The invention discloses a first connection unit having a plurality of sensor surfaces and which is adapted to be asepetically connected to a second connection unit mounted e.g. on a flexible bioreactor bag.

Some embodiments of the present disclosure comprise a flow diverter for use in a biocontainer or bioreactor, comprising a shoulder having a first surface and a second surface opposite the first surface, a conduit extending from the first surface of the shoulder, an inlet at a first end of the conduit, wherein the first end comprises a connector, an outlet formed within the second surface of the shoulder in fluid communication with the inlet at an end opposite the first end of the conduit and a hood adjacent to the outlet, wherein a fluid diverter is capable of directing a fluid down a sidewall of the biocontainer or bioreactor, attenuating a splashing or foaming condition.

In various embodiments a culture platform for cultivating tissue is provided, comprising: a first component (2) comprising at least one culture chamber (21) formed therein, the culture chamber (21) comprising a bottom (23), a sidewall and an open top (22); and a second component (2) comprising a base (31) and at least one pair of posts (32) extending from the base (31); wherein the first component (2) and the second component (3) are sized and configured to be mated with one another such that when the second component (3) is placed on the first component (2), the at least one pair of posts (32) is inserted into the at least one culture chamber (21). Furthermore, a method for observing tissue cultivated therein is provided.

A multi-sensor component for the installation of at least two sensors at an individual port of a container for culturing biological material is provided. The multi-sensor component has a housing that can be introduced by a front housing segment into an uptake opening extending through the port of the container so that the front housing segment is facing the inside of the container. The multi-sensor component has a first sensor unit or a mount for a first sensor unit arranged on the front housing segment and has a second sensor unit or a mount for a second sensor unit arranged on the front housing segment.

A rupture disk includes a body including an internal wall extending from a first end of the body to a second end of the body. The internal wall defines an opening extending through the body. In some embodiments, the body further includes an internal flange formed on the internal wall, and the internal flange has a stepped surface. A rupture membrane is positioned within the body, and secured, for example, by cold welding. The rupture membrane may be secured to the stepped surface. The rupture membrane is positioned within the body such that the rupture membrane does not extend beyond the first end of the body. A process system including the rupture disk is also provided. A method of maintaining a process system that includes the rupture disk is also provided.

Disclosed herein is an instrument suitable for processing cells for example culturing, concentrating or washing said cells, the instrument comprising: a housing for accommodating mechanical elements including at least one fluid pump; and a disposable processing kit complementary to the mechanical elements within the housing and comprising a fluid circuit including a fluid reservoir and plural fluid paths capable of carrying fluid flow caused by said pump(s), the instrument further including a mechanism for determining the quantity, or change in quantity of the fluid in the reservoir resulting from said fluid flow, the instrument yet further comprising a controller operable to control at least the pump and operable to perform a fault determination process, which includes the steps of determining the expected flow rate of said pump(s) calculated from the speed of the pump(s) and comparing that expected flow with the change in quantity of the fluid in the reservoir as determined by said mechanism.

A reactor system includes a support housing having an interior surface bounding a chamber, the chamber having a vertically extending central longitudinal axis. A flexible bag is disposed within the chamber of the support housing and has an interior surface bounding a compartment. A mixing element is disposed within the compartment of the flexible bag. A drive element, such as a drive shaft, is secured the mixing element, wherein the mixing element is laterally offset from and/or is angled relative to the vertically extending central longitudinal axis of the support housing.

The present invention provides a system for the insertion of a pre-sterilized sensor probe into a sterile vessel. The system of the invention provides a reliable and straightforward way to insert one or more sterile probes into a sterile vessel. The present invention also provides a sterile vessel that includes one or more of the systems of the invention. The sterile vessel can be a flexible or semi-rigid bag or tubing of the type typically used for carrying out biochemical and/or biological processes and/or manipulating liquids and other products of such processes. Furthermore, the present invention provides a method for aseptically inserting a probe into a sterile vessel where the method makes use of the system of the invention.

Disclosed is a well insert for cell culture, including: a membrane support having an upper end and a lower end, the upper end being adapted to engage a well of a microplate so as to suspend the well insert therein; and a permeable membrane for supporting a tissue culture, the permeable membrane being attached at the lower end of the membrane support and sealed thereto, the permeable membrane being of brittle material. The membrane support is overmolded or fastened on to the permeable membrane so as be sealed thereto.

A bioreactor sensing system is presented. The bioreactor sensing system comprises a bioreaction vessel. The bioreaction vessel comprises an aperture configured to provide an interface for monitoring contents within the bioreaction vessel. The bioreaction vessel also comprises a port coupled to the bioreaction vessel proximate the aperture. The bioreactor sensing system also comprises a sensing device disposed at least partially within the port such that a sensing element is exposed to the contents. The bioreactor sensing system also comprises an external seal configured to be applied over a portion of the sensing device and a portion of the port.