In one aspect, the present disclosure relates to a masked fluorogenic compound comprising a small molecule protecting group that can be cleaved following a reaction with a biomarker. In some embodiments, cleavage of the small molecule protecting group provides a fluorogenic ligand that binds to an aptamer, leading to fluorescence emission. In another aspect, the present disclosure relates to a method of detecting a disease or a disorder in a subject and/or in a biological sample from the subject.

The present invention provides a carboxylate-bridged binuclear iron-sulfur clusters fluorescent probe having the structure of Formula I. The preparation method comprises the following steps: anthracenylmethanamine and p-methoxycarbonylphenyl isocyanate have an addition reaction to get a substitutional methyl benzoate, which is hydrolyzed to get a corresponding carboxylic acid; react the resulting carboxylic acid with alkali to get carboxylate, and then coordinate with binuclear iron precursor to obtain the fluorescent probe. Compared with the prior art, the invention firstly provides the carboxylate-bridged binuclear iron-sulfur clusters of metal complex which similar to the central structure of bio-enzyme. The metal complex, as a fluorescent probe, has good selectivity to the fluorinion detection. The fluorescence titration experiment is simple and easy to operate and the fluorescence changes are sensitive. ##STR00001##

The present invention provides a carboxylate-bridged binuclear iron-sulfur clusters fluorescent probe having the structure of Formula I. The preparation method comprises the following steps: anthracenylmethanamine and p-methoxycarbonylphenyl isocyanate have an addition reaction to get a substitutional methyl benzoate, which is hydrolyzed to get a corresponding carboxylic acid; react the resulting carboxylic acid with alkali to get carboxylate, and then coordinate with binuclear iron precursor to obtain the fluorescent probe. Compared with the prior art, the invention firstly provides the carboxylate-bridged binuclear iron-sulfur clusters of metal complex which similar to the central structure of bio-enzyme. The metal complex, as a fluorescent probe, has good selectivity to the fluorinion detection. The fluorescence titration experiment is simple and easy to operate and the fluorescence changes are sensitive. ##STR00001##

The thiol group-containing colored compound includes a chromophore having absorption in a visible light region, a thiol group, and a spacer provided between the chromophore and the thiol group. The spacer is a chain alkylene group having 2 to 30 carbon atoms, a cyclic alkylene group having 3 to 30 carbon atoms, or a derivative of either alkylene group in which the number of carbon atoms in an additional structure of the alkylene group is equal to or less than the number of carbon atoms in the alkylene group.

The thiol group-containing colored compound includes a chromophore having absorption in a visible light region, a thiol group, and a spacer provided between the chromophore and the thiol group. The spacer is a chain alkylene group having 2 to 30 carbon atoms, a cyclic alkylene group having 3 to 30 carbon atoms, or a derivative of either alkylene group in which the number of carbon atoms in an additional structure of the alkylene group is equal to or less than the number of carbon atoms in the alkylene group.

A color filter comprises a red color filter, a green color filter, and a blue color filter, wherein the green color filter comprises a cyan pigment satisfying the following equation: $\frac{A2}{A1}=0$
where A1 denotes the absorbance of the cyan pigment in a wavelength range of about 600 nm to about 700 nm and A2 denotes the absorbance of the cyan pigment in a wavelength range of about 450 nm to about 500 nm.

A color filter comprises a red color filter, a green color filter, and a blue color filter, wherein the green color filter comprises a cyan pigment satisfying the following equation: $\frac{A2}{A1}=0$
where A1 denotes the absorbance of the cyan pigment in a wavelength range of about 600 nm to about 700 nm and A2 denotes the absorbance of the cyan pigment in a wavelength range of about 450 nm to about 500 nm.

The present invention provides a carboxylate-bridged binuclear iron-sulfur clusters fluorescent probe having the structure of Formula I. The preparation method comprises the following steps: anthracenylmethanamine and p-methoxycarbonylphenyl isocyanate have an addition reaction to get a substitutional methyl benzoate, which is hydrolyzed to get a corresponding carboxylic acid; react the resulting carboxylic acid with alkali to get carboxylate, and then coordinate with binuclear iron precursor to obtain the fluorescent probe. Compared with the prior art, the invention firstly provides the carboxylate-bridged binuclear iron-sulfur clusters of metal complex which similar to the central structure of bio-enzyme. The metal complex, as a fluorescent probe, has good selectivity to the fluorinion detection. The fluorescence titration experiment is simple and easy to operate and the fluorescence changes are sensitive.

##STR00001##

The present invention provides a carboxylate-bridged binuclear iron-sulfur clusters fluorescent probe having the structure of Formula I. The preparation method comprises the following steps: anthracenylmethanamine and p-methoxycarbonylphenyl isocyanate have an addition reaction to get a substitutional methyl benzoate, which is hydrolyzed to get a corresponding carboxylic acid; react the resulting carboxylic acid with alkali to get carboxylate, and then coordinate with binuclear iron precursor to obtain the fluorescent probe. Compared with the prior art, the invention firstly provides the carboxylate-bridged binuclear iron-sulfur clusters of metal complex which similar to the central structure of bio-enzyme. The metal complex, as a fluorescent probe, has good selectivity to the fluorinion detection. The fluorescence titration experiment is simple and easy to operate and the fluorescence changes are sensitive.

##STR00001##

A color filter comprises a red color filter, a green color filter, and a blue color filter, wherein the green color filter comprises a cyan pigment satisfying the following equation:

[00001]$\frac{A.Math..Math.2}{A.Math..Math.1}=0$

where A1 denotes the absorbance of the cyan pigment in a wavelength range of about 600 nm to about 700 nm and A2 denotes the absorbance of the cyan pigment in a wavelength range of about 450 nm to about 500 nm.