The present invention is related to a method to be performed with one tissue type, wherein a specific combination of hydrogel features has been pre-selected for the said one tissue type to be tested. The present invention is also related to a kit of parts to perform said method.

Methods to form a surface coating and surface pattern, which are based on adsorption of hydrocarbon chains that can be used with imaging optics to visualize macrophage fusion and multinucleated giant cell formation with living specimens are described.

The present disclosure provides a method of bioprinting a 3-D structure comprising one or more biologically-relevant materials on a super-hydrophobic surface. In one embodiment, the method comprises providing a composition having one or more biologically-relevant materials dispersed within a biocompatible medium. A pattern comprising a hydrophilic material is deposited on a defined area of the super-hydrophobic surface, wherein the pattern is modeled after a biological structure. The composition having the one or more biologically-relevant materials is then bioprinted atop the hydrophilic surface to form a 3-D structure, wherein the hydrophilic surface maintains the 3-D structure in a desired position or shape on the super-hydrophobic surface.

A method for producing a cell-containing extracellular matrix is provided, the method including preparing an extracellular matrix solution which contains (i) an extracellular matrix precursor and (ii) cells or a cell mass inside a pipette having a tip opening portion; discharging the extracellular matrix solution to form a drop of the extracellular matrix solution at the tip opening portion of the pipette; bringing the tip opening portion of the pipette close to a culture surface of a cell culture container to place the drop on the culture surface while avoiding bringing the tip opening portion of the pipette into contact with the culture surface; moving the tip opening portion of the pipette away from the culture surface to separate the drop from the tip opening portion of the pipette; and gelating the extracellular matrix solution to form a cell-containing extracellular matrix.

The invention provides a method for preparing an ordered cell-containing microarray, and a system for preparing an ordered cell-containing microarray.

The present invention relates to a method for producing biodegradable fibers on the basis of a silane compound, said silane compound being crosslinked during production and, at least to some extent, an organic acid being incorporated into the forming crosslinked structure via covalent bonds and/or contributing to the crosslinking. The present invention also relates to the fibers that can be produced by the method according to the invention and to the use thereof.

The method of culturing cells disclosed herein includes printing cells onto a substrate that includes cell adhesive regions and cell repulsive regions. The cells are suspended in a printing medium to create a cell suspension, and a volume of the cell suspension is loaded into a printer. A cell adhesive region of the substrate is aligned beneath the printing channel of the printer, and droplets of the cell suspension are dispensed from the printing channel directly onto the cell adhesive region. Contact of the dispensed droplets with cell repulsive regions of the substrate is limited, either by targeting of the droplets to the cell adhesive regions, by repulsions generated by the cell repulsive areas, or both. The cells adhere to the cell adhesive regions to create a cell pattern, and are maintained thereafter in a physiologically suitable environment.

The present invention discloses a partially crosslinked hydrogels blended composition with enhanced viscosity and yield stress, which is formed by the polymerization of one or more colloid monomers through crosslinking. The polymerization is initiated by a photoinitiator under irradiation of the light of a specific wavelength, which promotes crosslinking of the one or more colloid monomers. The hydrogels blended composition can be further crosslinked with one or more other colloid monomers through repeated excitation of the photoinitiator. The hydrogels blended composition can be polymerized into a gel upon re-irradiation, and can also be used as a biomaterial for wound repair, three-dimensional cell culture, personal nursing care, health care, medical and pharmaceutical applications.

Materials and methods for cell-mimetics having mechanical properties of biological neural axons are provided. A cell-mimetic device includes an array of fibers comprised of hexanediol diacrylate (HDDA) or an HDDA derivative, and at least one derivative of polyethylene glycol (PEG) selected from the group consisting of: PEG-acrylate, PEG-diacrylate, and any multi-arm PEG-acrylate.

The present disclosure provides a composite material. The composite material comprises nanoparticles and a flexible substrate, the nanoparticles comprise one or more of carbon nanotubes, graphene, gold nanoparticles, and polydopamine nanoparticles, the flexible substrate comprises one or more of thermosetting plastics such as polydimethylsiloxane and a hydrogel, and the mass percentage of the nanoparticles in the composite material is 0 to 60‰. The composite material of the present disclosure is easy to prepare, has extremely strong photothermal conversion performance, and does not change the smooth surface of an original topological structure. Meanwhile, the composite material has universality and versatility for different cells, the delivery efficiency is close to 100%, and modified cells may be efficiently and non-destructively released and harvested by means of traditional trysinization, and the harvesting efficiency is 90% or more.