Aspects of the present disclosure include methods and systems for automated analysis of clinical fluid samples, such as urine, blood, or cerebral spinal fluid, where the number of fluid samples in increased or optimized without negatively impacting the accuracy of the analysis of a given fluid sample.

Aspects of the present disclosure include methods and systems for automated analysis of clinical fluid samples, such as urine, blood, or cerebral spinal fluid, where the number of fluid samples in increased or optimized without negatively impacting the accuracy of the analysis of a given fluid sample.

A method for promoting biological activity uses a filter system to increase cell production of a fed batch bioreactor. The filter system cycles bioreactor fluid through a hollow fiber tangential flow filter which separates metabolic wastes (as well as proteins) from cells produced in bioreactor and returned to fed batch bioreactor, improving cell production in the fed batch bioreactor. The filter system includes a disposable pump and filter, and a reusable control system. The pump is a low shear gamma stable pump gently cycling bioreactor fluid through the filter with minimal damage to the cells produced in the bioreactor. The pumphead and hollow fiber tangential flow filter are disposable. The pump motor is part of the control system and is reusable. The pumphead and filter are provided as an assembled and pre-sterilized unit allowing simple and quick attachment to the fed batch bioreactor, and simple and quick disposal.

A method for promoting biological activity uses a filter system to increase cell production of a fed batch bioreactor. The filter system cycles bioreactor fluid through a hollow fiber tangential flow filter which separates metabolic wastes (as well as proteins) from cells produced in bioreactor and returned to fed batch bioreactor, improving cell production in the fed batch bioreactor. The filter system includes a disposable pump and filter, and a reusable control system. The pump is a low shear gamma stable pump gently cycling bioreactor fluid through the filter with minimal damage to the cells produced in the bioreactor. The pumphead and hollow fiber tangential flow filter are disposable. The pump motor is part of the control system and is reusable. The pumphead and filter are provided as an assembled and pre-sterilized unit allowing simple and quick attachment to the fed batch bioreactor, and simple and quick disposal.

A system and method for automated testing a sample of a body fluid for the presence of bacteria is described. The system includes a fluid handling device, Incubator, flow cytometer, at least a processor, and a memory configuring the at least a processor to distribute a portion of the plurality of fluid samples within a well plate to at least a first well, divide the portion of the plurality of fluid samples from the at least a first well into at least two wells including a T.sub.0 well and a T.sub.1 well, obtain a T.sub.0 enumerative baseline bacterial value at time T.sub.0, culture the fluid samples in the T.sub.1 well using the incubator, obtain a T.sub.1 enumerative control bacterial value at time T.sub.1, and determine a presence of bacteria as a function of the T.sub.0 enumerative baseline bacterial value and the T.sub.1 enumerative control bacterial value.

A system and method for automated testing a sample of a body fluid for the presence of bacteria is described. The system includes a fluid handling device, Incubator, flow cytometer, at least a processor, and a memory configuring the at least a processor to distribute a portion of the plurality of fluid samples within a well plate to at least a first well, divide the portion of the plurality of fluid samples from the at least a first well into at least two wells including a T.sub.0 well and a T.sub.1 well, obtain a T.sub.0 enumerative baseline bacterial value at time T.sub.0, culture the fluid samples in the T.sub.1 well using the incubator, obtain a T.sub.1 enumerative control bacterial value at time T.sub.1, and determine a presence of bacteria as a function of the T.sub.0 enumerative baseline bacterial value and the T.sub.1 enumerative control bacterial value.

The invention is directed to a control method for a process to convert bisulphide to elemental sulphur in an aqueous solution comprising sulphide-oxidising bacteria wherein the process is controlled by applying a potential between the anode electrode and the cathode electrode or between the anode electrode and the reference electrode of an electrochemical cell resulting in a current between the cathode electrode and the anode electrode, measuring a current as measured by an electrochemical cell and adapting the process in response to the measured current. The process to convert bisulphide may comprise the following steps: (a) contacting bisulphide with oxidised sulphide-oxidising bacteria in the aqueous solution and elemental sulphur, (b) oxidizing the reduced sulphide-oxidising bacteria, (c) using the oxidised sulphide-oxidising bacteria obtained in step (b) in step (a) and (d) isolating elemental sulphur from the aqueous solution obtained in step (a) and/or step (b).

The invention is directed to a control method for a process to convert bisulphide to elemental sulphur in an aqueous solution comprising sulphide-oxidising bacteria wherein the process is controlled by applying a potential between the anode electrode and the cathode electrode or between the anode electrode and the reference electrode of an electrochemical cell resulting in a current between the cathode electrode and the anode electrode, measuring a current as measured by an electrochemical cell and adapting the process in response to the measured current. The process to convert bisulphide may comprise the following steps: (a) contacting bisulphide with oxidised sulphide-oxidising bacteria in the aqueous solution and elemental sulphur, (b) oxidizing the reduced sulphide-oxidising bacteria, (c) using the oxidised sulphide-oxidising bacteria obtained in step (b) in step (a) and (d) isolating elemental sulphur from the aqueous solution obtained in step (a) and/or step (b).

Techniques for predicting an amount of at least one biomaterial produced or consumed by a biological system in a bioreactor are provided. Process conditions and metabolite concentrations are measured for the biological system as a function of time. Metabolic rates for the biological system, including specific consumption rates of metabolites and specific production rates of metabolites are determined. The process conditions and the metabolic rates are provided to a hybrid system model configured to predict production of the biomaterial. The hybrid system model includes a kinetic growth model configured to estimate cell growth as a function of time and a metabolic condition model based on metabolite specific consumption or secretion rates and select process conditions, wherein the metabolic condition model is configured to classify the biological system into a metabolic state. An amount of the biomaterial based on the hybrid system model is predicted.

Techniques for predicting an amount of at least one biomaterial produced or consumed by a biological system in a bioreactor are provided. Process conditions and metabolite concentrations are measured for the biological system as a function of time. Metabolic rates for the biological system, including specific consumption rates of metabolites and specific production rates of metabolites are determined. The process conditions and the metabolic rates are provided to a hybrid system model configured to predict production of the biomaterial. The hybrid system model includes a kinetic growth model configured to estimate cell growth as a function of time and a metabolic condition model based on metabolite specific consumption or secretion rates and select process conditions, wherein the metabolic condition model is configured to classify the biological system into a metabolic state. An amount of the biomaterial based on the hybrid system model is predicted.