C12Y302/01008

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Method For Improving Yield Of Sprayed Corn Bran In Corn Wet-Milling Process
20220205006 · 2022-06-30 · ·

Disclosed is a method for improving the yield of sprayed corn bran in a corn wet-milling process, an enzyme preparation is added in the process of separating fiber from starch and protein, the fiber residue after the enzyme preparation treatment contains less water, less starch and/or protein residue, and has a looser and more fluffy structure, the yield of the sprayed corn bran in the corn wet-milling process can be remarkably improved while ensuring normal color of the finished sprayed corn bran, that is, the amount of concentrated corn soaking solution sprayed on the fiber corn bran is significantly increased.

STRAIN OF TRICHODERMA REESEI AND CULTURE METHOD AND USE THEREOF
20220186272 · 2022-06-16 ·

The invention relates to a strain of Trichoderma reesei BLCY-007 and its application in the production of xylooligosaccharides.

COMPOSITIONS COMPRISING POLYPEPTIDES HAVING XYLANASE ACTIVITY AND POLYPEPTIDES HAVING ARABINOFURANOSIDASE ACTIVITY

The present invention relates to compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity for use in, e.g., animal feed. The present invention further relates to polypeptides having arabinofuranosidase activity, polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Production of Par-Baked Products with Improved Freshness Employing Combination of GH8 Xylanase and Phospholipase
20220159975 · 2022-05-26 · ·

A method of producing a par-baked product dough, comprising incorporating into the dough a phospholipase enzyme and a GH8 xylanase.

NOVEL BIFIDOBACTERIUM GENUS BACTERIUM HAVING HIGH CAPACITY TO UTILIZE POLYSACCHARIDES OF DIETARY ORIGIN

Provided is a novel Bifidobacterium strain capable of utilizing xylans. Bacteria belonging to the genus Bifidobacterium having a xylanase gene on a genome.

FEED ADDITIVE FORMULATION AND METHODS OF MAKING AND USING THE SAME
20220133816 · 2022-05-05 ·

The presently disclosed subject matter relates to feed additive formulations for monogastric animal feed. Particularly, the disclosed formulations comprise an isolated xylanase enzyme and a B. licheniformis strain PWD-1. The feed additive formulations may further include B. amyloliquefaciens strain Ba-BPD1. The disclosed formulations are useful for addition to feeds for monogastric animals to synergistically improve the performance of the animals.

Polypeptide having xylanase activity

The present invention relates to an isolated polypeptide having xylanase activity. Also disclosed are isolated polynucleotides encoding the polypeptide, recombinant host cells expressing the polypeptide, and methods for degrading lignocellulosic biomass using the polypeptide. He invention finds utility in the production of biofuels, in the paper and pulp industry, in clothing or leather softening, in the food industry such as baking, etc.

GH5 and GH30 in Wet Milling

The instant application provides methods to improve the total starch yield and/or gluten yield from corn kernels in a wet milling process, the method comprising admixing corn kernels or a fraction of the corn kernels with an enzyme composition comprising an effective amount of one or more hydrolytic enzymes, wherein at least one of said hydrolytic enzymes is selected from the group consisting of a GH30 polypeptide, a GH5 polypeptide or a combination thereof.

PROCESS OF EXTRACTING OIL FROM THIN STILLAGE

A process of recovering oil, comprising (a) converting a starch-containing material into dextrins with an alpha-amylase; (b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar; (c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism; (d) recovering the fermentation product to form a whole stillage; (e) separating the whole stillage into thin stillage and wet cake; (e′) optionally concentrating the thin stillage into syrup; (f) recovering oil from the thin stillage and/or optionally the syrup, wherein a phospholipase is present and/or added during steps (a) to (c). Use of phospholipase for increasing oil recovery yields from thin stillage and/or syrup in a fermentation product production process.