A system and method for testing of active molecules using simulation of skin membrane have been provided. The present disclosure provides a molecular level model of the skins upper protective layer Stratum-Corneum. The systems consist of a molecular model of the skins upper layer stratum corneum and permeate molecules. A protocol have been developed to perform molecular dynamics simulations which can be automated. The system predicts the permeability, partition coefficient and diffusivity of different active molecules like drugs and cosmetics through mentioned skin model using multiple molecule in single window constrained molecular dynamics simulations.

Methods for treating cancer using compounds that inhibit human DNA ligases. Methods for using compounds that inhibit human DNA ligases to provide insights into the reaction mechanisms of human DNA ligases, for example to identify the human DNA ligase involved in different DNA repair pathways. Screening methods for compounds that inhibit human DNA ligases.

Techniques for identifying production-fit amino acid sequence libraries are disclosed. The techniques may include accessing a statistical model relating an input amino acid sequence to production fitness of a protein having the input amino acid sequence, obtaining production fitness information for production-fit variant amino acid sequences, and generating an amino acid sequence library having amino acid sequences with predicted production fitness in accordance with the production fitness information. The techniques further include using a statistical model for a protein characteristic other than production fitness to generate an amino acid sequence library having amino acid sequences that are both predicted to be production-fit and have the protein characteristic.

The present invention relates to a method for producing a population of nucleic acids encoding at least one protein comprising at least one immunoglobulin variable domain having a non-human-derived CDR3 amino acid sequence embedded in essentially human framework sequences, as well as to a population of nucleic acids and a population of proteins relates thereto and uses thereof.

The present invention relates to a method for producing a population of nucleic acids encoding at least one protein comprising at least one immunoglobulin variable domain having a non-human-derived CDR3 amino acid sequence embedded in essentially human framework sequences, as well as to a population of nucleic acids and a population of proteins relates thereto and uses thereof.

Provided herein are apparatus for independently manipulating the temperature of a plurality of reaction vessels, e.g., for automated processing of nucleic acids present in the vessels. Printed circuit boards (PCBs) comprising a mount area arranged to have mounted thereon a through-hole thermoelectric device (TED) to facilitate independent temperature control of reaction vessels are also provided, as well as methods relating to the same.

A computational method of modeling a bioreactor combines mechanistic models of kinetics of metabolic fluxes and flux balance analysis to predict cell culture performance. The mechanistic models include effects of process variables descriptive of extracellular environment, e.g., temperature, acidity, osmolarity, and/or metabolite concentrations. The method constrains flux rates based on the mechanistic models and computes the flux rates in view of suitable metabolic objectives. The method simulates time evolution of process variables of the bioreactor based on user input and computes performance metrics to display to the user, to control a bioreactor, and/or to train an artificial intelligence model of a bioreactor.

A computational method of modeling a bioreactor combines mechanistic models of kinetics of metabolic fluxes and flux balance analysis to predict cell culture performance. The mechanistic models include effects of process variables descriptive of extracellular environment, e.g., temperature, acidity, osmolarity, and/or metabolite concentrations. The method constrains flux rates based on the mechanistic models and computes the flux rates in view of suitable metabolic objectives. The method simulates time evolution of process variables of the bioreactor based on user input and computes performance metrics to display to the user, to control a bioreactor, and/or to train an artificial intelligence model of a bioreactor.

This disclosure provides systems and methods for sample processing and data analysis. Sample processing may include nucleic acid sample processing and subsequent sequencing. Some or all of a nucleic acid sample may be sequenced to provide sequence information, which may be stored or otherwise maintained in an electronic storage location. The sequence information may be analyzed with the aid of a computer processor, and the analyzed sequence information may be stored in an electronic storage location that may include a pool or collection of sequence information and analyzed sequence information generated from the nucleic acid sample. Methods and systems of the present disclosure can be used, for example, for the analysis of a nucleic acid sample, for producing one or more libraries, and for producing biomedical reports. Methods and systems of the disclosure can aid in the diagnosis, monitoring, treatment, and prevention of one or more diseases and conditions.

This disclosure provides systems and methods for sample processing and data analysis. Sample processing may include nucleic acid sample processing and subsequent sequencing. Some or all of a nucleic acid sample may be sequenced to provide sequence information, which may be stored or otherwise maintained in an electronic storage location. The sequence information may be analyzed with the aid of a computer processor, and the analyzed sequence information may be stored in an electronic storage location that may include a pool or collection of sequence information and analyzed sequence information generated from the nucleic acid sample. Methods and systems of the present disclosure can be used, for example, for the analysis of a nucleic acid sample, for producing one or more libraries, and for producing biomedical reports. Methods and systems of the disclosure can aid in the diagnosis, monitoring, treatment, and prevention of one or more diseases and conditions.