The present disclosure provides food supplements comprising proteases that can digest a variety of food proteins to enhance their protein bioavailability in the gut.

The present disclosure provides food supplements comprising proteases that can digest a variety of food proteins to enhance their protein bioavailability in the gut.

The present disclosure provides food supplements comprising proteases that can digest a variety of food proteins to enhance their protein bioavailability in the gut.

The present disclosure provides food supplements comprising proteases that can digest a variety of food proteins to enhance their protein bioavailability in the gut.

The present disclosure provides proteolytic enzymes, i.e., S53 family proteases, having increased thermostability, increased enzymatic activity, and/or increased substrate specificity and which can digest a variety of food proteins, thereby enhancing a food's protein bioavailability. The S53 family proteases have amino acid sequences that are at least 80% identical to SEQ ID NO: 1 and comprises at least one mutation in the amino acid sequence's 513-loop which corresponds to amino acids 513 to 517 of SEQ ID NO: 1, wherein the at least one mutation is located at position 513, 514, 515, and/or 517.