The present invention pertains to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucleotides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.

The disclosure provides compositions, and methods of use thereof, for vaccines for treatment of gonococcal and/or meningococcal infection, comprising native outer membrane vesicle (NOMV) derived from bacteria containing a gonococcal protein that is a lipoprotein or is modified to be a lipoprotein. Also provided are meningococcal strains containing a gene encoding a gonococcal protein that is a lipoprotein or is modified to be a lipoprotein.

Described herein are novel methods for the acetyl transferase-catalyzed production of drimanyl-acetate compounds by the acetylation of the respective drimanyl alcohol sources performed in vitro or in vivo. Also described herein is the identification of enzymes having corresponding acetyl transferase activity from different microbial and plant sources. Also described herein is the provision of enzyme mutants derived from the newly identified enzymes. Further described herein is the provision of corresponding coding sequences of such enzymes and mutants, recombinant vectors, and recombinant host cells suitable for the production of such acetyl transferases and mutants and for performing the novel production methods of drimanyl acetate compounds. Still further described herein is a method of using such drimanyl acetates as intermediates for the production of odorant, flavor or fragrance or insect/pest control ingredients.

The present invention relates to biocatalysts catalyzing the formation of α-olefins. In particular, the invention provides polypeptides with improved decarboxylase activity on C.sub.10-C.sub.16 free fatty acids, more particularly on C.sub.10 or C.sub.12 free fatty acids, as compared to the P450 fatty acid decarboxylase isolated from the Staphylococcus massiliensis strain S46 (Sm46). The invention further provides recombinant nucleic acids and vectors comprising the coding sequences encoding these polypeptides, genetically engineered host cells expressing said polypeptides and methods for the production of C.sub.9-C.sub.15 α-olefins, more particularly C.sub.9 or C.sub.11 α-olefins, using said polypeptides or said host cells.

The present invention includes systems, methods and compositions for the in vivo bioconversion of water-soluble acetylated cannabinoid glycosides in plant cell suspension cultures.

The present invention includes systems, methods and compositions for the in vivo bioconversion of water-soluble acetylated cannabinoid glycosides in plant cell suspension cultures.

The present invention provides for novel compositions and methods of producing modified lignin by modulating expression of acyltransferases.

The present disclosure provides methods for identifying anthocyanins with improved stability, color, or hue using a screening method, and producing anthocyanins with improved stability, color, or hue in, for example, host cells comprising one or more heterologous glycosyltransferase nucleic acid molecules and one or more heterologous acyltransferase nucleic acid molecules.

The present invention includes systems, methods and compositions for the in vivo bioconversion of water-soluble cannabinoid glycosides in cell cultures.

The present disclosure relates to mutated genes of the LUX operon and their use in producing autoluminescent plants and bacteria exhibiting improved light output.