Provided herein is an -fucosidase that can cleave a conjugate comprising an N-glycan and a label where the label is added by amine reactive chemistry. The -fucosidase also has an accelerated reaction time using Schiff base labeled N-glycans compared with bovine kidney fucosidase. A reaction mix, enzyme mix and kit comprising the -fucosidase are provided, as well as a method for analyzing glycoproteins. The -fucosidase finds particular use in analyzing the N-glycans of therapeutic glycoproteins.

The present invention relates to use of a B. longum transitional microorganism that has a preferential utilization of 3-FL over 2FL. It also relates to methods and to a composition comprising the B. longum transitional microorganism and uses thereof.

Provided herein is an -fucosidase that can cleave a conjugate comprising an N-glycan and a label where the label is added by amine reactive chemistry. The -fucosidase also has an accelerated reaction time using Schiff base labeled N-glycans compared with bovine kidney fucosidase. A reaction mix, enzyme mix and kit comprising the -fucosidase are provided, as well as a method for analyzing glycoproteins. The -fucosidase finds particular use in analyzing the N-glycans of therapeutic glycoproteins.