A method to recover an extracellular vesicle at a high efficiency, including (a) and (b): wherein (a) is mixing (i) an extracellular vesicle-containing sample, (ii) particles on which a substance having an affinity to extracellular vesicle membrane is immobilized, and (iii) a polymer to give a mixture solution containing (i′) target particles bound to the extracellular vesicle via the substance and (ii′) the polymer; and (b) separating the target particles from the mixture solution. The method further includes reducing a viscosity of the mixture solution between (a) and (b). A method for analyzing an extracellular vesicle. A kit having (a) a polymer, (b) a substance having an affinity to the extracellular vesicle membrane, and (c) an enzyme capable of degrading a polymer.

The present disclosure provides a three-dimensional hydrogel-graphene-based biosensor and a preparation method thereof, belonging to the technical field of biosensors. The present disclosure provides a three-dimensional hydrogel-graphene-based biosensor, including a substrate, an electrode layer, a graphene film, and a three-dimensional hydrogel material layer that are stacked in sequence; where the three-dimensional hydrogel material layer is formed of a hydrogel material having a three-dimensional network structure; the hydrogel material is obtained by polymerization of raw materials including an acrylamide monomer and a modified probe molecule; and the modified probe molecule is a probe molecule modified with an acrylamide group. The three-dimensional hydrogel-graphene-based biosensor has a desirable stability and a high sensitivity.

Subject of the invention is a composition comprising at least one fragment of the peptide ESAT-6 and at least one fragment of the peptide CFP-10. Preferably, the fragments comprise at least two sets of peptides, a first set comprising at least one peptide of from about 7 to 14 amino acid residues in length and a second set comprising at least one peptide of from 16 amino acid residues or greater. The invention also relates to diagnostic methods using the composition.

The present invention relates to methods for the detection of an analyte, such as coronavirus (e.g. SARS-CoV-2) or other virus particles and proteins, in a test sample. The invention also provides a flow device for use in such methods. Additionally, there is provided a coronavirus-binding reagent having the structure [sialic acid]-[linker]-[polymer]-[gold nanoparticle] for use in the devices and methods of the invention.

The invention relates to a conjugate comprising a binding element and a self-polymerising biopolymer. The invention also relates to a kit comprising the conjugate and self-polymerising biopolymer subunits. The invention also relates to a method of using the conjugate to measure the concentration of a target substance comprising binding the conjugate to the substance, isolating the bound conjugate, polymerising biopolymer filament, and calculating the concentration of the target substance.

Subject of the invention is a composition comprising at least one fragment of the peptide ESAT-6 and at least one fragment of the peptide CFP-10. Preferably, the fragments comprise at least two sets of peptides, a first set comprising at least one peptide of from about 7 to 14 amino acid residues in length and a second set comprising at least one peptide of from 16 amino acid residues or greater. The invention also relates to diagnostic methods using the composition.

The present disclosure provides a three-dimensional hydrogel-graphene-based biosensor and a preparation method thereof, belonging to the technical field of biosensors. The present disclosure provides a three-dimensional hydrogel-graphene-based biosensor, including a substrate, an electrode layer, a graphene film, and a three-dimensional hydrogel material layer that are stacked in sequence; where the three-dimensional hydrogel material layer is formed of a hydrogel material having a three-dimensional network structure; the hydrogel material is obtained by polymerization of raw materials including an acrylamide monomer and a modified probe molecule; and the modified probe molecule is a probe molecule modified with an acrylamide group. The three-dimensional hydrogel-graphene-based biosensor has a desirable stability and a high sensitivity.

Lyophilized chromophoric polymer dot compositions are provided. Also disclosed are methods of making and using the lyophilized compositions, methods of dispersing the lyophilized compositions in aqueous solutions and kits supplying the compositions.

A diagnostic method suitable for detection and quantification of blood-related diseases or conditions. The methods utilize biomarkers, such as hemoglobin and hemozoin as catalysts in an atom transfer radical polymerization (ATRP) reaction performed above a lower critical solution temperature (LCST) of a polymer which allows the polymerization to be tracked by rate of turbidity formation. The rate of turbidity formation is correlated to the concentration of the biomarker, making the tests useful quantitative techniques which can be utilized as point-of-care tests in the field.

The compositions described herein include a substrate, wherein the substrate is a metal, metal oxide, metal nitride or a silicon containing material; a self-assembled monolayer (SAM) bonded to the substrate, wherein the self-assembled monolayer comprises: a surface binding unit bonded to the substrate, wherein the surface binding unit is selected from the group consisting of hydroxamates, phosphonates, catechols, halosilanes, alkoxysilanes, phosphonic acids, alkenes, alkynes, alcohols, 1,2-diols, and thiols; a separator unit bonded to the surface binding unit; a mass altering unit bonded to the separator unit; and a detector unit bonded to the separator unit.