Provided herein, inter alia, are methods and compositions for inhibiting DNase II-mediated degradation of reporter nucleic acid molecules coupled to antigens of interest, and thus improving sensitivity of single-cell immune profiling workflows,

Provided herein, inter alia, are methods and compositions for inhibiting DNase II-mediated degradation of reporter nucleic acid molecules coupled to antigens of interest, and thus improving sensitivity of single-cell immune profiling workflows,

Disclosed herein include methods, compositions, and kits for use in detecting a target nucleic acid sequence in a sample. Provided include acidic compositions for separating dsDNA. In some embodiments, the acidic compositions comprise a monovalent salt and/or a divalent salt, one or more surfactants, and an acidic agent. In some embodiments, the pH of the acidic composition is less than 4. Also provided herein are reagent compositions comprising a buffering agent and one or more amplification agents.

Disclosed herein include methods, compositions, and kits for use in detecting a target nucleic acid sequence in a sample. Provided include acidic compositions for separating dsDNA. In some embodiments, the acidic compositions comprise a monovalent salt and/or a divalent salt, one or more surfactants, and an acidic agent. In some embodiments, the pH of the acidic composition is less than 4. Also provided herein are reagent compositions comprising a buffering agent and one or more amplification agents.