The present invention provides high-throughput methods capable of screening compounds for herbicidal activity or plant growth regulating activity and further allows for the prediction of the mode of action of herbicidal compounds or plant growth regulators. The methods provided herein also facilitate the identification of mutation/s responsible for resistance to herbicides in plants and the identification of the herbicide target. The methods further provide for the identification of mutation/s responsible for plant growth regulation and the identification of the plant growth regulator target.

The present invention relates to a method of providing a seedstock of Sphagnum. The method comprises providing in vitro Sphagnum, applying the Sphagnum to a growth surface, and cultivating the Sphagnum in vivo on the growth surface. The method also comprises harvesting the cultivated Sphagnum from the growth surface, and then chopping the harvested Sphagnum to provide a seedstock of Sphagnum for cultivation, the seedstock comprising a plurality of fragments of the in vivo Sphagnum. The present invention also relates to Sphagnum and seedstocks of Sphagnum obtainable by the method.

The present invention relates to a method of providing a seedstock of Sphagnum. The method comprises providing in vitro Sphagnum, applying the Sphagnum to a growth surface, and cultivating the Sphagnum in vivo on the growth surface. The method also comprises harvesting the cultivated Sphagnum from the growth surface, and then chopping the harvested Sphagnum to provide a seedstock of Sphagnum for cultivation, the seedstock comprising a plurality of fragments of the in vivo Sphagnum. The present invention also relates to Sphagnum and seedstocks of Sphagnum obtainable by the method.

An isolated polynucleotide encoding a fusion protein which comprises a DNA targeting moiety linked to a catalytic domain of a plant DNA methyltransferase 3 (DNMT3) protein is disclosed. Uses thereof are also disclosed.

An isolated polynucleotide encoding a fusion protein which comprises a DNA targeting moiety linked to a catalytic domain of a plant DNA methyltransferase 3 (DNMT3) protein is disclosed. Uses thereof are also disclosed.

An apparatus (100) for use in culturing Sphagnum (106) is provided. The apparatus (100) comprises a culture vessel (102) for Sphagnum (106) and a culture medium (104) arranged in the culture vessel (102). The culture vessel (102) comprises an enclosed airspace (108) above the culture medium (104), and Sphagnum (106) arranged in the culture medium (104). The apparatus (100) further comprises means (110, 112) for supplying carbon dioxide into the enclosed airspace (108).

An apparatus (100) for use in culturing Sphagnum (106) is provided. The apparatus (100) comprises a culture vessel (102) for Sphagnum (106) and a culture medium (104) arranged in the culture vessel (102). The culture vessel (102) comprises an enclosed airspace (108) above the culture medium (104), and Sphagnum (106) arranged in the culture medium (104). The apparatus (100) further comprises means (110, 112) for supplying carbon dioxide into the enclosed airspace (108).

A method for in vitro culturing Sphagnum is provided. The method comprises providing a vegetative fragment of Sphagnum and surface cleaning the fragment with alcohol. The method further comprises dissecting the fragment to provide a piece (106) of Sphagnum. The method further comprises initiating the piece (106) of Sphagnum in a culture medium (104).

A method for in vitro culturing Sphagnum is provided. The method comprises providing a vegetative fragment of Sphagnum and surface cleaning the fragment with alcohol. The method further comprises dissecting the fragment to provide a piece (106) of Sphagnum. The method further comprises initiating the piece (106) of Sphagnum in a culture medium (104).

The present application relates to plant growth promoting microbes (PGPMs), compositions comprising these PGPMs and methods of using these PGPMs and/or compositions for enhancing plant health, plant growth and/or plant yield, and/or for preventing, inhibiting, or treating the development of plant pathogens or the development of phytopathogenic diseases. This application also provides non-naturally occurring plant varieties that are artificially infected with a PGPM descried herein, as well as seed, reproductive tissue, vegetative tissue, regenerative tissues, plant parts, or progeny thereof.