A smokeless tobacco product is provided that includes a tobacco material in the form of a tobacco extract, a particulate tobacco material, or a combination thereof. In some embodiments, the smokeless tobacco product is substantially translucent. Methods for making and using the smokeless tobacco product are also provided. The smokeless tobacco product can include isomalt, maltitol syrup, particulate tobacco, and a translucent tobacco extract prepared by ultrafiltration.

Provided are (i) a tobacco plant which is suitable for cultivation for harvesting leaf tobaccos, (ii) a method of obtaining the tobacco plant, (iii) a harvest from the tobacco plant, and (iv) a processed product of the harvest. The present invention encompasses (i) a tobacco plant into which a mutation for suppressing the development of primary axillary buds is introduced, (ii) a method of obtaining the tobacco plant, (iii) a harvest from the tobacco plant, and (iv) a processed product of the harvest.

Provided is a tobacco plant which is suitable for cultivation for harvesting leaf tobaccos. The present invention encompasses (i) a tobacco plant into which a mutation for suppressing the development of primary axillary buds is introduced, (ii) a method of obtaining the tobacco plant, (iii) a harvest from the tobacco plant, and (iv) a processed product of the harvest.

The object of an embodiment of the present invention is to improve the quality of a tobacco product. The present invention relates to a tobacco plant in which a mutation is introduced in a genome, the mutation causing suppression of a function of a specific endogenous gene.

The object of an embodiment of the present invention is to improve the quality of a tobacco product. The present invention relates to a tobacco plant in which a mutation is introduced in a genome, the mutation causing suppression of a function of a specific endogenous gene.

Consumer products containing cultivar mint oil selected from the group consisting of ginger mint, blue balsam tea mint, macho mint, julep mint, banana mint, sweet pear mint, lavender mint, spearmint, peppermint and combinations thereof for a new sensory-organoleptic experience. Consumer products include chewing gum, confection, lozenge, edible film, pastilles, dentifrice, toothpaste, mouthwash, mouth spray, tobacco, pharmaceuticals, and edible films.

The present invention provides methodologies, including the utilization of genetically modified (GM) tobaccos, for the development and production of consumer acceptable PREPs encompassing the following: 1) production of low tar-to-nicotine ratio cigarettes, which allow smokers to obtain satisfying amounts of nicotine more efficiently than conventional cigarettes while reducing whole smoke deliveries; thereby reducing smoker “compensation” that exists with conventional low-yield cigarettes; 2) reduction of harmful tobacco-specific nitrosamines in tobacco products by genetic means and by extracting nicotine from tobacco and combining such with genetically modified reduced-nicotine tobacco; 3) production of improved expanded tobacco which utilizes genetically modified increased-nicotine tobacco; and 4) production of reconstituted tobacco which includes any combinations of the following: genetically modified increased-nicotine tobacco, genetically modified reduced-nicotine tobacco, tobacco leaf fractions, and freshly harvested freeze-dried tobacco.

The present invention provides methodologies, including the utilization of genetically modified (GM) tobaccos, for the development and production of consumer acceptable PREPs encompassing the following: 1) production of low tar-to-nicotine ratio cigarettes, which allow smokers to obtain satisfying amounts of nicotine more efficiently than conventional cigarettes while reducing whole smoke deliveries; thereby reducing smoker “compensation” that exists with conventional low-yield cigarettes; 2) reduction of harmful tobacco-specific nitrosamines in tobacco products by genetic means and by extracting nicotine from tobacco and combining such with genetically modified reduced-nicotine tobacco; 3) production of improved expanded tobacco which utilizes genetically modified increased-nicotine tobacco; and 4) production of reconstituted tobacco which includes any combinations of the following: genetically modified increased-nicotine tobacco, genetically modified reduced-nicotine tobacco, tobacco leaf fractions, and freshly harvested freeze-dried tobacco.

A cartridge may include a housing, a liquid supply reservoir in the housing and configured to store a pre-vapor formulation, a vaporizer, and a porous plug. The vaporizer may be in liquid communication with the liquid supply reservoir. The vaporizer may be configured to generate a vapor from heating a portion of the pre-vapor formulation. The porous plug may be connected to the housing and separated from the liquid supply reservoir. The porous plug may be permeable to the vapor. The porous plug may enclose a flavoring additive contacting a storage material. The flavoring additive may be configured to at least partially elute from the storage material or at least partially volatilize from the storage material if the vapor flows through the porous plug. An e-vaping device may include the cartridge.

A plant cell comprising: (a) a polynucleotide sequence encoding a nitrate reductase polypeptide comprising a contiguous polypeptide sequence of SEQ ID NO: 5 or SEQ ID NO: 7, wherein methionine is substituted for an amino acid that reduces nitrate reductase activity in the plant cell as compared to a control plant cell; (b) a polypeptide sequence encoded by the polynucleotide sequence set forth in (a); or (c) a construct, vector or expression vector comprising the polynucleotide sequence set forth in (b).