureolytic bacteria flora. The proportions of dry the Ca-bentonite to in-situ soil, the bacterial-containing culture medium to the cementing solution, and the cementing solution to the Ca-bentonite are specified to be 1:2, (25):1, and (12.5):10, respectively, to ensure optimal performance. The present invention addresses challenges related to the insufficient impermeability of Ca-bentonite-based barriers in the application of vertical barrier, and aims to reduce both the cost and carbon emissions associated with their construction.

Disclosed are compositions and methods related to eukaryotic microorganisms that can produce unsaturated fatty acids which can be purified and used.

New Lactococcus lactis strains, NRRL B-50571 and NRRL B-50572, and a bacterial preparation containing the same, have the ability to produce bioactive peptides that reduce blood pressure, lower LDL-cholesterol (bad cholesterol) and present antioxidant properties for better cardiovascular health. These biologically active peptides may be produced within the food for the production of a food product, such as a functional food, or they may be produced from protein sources and subsequently added to a food as part of the formulation or as part of a food supplement or a pharmaceutical preparation.

New Lactococcus lactis strains, NRRL B-50571 and NRRL B-50572, and a bacterial preparation containing the same, have the ability to produce bioactive peptides that reduce blood pressure, lower LDL-cholesterol (bad cholesterol) and present antioxidant properties for better cardiovascular health. These biologically active peptides may be produced within the food for the production of a food product, such as a functional food, or they may be produced from protein sources and subsequently added to a food as part of the formulation or as part of a food supplement or a pharmaceutical preparation.

The present invention provides a method of the proliferation of pest infestation using a species of Burkholderia. Also provided are natural products derived from a culture of said species and methods of controlling algae and/or arachnids using said natural products.