A method for highly-sensitive and rapid detection of a pesticide residue based on an imprinted metal-organic framework (MOF) probe is provided. A molecularly imprinted MOF enzyme-mimic probe is used as a colorimetric probe to catalyze the oxidation of a substrate, thereby enabling a color change of a system; a low-cost filter paper is used as a substrate for supporting the colorimetric probe, including a quality control zone, a standard zone, and a detection zone; in the quality control zone, the optimal colorimetric analysis parameters can be selected according to the temperature, humidity, and light, etc. of an environment to be tested; the standard zone is a standard colorimetric zone obtained through the dropwise addition of standards with different concentrations and is provided to establish a colorimetric analysis mathematical model; and the detection zone is provided for the detection of an actual sample.

A polymerization process, preferably a living radical polymerisation process, is carried out in the presence of a template substance, to produce molecularly imprinted polymers (MIPs). It is controlled so that the products are quite small (500-10.sup.6 Daltons) so that they are soluble or form colloidal suspensions. The template substances are immobilized templates, which can be re-used. Immobilized templates are also useful for purification of MIP solutions/suspensions by affinity chromatography.

In a first aspect, the present disclosure relates to a method for immobilizing a molecularly imprinted polymer onto a substrate, comprising providing a substrate having an amorphous carbon surface; and grafting the molecularly imprinted polymer onto the amorphous carbon surface.

Methods and devices for the detection of food allergens using molecularly imprinted polymers that are imprinted for a target food allergen. A molecularly imprinted polymer may be imprinted using surface imprinting or other procedures. Detection of food allergens, such as peanut allergens, may be accomplished using all or a portion of a protein food allergen as a template to produce a molecularly imprinted polymer for food allergen detection. A portion utilized can be one that creates receptor sites in the molecularly imprinted polymer that are unique or more unique to the target food allergen than receptor sites that would be created if an entire food allergen molecule were utilized.

The present disclosure provides a method for detecting a biomolecule. The method includes providing an electrode 101 and an aptamer 102 that specifically binds to a target biomolecule and has a charge, the aptamer 102 being disposed near the electrode 101; introducing a cationic mediator 113 having a charge opposite to the charge of the aptamer 102 to the electrode 101 at which the aptamer 102 is disposed; bringing a solution containing the target biomolecule into contact with the aptamer 102 to cause the aptamer 102 to bind to the biomolecule; and measuring an electrical signal that is produced at the electrode 101 in association with the cationic mediator 113.

Molecularly imprinted particles comprising a label are described herein, in particular such particles prepared by RAFT polymerization, as are compositions and kits comprising such particles, and methods for preparing and using such particles.

A reusable test device includes: a wick adapted to collect a fluid to be analyzed; a reusable sensor unit, including: a layer (8) having at least one type of molecular imprinted polymer adapted to bind at least one analyte present in said fluid; and a layer (7) having at least one electrode, wherein said reusable sensor unit is regenerable upon cleansing. The reusable test device further includes a rechargeable electronic unit adapted to read out results from said reusable sensor unit.

This invention provides molecularly imprinted polymer nanoparticles compatible with biological samples, and in particular pure biological samples and a preparation method thereof. Said molecularly imprinted polymer nanoparticles have a crosslinking degree exceeding 50%, a particle diameter of 10 to 500 nm, hydrophilic polymer brushes on its surfaces and can be prepared by introducing appropriate hydrophilic macromolecular chain transfer agents into reversible addition-fragmentation chain transfer (RAFT) precipitation polymerization systems through the one-pot synthesis. The preparation method is simple, features a broad range of application and yields a pure product. The obtained hydrophilic molecularly imprinted polymer nanoparticles have prospects for a wide range of application in biological sample analysis, medical clinical immune analysis, food and environmental monitoring, biomimetic sensors, etc.

Provided is a chemical analysis apparatus configured to quickly quantify and detect target molecules at low costs with high-sensitivity. A chemical analysis apparatus 600 includes a pretreating unit 60 in which a capturing body 61 that captures a target molecule using a molecularly imprinted polymer interacting with the target molecule included in a specimen is accommodated, a specimen introducing unit 62 that supplies the specimen to the pretreating unit 60, a desorbing liquid supplying unit 67 that supplies a desorbing liquid, which desorbs the target molecule from the capturing body 61, to the pretreating unit 60, and a quantifying unit 65 that quantifies the target molecules desorbed from the capturing body 61 using the desorbing liquid.

The present disclosure relates to a method for characterizing and identifying a bioparticle. The method comprises introducing the sample to a substrate having a surface comprising a plurality of binding sites whereon bioparticles can be bound, determining, for at least one temperature, data representative for the interface thermal resistance of the surface of the substrate sufficiently long to include the detachment process of the bioparticles, and deriving, for the at least one temperature, a bioparticle retention time and/or detachment rate from the data representative for the interface thermal resistance data. The present disclosure also relates to a bio-sensing device suitable for the detection and/or characterization of target bioparticles.