Apparatus for distributing a solid, gel, powder, granular, liquid or viscous liquid test substance in an extraction fluid comprises: a) a flow cell for holding the test substance comprising an extraction chamber of uniform cross-sectional area, a grinding surface, an extraction fluid inlet and an extract outlet; b) a grinding head disposed within the extraction chamber and adapted to reciprocate therein; and c) a recirculating pump for driving extraction fluid: (i) into the flow cell via the extraction fluid inlet; (ii) through the extraction chamber; and (iii) back to the flow cell via the extract outlet.

Disclosed are collagen nanoparticles obtained from waste scaly skins from fish by using a micro fluid technique, a method for obtaining the collagen nanoparticles, and their field of use.

Provided is an enzymatic method of extracting high quality gelatin from collagen containing material.

Provided is an enzymatic method of extracting high quality gelatin from collagen containing material.

The present invention relates to a method for producing bone gelatine having an isoelectric point of less than 6, comprising the following steps: a) providing bones of vertebrates; b) mechanically crushing the bones to a particle size of less than 1 500 μm, preferably less than 500 μm, more preferably less than 300 μm; c) extracting the crushed bones using an aqueous medium at a temperature of from 100 to 140° C., preferably from 120 to 130° C., for a period of from 0.5 to 10 min, preferably 1 to 5 min, more preferably 1 to 3 min; d) separating off the aqueous gelatine solution from the crushed bones; and e) drying the aqueous gelatine solution in order to obtain the bone gelatine having an isoelectric point of less than 6, wherein the method does not comprise liming of the bones with a base, and wherein the bones provided in step a) have not undergone liming. The invention further relates to bone gelatine having an isoelectric point of less than 6, produced by this method.

The present invention relates to a method for producing bone gelatine having an isoelectric point of less than 6, comprising the following steps: a) providing bones of vertebrates; b) mechanically crushing the bones to a particle size of less than 1 500 μm, preferably less than 500 μm, more preferably less than 300 μm; c) extracting the crushed bones using an aqueous medium at a temperature of from 100 to 140° C., preferably from 120 to 130° C., for a period of from 0.5 to 10 min, preferably 1 to 5 min, more preferably 1 to 3 min; d) separating off the aqueous gelatine solution from the crushed bones; and e) drying the aqueous gelatine solution in order to obtain the bone gelatine having an isoelectric point of less than 6, wherein the method does not comprise liming of the bones with a base, and wherein the bones provided in step a) have not undergone liming. The invention further relates to bone gelatine having an isoelectric point of less than 6, produced by this method.

A tissue repair material includes gelatin granules, and the tissue repair material exhibits a water absorptivity of 800% by mass or more, and a residual ratio of 60% by mass or less after three hours of decomposition treatment using 1 mol/L hydrochloric acid. A block-shaped tissue repair material includes gelatin, and the block-shaped tissue repair material exhibits a water absorptivity of 800% by mass or more, and a residual ratio of 60% by mass or less after three hours of decomposition treatment using 1 mol/L hydrochloric acid.

A gelatin or a chemically modified product thereof contains 10 to 50% by mass of a high-molecular weight component, and a low-molecular weight component in such an amount that a value obtained by subtracting a low-molecular weight component content from a high-molecular weight component content is greater than or equal to 0% by mass.

A gelatin or a chemically modified product thereof contains 10 to 50% by mass of a high-molecular weight component, and a low-molecular weight component in such an amount that a value obtained by subtracting a low-molecular weight component content from a high-molecular weight component content is greater than or equal to 0% by mass.

A method of preparing an aesculin sturgeon skin gelatin with antioxidant activity and Enterococcus faecalis detection ability includes: 1) mixing a sturgeon skin gelatin and distilled water in a ratio of 1:15-1:25 (w/v) at 50-70 C. and filtering to obtain a sturgeon skin gelatin solution; 2) adding aesculin and a glycerin solution to the sturgeon skin gelatin solution, stirring the resulted sturgeon skin gelatin solution at 30-50 C. for 30 minutes, and filtering; and 3) removing air bubbles from the sturgeon skin gelatin solution of step 2) under reduced pressure, placing the sturgeon skin gelatin solution on an acrylic glass, and drying the sturgeon skin gelatin solution at in a vented oven 25 C. and 45-55% relative humidity for 24 hours to obtain the aesculin sturgeon skin gelatin film.