The present invention relates to an isolated transgenic pig beta cell wherein the PKC and the PKA pathway are constitutively activated; to a transgenic pig islet comprising said transgenic pig beta cell; and to a transgenic pig comprising said transgenic pig beta cell or said transgenic pig islet. Another object of the invention is a device comprising a transgenic pig beta cell or a transgenic pig islet of the invention. The present invention also relates to the use of said transgenic pig beta cell, said transgenic pig islet, or said device for treating a disease, disorder or condition related to the impaired function of endocrine pancreas or of beta cell.

A method is provided of producing a Lactuca plant seed including using a Megachile bee as pollination means, and providing the a Megachile bee a minor nectar plant to have preference in flower visiting, in addition to a pollen parent Lactuca plant and a seed parent Lactuca plant and to perform a crossing of a Lactuca plant using a plant species having the preference in flower visiting equivalent to or lower than that of the Lactuca plant. According to one embodiment, it is possible to produce a Lactuca hybrid plant seed more efficiently and economically than before while using a Megachile bee as pollination means.

The present disclosure is directed to transgenic animals (e.g., transgenic porcine animals) comprising multiple genetic modifications that advantageously render these animals suitable donors for xenotransplanation. The present disclosure extends to organs, organ fragments, tissues and cells derived from these animals and their therapeutic use. The present disclosure further extends to methods of making such animals. In certain embodiments, the transgenic animals (e.g., transgenic porcine animals) have reduced expression of the growth hormone receptor (GHR) gene or have impaired function of the GHR protein.

The disclosure in the specification relates to an artificial recombinant chromosome and the use thereof, and more particularly to an artificial recombinant chromosome generated by the recombination of two or more chromosomes and a production of a transgenic animal using a cell including the same. Especially, in the disclosure in the specification, an interchromosomal exchange between the recipient chromosome and the donor chromosome has many merits to produce the artificial recombinant chromosome for producing the transgenic animal.

In a housing (1), an internal space capable of housing an insect is formed, an opening (11) through which the internal space and an outside communicate with each other is formed, and a portion other than the opening (11) is closed so that the insect is incapable of passing through the portion. Rails (2) in a pair are disposed in parallel with each other on both sides of the opening (11) across the opening (11) on an outer surface of the housing (1), maintain a gap between a face, on which the opening (11) is formed, of the housing (1) and a cover (3) to have a size preventing the insect from passing through the gap, and slidably retain the cover (3) on the external side of the housing (1). A sample holder retains a sample, is slidably retained by the rails (2), and enables the sample to be exposed from the opening (11) to the internal space when being slidably retained by the rails (2) in the state of retaining the sample. The cover (3) and the sample holder retaining the sample can be slid between the rails (2) in the pair in a state in which one side of the cover (3) and one side of the sample holder come into contact with each other, whereby the cover (3) and the sample holder can be moved between a position at which the cover (3) covers the opening (11) and a position at which the sample covers the opening (11).

The disclosure relates to improved methods of cyto-plasmic incompatibility-based transgenics for pest or vector control. Further disclosed are improved gene drivers for use in genetically modified arthropods and use in methods for controlling and/or reducing arthropod populations.

The disclosure in the specification relates to an artificial recombinant chromosome and the use thereof, and more particularly to an artificial recombinant chromosome generated by the recombination of two or more chromosomes and a production of a transgenic animal using a cell including the same. Especially, in the disclosure in the specification, an interchromosomal exchange between the recipient chromosome and the donor chromosome has many merits to produce the artificial recombinant chromosome for producing the transgenic animal.

A method is provided of producing a Lactuca plant seed including using a Megachile bee as pollination means, and providing the a Megachile bee a minor nectar plant to have preference in flower visiting, in addition to a pollen parent Lactuca plant and a seed parent Lactuca plant and to perform a crossing of a Lactuca plant using a plant species having the preference in flower visiting equivalent to or lower than that of the Lactuca plant. According to one embodiment, it is possible to produce a Lactuca hybrid plant seed more efficiently and economically than before while using a Megachile bee as pollination means.

In a housing (1), an internal space capable of housing an insect is formed, an opening (11) through which the internal space and an outside communicate with each other is formed, and a portion other than the opening (11) is closed so that the insect is incapable of passing through the portion. Rails (2) in a pair are disposed in parallel with each other on both sides of the opening (11) across the opening (11) on an outer surface of the housing (1), maintain a gap between a face, on which the opening (11) is formed, of the housing (1) and a cover (3) to have a size preventing the insect from passing through the gap, and slidably retain the cover (3) on the external side of the housing (1). A sample holder retains a sample, is slidably retained by the rails (2), and enables the sample to be exposed from the opening (11) to the internal space when being slidably retained by the rails (2) in the state of retaining the sample. The cover (3) and the sample holder retaining the sample can be slid between the rails (2) in the pair in a state in which one side of the cover (3) and one side of the sample holder come into contact with each other, whereby the cover (3) and the sample holder can be moved between a position at which the cover (3) covers the opening (11) and a position at which the sample covers the opening (11).

A method and system for breeding insects, using a plurality of individual crates, wherein at least a portion of each crate is filled with a substrate, containing feed stock, and immature phases of insects. Also provided is a climate area housing the crates that has an aeration system. A conveyor system is included in the system for retrieving crates from the climate area and for returning same thereto. An observation system for obtaining observations, including data and measurements, and downstream thereof a feedstock supply station are arranged along the conveyor system. The method includes steps of aerating the substrate and the immature phases of insects with the aeration system, when in the climate area; periodically retrieving at least one individual crate of the plurality of crates by the conveyor system from the climate area and passing it to the observation system; obtaining an observation of the substrate and the immature phases of insects in the at least one retrieved crate; and interpreting the observation of a retrieved individual crate and determining one of a requirement for adding an amount of supplementary feed stock, and a reason to withdraw the insects from further breeding for harvesting or discarding from further breeding. The method and system are further arranged for returning the retrieved individual crate to the climate area via the feedstock supply station, when a required amount of supplementary feedstock has been determined, and transferring the retrieved individual crate to one of an area for harvesting and an area for discarding, when a reason for withdrawal has been determined. The steps of the method are repeated for as long as at least one of the plurality of crates remains in the climate area.