The present invention provides compositions and formulations comprising glutathione with or without thiocyanate and methods of use thereof to treat diseases and disorders in mucosal/epithelial tissue.

The invention provides a method for combating biofilm, said method comprising contacting a biofilm with a composition comprising an effective amount of antimicrobial peptide biofilm enzyme combinations, preferably in the form of a fusion protein. The biofilm may be on an animate or inanimate surface and both medical and non-medical uses and methods are provided. In one aspect the invention provides a composition for use in the treatment or prevention of a biofilm in a subject, particularly in the oral cavity.

The present invention provides compositions and formulations comprising glutathione with or without thiocyanate and methods of use thereof to treat diseases and disorders in mucosal/epithelial tissue.

An enzymatically produced soluble -glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble -glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble -glucan fiber are also provided.

The present invention provides compositions and formulations comprising glutathione with or without thiocyanate and methods of use thereof to treat diseases and disorders in mucosal/epithelial tissue.

An enzymatically produced soluble -glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble -glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble -glucan fiber are also provided.

An enzymatically produced soluble -glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble -glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble -glucan fiber are also provided.

Systems and methods for eradicating biofilms by killing bacteria within a biofilm, degrading the matrix and removing biofilm debris are disclosed herein. The disclosed subject matter provides techniques for administering a suspension of H.sub.2O.sub.2 and iron oxide nanoparticles to substantially eradicate bacteria within a biofilm matrix and degrade the bio film matrix, actuating the iron oxide nanoparticles for assembly into biohybrid robots suitable for removal of biofilm debris, and moving the biohybrid robots to remove the bio film debris from accessible or enclosed surfaces. In some embodiments, the disclosed subject matter can include embedding iron oxide nanoparticles in a hydrogel to form a soft robotic structure, administering the soft robotic structure to a biofilm-covered location, and magnetizing the soft robot structure to substantially eradicate bacteria within a biofilm matrix, degrade the biofilm matrix, and remove biofilm debris from enclosed surfaces.

The present invention relates to polypeptides having endo-1,3--glucanase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, host cells comprising the polynucleotides, methods of producing the polypeptides, as well as oral care compositions comprising the polypeptides.