A method for efficiently manufacturing and fabricating microfluidic chips, where a base mold is formed to have positive-relief features used to cast an intermediary template chip with negative-relief features having dimensions of a scale in the micron range. The intermediary template chip is used to case a production mold, which is formed of a reinforced epoxy resin that, once hardened into a solid epoxy member, can withstand the structural pressures of a CNC machining system. The production mold can be refined by a CNC machining, where the refined production mold is then used to cast production chips to be used as microfluidic chips.

The present invention discloses compositions and methods for repair and reconstruction of defects and injuries to soft tissues. Some aspects of the disclosure provide methods for corneal reconstruction by applying an engineered bioadhesive, glycidyl methacrylate-substituted gelatin and a visible light activated photoinitiator in presence of visible light to the corneal defect.

Processes for fabricating structured, relatively large area, ultra-thin polymer films are disclosed. For instance, such a process may include spinning a thermoplastic polymer film onto an etched wafer that serves as a mold for the thermoplastic polymer film, baking the thermoplastic polymer film on a hotplate at a curing temperature, delaminating the thermoplastic polymer film in water, and peeling the thermoplastic polymer film from the etched wafer, producing a structured thermoplastic polymer film that has structures corresponding to areas where the wafer has been etched.

What is provided is a molding device which enables a molded article to be excellently released, has a region that is on a molding surface and exhibits excellent abrasion resistance by being treated to show mold release properties, and can suppress the deterioration of mold release properties. Also provided is a method for manufacturing a fiber-reinforced composite material molded article. A fiber-reinforced composite material molding device (101) includes a molding die (130) for obtaining a fiber-reinforced composite material molded article by molding a fiber-reinforced composite material prepared by impregnating a reinforcing fiber base material with a resin composition, in which a surface free energy of a portion or the entirety of cavity surfaces (113a) and (123a) of the molding die (130) is equal to or lower than 25.0 mJ/m.sup.2 which is measured by a three liquid method. A portion or the entirety of the cavity surfaces (113a) and (123a) is preferably an implanted surface to which either or both of fluorine and silicon are implanted.

A method for efficiently manufacturing and fabricating microfluidic chips, where a base mold is formed to have positive-relief features used to cast an intermediary template chip with negative-relief features having dimensions of a scale in the micron range. The intermediary template chip is used to case a production mold, which is formed of a reinforced epoxy resin that, once hardened into a solid epoxy member, can withstand the structural pressures of a CNC machining system. The production mold can be refined by a CNC machining, where the refined production mold is then used to cast production chips to be used as microfluidic chips.

There is provided a sealing apparatus for a cryopreservation bag, with which a sealing treatment of an inlet/outlet of the cryopreservation bag is carried out automatically and anyone can safely and properly carry out the sealing treatment. The sealing apparatus includes: a bag clamping device 56; a laser device 57; and a scanning structure 58 for moving the bag clamping device 56, for example. The bag clamping device 56 includes a fixed pinching block 67, a movable pinching block 69, and a clamp actuator 70. The laser device 57 includes a laser oscillator 104 and a condensing lens 107. The fixed pinching block 67 includes a block base 73, a heat radiator 74, and a heat radiator holder 75. An infrared laser beam is radiated to a sealed portion 55 of the bag to form a seal bead 125 for sealing in a state in which the sealed portion 55 is pinched and fixed by the heat radiator 74 and the movable pinching block 69 and while the bag clamping device 56, for example, is moved by the scanning structure 58.

There is provided a sealing apparatus for a cryopreservation bag, with which a sealing treatment of an inlet/outlet of the cryopreservation bag is carried out automatically and anyone can safely and properly carry out the sealing treatment. The sealing apparatus includes: a bag clamping device 56; a laser device 57; and a scanning structure 58 for moving the bag clamping device 56, for example. The bag clamping device 56 includes a fixed pinching block 67, a movable pinching block 69, and a clamp actuator 70. The laser device 57 includes a laser oscillator 104 and a condensing lens 107. The fixed pinching block 67 includes a block base 73, a heat radiator 74, and a heat radiator holder 75. An infrared laser beam is radiated to a sealed portion 55 of the bag to form a seal bead 125 for sealing in a state in which the sealed portion 55 is pinched and fixed by the heat radiator 74 and the movable pinching block 69 and while the bag clamping device 56, for example, is moved by the scanning structure 58.

Methods of fabricating ultra-miniature, ultra-compliant probe arrays through spin coating, wherein a dissolvable material in hydrogel form is dispensed onto an assembled mold with wires. Once the dissolvable material is dispensed onto the mold, centrifuging spin casts the material by evaporating the solvent, forming a dried dissolvable polymer. Finally, a device is used with water to remove excess dissolvable material to obtain a dissolvable needle with wires.

A method for manufacturing a microfluidic device includes following steps. A mold made of a glass material is provided. The mold has at least one hollow mold cavity and at least one blocking wall around the hollow mold cavity. The mold is disposed on a silicon substrate, which includes a formation surface corresponding to the hollow mold cavity and a microfluidic male mold protruding from the formation surface. Polydimethylsiloxane (PDMS) is poured into the hollow mold cavity and baked to harden the PDMS to form the microfluidic device. The microfluidic device has a microfluidic structure corresponding to the microfluidic male mold, and a height of a sidewall of the microfluidic device is between 3 mm and 30 mm. With the glass material of the mold, the microfluidic device having a sidewall height greater than 3 mm can be manufactured, preventing an insufficient suction force of a negative pressure.

The present invention discloses compositions and methods for repair and reconstruction of defects and injuries to soft tissues. Some aspects of the disclosure provide methods for corneal reconstruction by applying an engineered bioadhesive, glycidyl methacrylate-substituted gelatin and a visible light activated photoinitiator in presence of visible light to the corneal defect.