A method for preparing a tar extract from a discarded cigarette butt and use of the tar extract in a cigarette includes the following steps: (1) adding the discarded cigarette butt to an extraction solvent, and carrying out microwave-assisted extraction to obtain an extraction solution; and (2) centrifuging the extraction solution, carrying out silica gel column chromatography on the supernatant, carrying out elution by using a petroleum ether-ethyl acetate mixed solution as a mobile phase, and collecting the resulting elution fraction to obtain the tar extract. In the method, the tar is extracted from the discarded cigarette butt as a raw material. Through the microwave-assisted extraction and separation by the silica gel column chromatography, harmful substances in the tar of the cigarette butt are removed, and the components with aroma characteristics are retained, and the tar extract is applied to a low-end cigarette.

In general, this invention belongs to the tobacco production and processing sector and more specifically it refers to the method of producing cut rag tobacco for cigarettes and other products from green tobacco leaves by the innovative integration of the GLT and Primary processes, with lower investment and operational production costs, while generating cut rag tobacco with high filling power while maintaining a flavor approximately equal to that of the original tobacco.

A tobacco preparation method, where a tobacco material is prepared through at least one extrusion process including compressing the material with an increase in pressure and temperature and mechanically processing and abruptly flash drying the material at an extruder outlet, where the tobacco material includes a tobacco lamina material, where a tobacco preparation device includes a dosing conveyor and an extruder which compresses a tobacco material with an increase in pressure and temperature and mechanically process and abruptly flash dries the material at the extruder outlet, where the components are designated as a unit which can be modularly delimited for processing a tobacco material including a tobacco lamina material, where an arrangement of several tobacco preparation devices and a smoking article or cigarette manufacturing device includes such a device or such a device arrangement which is connected upstream of a cigarette maker or a secondary unit as a tobacco preparation module.

A tobacco preparation method, where a tobacco material is prepared through at least one extrusion process including compressing the material with an increase in pressure and temperature and mechanically processing and abruptly flash drying the material at an extruder outlet, where the tobacco material includes a tobacco lamina material, where a tobacco preparation device includes a dosing conveyor and an extruder which compresses a tobacco material with an increase in pressure and temperature and mechanically process and abruptly flash dries the material at the extruder outlet, where the components are designated as a unit which can be modularly delimited for processing a tobacco material including a tobacco lamina material, where an arrangement of several tobacco preparation devices and a smoking article or cigarette manufacturing device includes such a device or such a device arrangement which is connected upstream of a cigarette maker or a secondary unit as a tobacco preparation module.

A sheet-type aerosol generating article includes at least two aerosol generating sheets each having aerosol generating material and an inductively heatable susceptor The inductively heatable susceptor is positioned between the aerosol generating sheets and each of the aerosol generating sheets has an exposed surface. Methods for manufacturing sheet-type aerosol generating articles are also disclosed.

Methods of forming a tobacco pouch having two or more compartments are disclosed. The methods include forming a multi-compartment tobacco pouch with a vertical form fill seal machine.

A sheet-type aerosol generating article includes at least two aerosol generating sheets each having aerosol generating material and an inductively heatable susceptor The inductively heatable susceptor is positioned between the aerosol generating sheets and each of the aerosol generating sheets has an exposed surface. Methods for manufacturing sheet-type aerosol generating articles are also disclosed.

In one aspect, there is provided a mutant, non-naturally occurring or transgenic plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a member of the CLC family of chloride channels and having at least 60% sequence identity to SEQ ID NO:1 or SEQ ID NO:2 or SEQ ID NO:3 or SEQ ID NO:4 or SEQ ID NO:10 or SEQ ID NO:11; (ii) a polypeptide encoded by the polynucleotide set forth in (i); (iii) a polypeptide comprising, consisting or consisting essentially of a sequence encoding a member of the CLC family of chloride channels and having at least 60% sequence identity to SEQ ID NO:5 or SEQ ID NO:6 or SEQ ID NO:7 or SEQ ID NO:12 or SEQ ID NO:13 or SEQ ID NO:14; or (iv) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i); and wherein the expression or activity of the polynucleotide or the polypeptide is modulated as compared to a control plant and wherein the nitrate levels in the mutant, non-naturally occurring or transgenic plant containing the mutant, non-naturally occurring or transgenic plant cell are modulated as compared to the control plant containing the control plant cell.

In one aspect, there is provided a mutant, non-naturally occurring or transgenic plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a member of the CLC family of chloride channels and having at least 60% sequence identity to SEQ ID NO:1 or SEQ ID NO:2 or SEQ ID NO:3 or SEQ ID NO:4 or SEQ ID NO:10 or SEQ ID NO:11; (ii) a polypeptide encoded by the polynucleotide set forth in (i); (iii) a polypeptide comprising, consisting or consisting essentially of a sequence encoding a member of the CLC family of chloride channels and having at least 60% sequence identity to SEQ ID NO:5 or SEQ ID NO:6 or SEQ ID NO:7 or SEQ ID NO:12 or SEQ ID NO:13 or SEQ ID NO:14; or (iv) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i); and wherein the expression or activity of the polynucleotide or the polypeptide is modulated as compared to a control plant and wherein the nitrate levels in the mutant, non-naturally occurring or transgenic plant containing the mutant, non-naturally occurring or transgenic plant cell are modulated as compared to the control plant containing the control plant cell.

A tobacco preparation method, where a tobacco material is prepared through at least one extrusion process including compressing the material with an increase in pressure and temperature and mechanically processing and abruptly flash drying the material at an extruder outlet, where the tobacco material includes a tobacco lamina material, where a tobacco preparation device includes a dosing conveyor and an extruder which compresses a tobacco material with an increase in pressure and temperature and mechanically process and abruptly flash dries the material at the extruder outlet, where the components are designated as a unit which can be modularly delimited for processing a tobacco material including a tobacco lamina material, where an arrangement of several tobacco preparation devices and a smoking article or cigarette manufacturing device inlcudes such a device or such a device arrangement which is connected upstream of a cigarette maker or a secondary unit as a tobacco preparation module.