A process for producing taurine. Where the taurine is produced from O-acetyl-L-serine (OAS) using biotransformation. In a first processing step (biotransformation 1), L-cysteic acid is produced from OAS using an enzyme selected from a class of OAS sulfhydrylases (EC 4.2.99.8) in the presence of a salt of sulfurous acid. Where the biotransformation is carried out under active pH control. In a second processing step (biotransformation 2), L-cysteic acid is decarboxylated to taurine. Where the OAS concentration in the batch is at least 10 g/L and the OAS sulfhydrylase is CysM.

The present disclosure provides a variant polypeptide having an activity of aspartate 1-dicarboxylase; a microorganism including same; a composition for producing beta-alanine or beta-alanine-derived compounds, the composition comprising the microorganism; and a method for producing beta-alanine or beta-alanine-derived compounds, the method comprising a step for culturing the microorganism.

The present disclosure provides compositions and methods for the biosynthetic production of carnosine and beta-alanine.

One or more genes in a biosynthesis pathway for a vitamin or other essential nutrient which is needed for the survival of a microorganism can be used as an effective selective marker to identify cells transformed with an exogenous nucleic acid. The microorganism does not naturally contain or express the one or more gene. This permits genetic manipulations to be performed. It permits lower cost fermentations to be performed. It permits production of the essential nutrient for subsequent commodity use.

The present application provides: a microorganism with enhanced activity of aspartate 1-decarboxylase derived from Tribolium castaneum; a composition for producing beta-alanine and/or a beta-alanine-derived compound, the composition comprising the microorganism; and a method for producing beta-alanine and/or a beta-alanine-derived compound, the method comprising a step for culturing the microorganism. The productivity of beta-alanine and/or a beta-alanine-derived compound is excellent.

Provided are N-terminally truncated variants of insect aspartate 1-decarboxylases that exhibit improved performance for beta-alanine production.

The present application relates to the field of microorganisms and specifically relates to highly active aspartate decarboxylases for the production of vitamin B5. In the present application, the L-aspartate -decarboxylase derived from Bacillus licheniformis was screened, the PanD with significantly higher activity which catalyze the production of -alanine than other orgins of PanD. The engineering bacterium for the fermentative production of vitamin B5 was constructed by applying PanD derived from B. licheniformis. The bottleneck of -alanine metabolism in the biosynthesis of vitamin B5 was lifted. Compared with the highly polluting chemical method for the production of vitamin B5, the biological method for the production of vitamin B5 of the present application has the advantages of renewable raw materials, easy treatment and resource utilization of waste residue, waste water and waste gas, and thus can be used in practice for the industrial production of vitamin B5, which is of significant application value.

Methods for the fermentative production of O-phosphoserine, cysteate, or taurine in microbes or unicellular organisms that contain a serB mutation that either decreases serB expression, reduces the amount of the serB gene product or results in a serB gene product with low enzymatic activity. Genetic modifications of the O-phosphoserine, cysteate, or taurine and/or substrate biosynthetic pathways in unicellular organisms that include bacteria, algae, microalgae, diatoms, yeast, or fungi are disclosed. Also disclosed are fermentation and processing methods for the production of various O-phosphoserine-, cysteate-, or taurine-containing products and the use of the cells, fermentation broth or extracts that contain O-phosphoserine, cysteate, or taurine to produce products for use in food, feed, beverages, dietary and health supplements, cosmetics, personal care, pharmaceuticals, agricultural production, or surfactants.