The present invention relates to a new in vitro method for direct regeneration of a Cannabis sativa L. plant as well as the use of such method for micropropagation of selected elite clones belonging to Cannabis sativa L., development of polyploid Cannabis plants with enhanced levels of secondary metabolites, promotion of spontaneous rooting of in vitro regenerants in a shorter period of time than conventionally used methods, and production of mutagenized and transgenic or gene-edited Cannabis plants. The method advantageously comprises culturing selected explants lacking already developed meristems such as cotyledons, hypocotyls and/or epicotyls from Cannabis seedlings of short and neutral-day varieties in an appropriate culture medium without plant growth regulators nor chemical microtubule disruptors with a high toxicity grade.

Embodiments described herein provide for multi-media structures 100 with growth enhancement additives for multiple stages of growth of an organism such as a plant, fungus or bacteria, including the production of individual media structures and multi-media structures 100 for multi-stage growth. Methods for the production of individual media structure and multi-media structures 100 with growth enhancement additives are provided. Methods for using multi-media structures 100 to grow an organism through multiple stages of growth such as root production, vegetative growth and flowering are also provided.

The problem to be solved by this invention is to provide a method of gene introduction for a genus Sorghum plant and a method for producing a transformed genus Sorghum plant with a higher efficiency than the conventionally known Agrobacterium method. This invention provides a gene introduction method of a genus Sorghum plant characterized by using a medium with an increased concentration of a nitrogen source and/or an increased concentration of inorganic ions selected from magnesium ion, potassium ion, calcium ion and sodium ion, in a step of preparing a plant tissue material of a genus Sorghum plant, a step of inoculation with Agrobacterium and/or a co-cultivation step. This invention also provides a method for producing a transformed plant using the gene introduction method of this invention.

The invention provides seed and plants of pepper hybrid SV5603PB and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of pepper hybrid SV5603PB and the parent lines thereof, and to methods for producing a pepper plant produced by crossing such plants with themselves or with another pepper plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

The present invention relates to the use of mammal kinase inhibitors, preferably human kinase inhibitors, to promote the induction of in vitro embryogenesis, a strategy never used in plants systems before. The results obtained indicated that these inhibitors have beneficial effects in both crop and forest plants in in vitro systems of microspore and somatic embryogenesis.

The present invention relates to the field of plant breeding and in particular to the regeneration of plants from cells and other tissues. More particularly, the invention provides methods and means for improving callus formation and regeneration of plants from callus tissue using a histone deacetylase inhibitor.

The present invention relates to the use of trehalose, and/or a derivative thereof, as a supplement to a culture medium for culturing hybrid plant embryos in in vitro embryo rescue. The addition of trehalose, and/or a derivative thereof, to the culture medium significantly increased the survival rate of the hybrid plant embryos.

A method for organically planting Dendrobium, including using a planting medium in the epiphytic planting step of Dendrobium. The raw materials of planting medium include fermented powder of Dendrobium candidum leaves and sawdust; a method for preparing the fermented powder of Dendrobium candidum leaves is as follows: cutting Dendrobium candidum leaves into small pieces and mashing the small pieces of Dendrobium candidum leaves to obtain materials to be fermented, and then adding liquid medium to the materials to be fermented to obtain fermentation system; sterilizing the fermentation system, adding fermentation bacteria to the fermentation system to obtain a mixture, fermenting and filtering the mixture to obtain post-culturing fermentation broth and fermented products of Dendrobium candidum leaves, dewatering and crushing the fermented products of Dendrobium candidum leaves to obtain fermented powder of Dendrobium candidum leaves.

A method of producing cannabinoids for use in medical treatments by growing cultured Cannabis sativa plant cells through tissue culture, the method of comprising the steps of: selecting Cannabis sativa leaf tissue for culture; and growing a tissue culture from the selected leaf tissue in a liquid based medium whilst controlling the light exposure of the tissue culture to control the cannabinoid content of the tissue culture. Control of the light exposure can enable the phytocannabinoid content of the grown tissue culture to be tailored to the use intended for the tissue culture. For example, the THC content of the tissue culture can be controlled to be maximised or minimized depending on the intended use. Use of tissue culture is beneficial as compared to prior art methods as it allows for genetic consistency and reduces the resources necessary to produce plant cells containing phytocannabinoids.

New lettuce variety designated ‘E01G.11092’ is described. ‘E01G.11092’ is a lettuce variety exhibiting stability and uniformity.