The present invention provides methods and apparatus for measuring a property of an sample, the apparatus can manipulate, detect, and analyze the sample composed of single molecules, single small particles or single small samples of matter by drawing the sample into a nanofluidic channel and stretching the sample within the channel, passing the stretched sample through a gap having a width of less than or equal to 20 nm of a nanogap detector positioned inside or adjacent to the nanofluidic channel and measuring an output from the nanogap detector representative of the property of the sample.

The invention includes compositions, devices, and methods for analyzing a polymer and/or polymer unit. The polymer may be a homo or hetero-polymer such as DNA, RNA, a polysaccharide, or a peptide. The device includes electrodes that form a tunnel gap through which the polymer can pass. The electrodes are functionalized with a reagent attached thereto, and the reagent is capable of forming a transient bond to a polymer unit. When the transient bond forms between the reagent and the unit, a detectable signal is generated and used to analyze the polymer.

The use of ion sensitive field effect transistor (ISFET) to detect methylated nucleotides in a DNA sample is described. A method of detecting methylated nucleotides in a DNA sample may include the steps of treating a sample of DNA with a reagent which discriminates between methylated and non-methylated nucleotides to provide treated DNA, amplifying the treated DNA and optionally sequencing the amplified DNA. An ISFET is used to monitor the addition of one or more dNTPs in the strand extension reactions during the amplification and/or sequencing step. Suitable apparatus is also provided.

An electrode system configured to be positioned within a vacuum chamber of an electron-beam metal evaporation and deposition apparatus including a metal slug from which metal is evaporated during operation of the electron-beam metal evaporation and deposition apparatus. The electrode system includes a substantially ring-shaped electrode formed of a conductive material and a plurality of insulating standoffs configured to support the substantially ring-shaped electrode in the vacuum chamber in a position substantially surrounding the metal slug.

An electrode system configured to be positioned within a vacuum chamber of an electron-beam metal evaporation and deposition apparatus including a metal slug from which metal is evaporated during operation of the electron-beam metal evaporation and deposition apparatus. The electrode system includes a substantially ring-shaped electrode formed of a conductive material and a plurality of insulating standoffs configured to support the substantially ring-shaped electrode in the vacuum chamber in a position substantially surrounding the metal slug.

A glazing having a device for detecting a crack in the glazing comprises a sensor, substantially circumscribing a ply of glazing material. A surface contact, bonded to the glazing, is configured with a portion of the sensor to form a capacitive coupling for transferring AC signals in a frequency range. Preferably the sensor is frangible and voltage may be applied by a second surface contact. Alternatively, the glazing further comprises an electrically resistive element, such as a coating for heating the glazing, and voltage may be applied to the sensor by a spur from a busbar such that damage to coating, busbar or sensor resulting in arcing is detectable at the surface contact. An electronics module attached to the surface contact generates an alarm.

A glazing having a device for detecting a crack in the glazing comprises a sensor, substantially circumscribing a ply of glazing material. A surface contact, bonded to the glazing, is configured with a portion of the sensor to form a capacitive coupling for transferring AC signals in a frequency range. Preferably the sensor is frangible and voltage may be applied by a second surface contact. Alternatively, the glazing further comprises an electrically resistive element, such as a coating for heating the glazing, and voltage may be applied to the sensor by a spur from a busbar such that damage to coating, busbar or sensor resulting in arcing is detectable at the surface contact. An electronics module attached to the surface contact generates an alarm.

Described herein are cell culture interfaces that can be configured to relay an energy between a cell and an electronic interface and methods of using the cell culture interfaces that can be configured to relay an energy between a cell and an electronic interface.

The disclosed subject matter provides a nanoaperture having a bottom surface and a side wall comprising gold. A surface of the side wall is passivated with a first functional molecule comprising polyethylene glycol. The bottom surface of the nanoaperture can be functionalized with at least one second molecule comprising polyethylene glycol, for example, a silane-PEG molecule. The second molecule can further include a moiety, such as biotin, which is capable of binding a target biomolecule, which in turn can bind to a biomolecule of interest for single molecule fluorescence imaging analysis. Fabrication techniques of the nanoaperture are also provided.

One feature pertains to a microdosimeter cell array that includes a plurality of microdosimeter cells each having a semiconductor volume adapted to generate a current in response to incident radiation. The semiconductor volumes of each of the plurality of microdosimeter cells have at least one of a size, a shape, a semiconductor type, and/or a semiconductor doping type and concentration that is associated with one or more cells or cell components of a human eye. A processing circuit is also communicatively coupled to the microdosimeter cell array and generates a signal based on the currents generated by the semiconductor volumes of the plurality of microdosimeter cells. The signal generated by the processing circuit is indicative of an amount of radiation absorbed by the microdosimeter cell array.